Host Zinc-finger CCHC-type containing protein 3 inhibits pseudorabies virus proliferation by regulating type I interferon signaling

Gene, Год журнала: 2022, Номер 827, С. 146480 - 146480

Опубликована: Апрель 5, 2022

Язык: Английский

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Expression of sex-specific molecular markers by Babesia bovis gametes

Parasites & Vectors, Год журнала: 2024, Номер 17(1)

Опубликована: Фев. 19, 2024

Abstract Background Bovine babesiosis caused by Babesia bovis is one of the most important tick-borne diseases cattle in tropical and subtropical regions. parasites have a complex lifecycle, including development within mammalian host tick vector. In midgut, extracellular transform into gametes that fuse to form zygotes. To date, little known about genes proteins expressed male gametes. Methods results We developed method separate from vitro induced B. culture. Separation enabled validation sex-specific markers. Collected gametocytes were observed Giemsa-stained smear live-cell fluorescence microscopy. used confirm markers quantitative real-time PCR. Some found be gamete specific pka , hap2 α-tubulin II znfp2 . However, I ABC transporter, trap2-4 ccp1-3 upregulated culture depleted (female-enriched). Live immunofluorescence analysis using polyclonal antibodies confirmed surface expression HAP2 TRAP2-4 female These revealed strong distinguish between Conclusions Herein, we describe identification molecular essential for sexual reproduction. tools will enhance our understanding biology stages and, consequently, additional strategies control bovine babesiosis. Graphical

Язык: Английский

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SARS-CoV-2 disrupts host gene networks: Unveiling key hub genes as potential therapeutic targets for COVID-19 management

Computers in Biology and Medicine, Год журнала: 2024, Номер 183, С. 109343 - 109343

Опубликована: Ноя. 4, 2024

Язык: Английский

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ZC3H11A loss of function enhances NF-κB signaling through defective IκBα protein expression

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Ноя. 9, 2022

ZC3H11A is a cellular protein associated with the transcription export (TREX) complex that induced during heat-shock. Several nuclear-replicating viruses exploit mRNA mechanism of for their efficient replication. Here we show plays role in regulation NF-κB signal transduction. Depletion resulted enhanced mediated signaling, upregulation numerous innate immune related mRNAs, including IL-6 and large group interferon-stimulated genes. absence correlated an increased factor binding to promoter decreased decay. The signaling pathway deficient cells defect IκBα inhibitory accumulation. Upon depletion was retained cell nucleus resulting failure maintain normal levels cytoplasmic essential its feedback loop on activity. These findings indicate towards previously unknown regulating at level IkBα export.

Язык: Английский

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Functional Characterization of Eight Zinc Finger Motif-Containing Proteins in Toxoplasma gondii Type I RH Strain Using the CRISPR-Cas9 System

Pathogens, Год журнала: 2023, Номер 12(10), С. 1232 - 1232

Опубликована: Окт. 11, 2023

The Zinc finger protein (ZFP) family is widely distributed in eukaryotes and interacts with DNA, RNA, various proteins to participate many molecular processes. In the present study, biological functions of eight ZFP genes lytic cycle pathogenicity Toxoplasma gondii were examined using CRISPR-Cas9 system. Immunofluorescence showed that four ZFPs (RH248270-HA, RH255310-HA, RH309200-HA, RH236640-HA) localized cytoplasm, one (RH273150-HA) was located nucleus, while expression level RH285190-HA, RH260870-HA, RH248450-HA undetectable. No significant differences detected between seven RHΔzfp strains (RHΔ285190, RHΔ248270, RHΔ260870, RHΔ255310, RHΔ309200, RHΔ248450, RHΔ236640) wild-type (WT) strain T. cycle, including plaque formation, invasion, intracellular replication, egress, as well vitro virulence (p > 0.05). However, RHΔ273150 exhibited significantly lower replication efficiency compared other WT strain, vivo mice not affected. Comparative analysis zfp indicates certain may have essential sexual reproductive stage gondii. Taken together, these findings expand our current understanding roles

Язык: Английский

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Identification, characterization and functional analysis of gonadal long noncoding RNAs in a protogynous hermaphroditic teleost fish, the ricefield eel (Monopterus albus)

BMC Genomics, Год журнала: 2022, Номер 23(1)

Опубликована: Июнь 20, 2022

An increasing number of long noncoding RNAs (lncRNAs) have been found to play important roles in sex differentiation and gonad development by regulating gene expression at the epigenetic, transcriptional posttranscriptional levels. The ricefield eel, Monopterus albus, is a protogynous hermaphroditic fish that undergoes sequential change from female male. However, lncRNA unclear. Herein, we performed RNA sequencing analyse patterns five different stages M. albus investigate lncRNAs process. A total 12,746 (1503 known 11,243 new lncRNAs) 2901 differentially expressed (DE-lncRNAs) were identified gonads. target genes DE-lncRNAs included foxo1, foxm1, smad3, foxr1, camk4, ar tgfb3, which mainly enriched signalling pathways related gonadal development, such as insulin pathway, MAPK calcium pathway. We selected 5 highly (LOC109952131, LOC109953466, LOC109954337, LOC109954360 LOC109958454) for full length amplification pattern verification. They all higher levels ovaries intersex gonads than testes, exhibited specific time-dependent ovarian tissue incubated with follicle-stimulating hormone (FSH) human chorionic gonadotropin (hCG). results quantitative real-time PCR (qRT-PCR) analysis dual-luciferase assay showed znf207, targeted LOC109958454, was multiple tissues developmental its also inhibited hormones FSH hCG. These provide insights into role especially regarding natural changes fish, will be useful enhancing our understanding hermaphroditism eel (M. albus) other teleosts.

Язык: Английский

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Discovering weak signals of emerging topics with a triple-dimensional framework

Information Processing & Management, Год журнала: 2024, Номер 61(5), С. 103793 - 103793

Опубликована: Май 25, 2024

Язык: Английский

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Zinc-finger CCHC-type containing protein 8 promotes RNA virus replication by suppressing the type-I interferon responses

Journal of Virology, Год журнала: 2024, Номер 98(9)

Опубликована: Авг. 8, 2024

Host cells have evolved an intricate regulatory network to fine tune the type-I interferon responses. However, full picture of this remains be depicted. In study, we found that knock out zinc-finger CCHC-type containing protein 8 (ZCCHC8) impairs replication influenza A virus (IAV), Sendai (Sev), Japanese encephalitis (JEV), and vesicular stomatitis (VSV). Further investigation unveiled ZCCHC8 suppresses responses by targeting factor 3 (IRF3) signaling pathway. Mechanistically, associates with phosphorylated IRF3 disrupts interaction co-activator CREB-binding (CBP). Additionally, direct binding IFN-stimulated response element (ISRE) ISRE-binding IRF3. Our study contributes comprehensive understanding for negative provides valuable insights control multiple viruses from a host-centric perspective.IMPORTANCEThe innate immune serve as initial line defense against invading pathogens harmful substances. Negative regulation plays essential role in avoiding auto-immune diseases over-activated Hence, could provide novel therapeutic viral infections dysfunction. report negatively regulates We illustrate impedes IRF3-CBP association interacting competes ISRE. demonstrates RNA deeper system

Язык: Английский

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ZFP36 ring finger protein like 1 significantly suppresses human coronavirus OC43 replication

PeerJ, Год журнала: 2023, Номер 11, С. e14776 - e14776

Опубликована: Фев. 20, 2023

CCCH-type zinc figure proteins (ZFP) are small cellular that structurally maintained by ions. Zinc ions coordinate the protein structure in a tetrahedral geometry binding to cystine-cystine or cysteines-histidine amino acids. ZFP’s unique enables it interact with wide variety of molecules including RNA; thus, ZFP modulates several processes host immune response and virus replication. ZFPs have shown their antiviral efficacy against DNA RNA viruses. However, role human coronavirus is little explored. We hypothesized ZFP36L1 also suppresses coronavirus. To test our hypothesis, we used OC43 (HCoV) strain study. overexpressed knockdown HCT-8 cells using lentivirus transduction. Wild type, overexpressed, were each infected HCoV-OC43, titer cell line was measured over 96 hours post-infection (p.i.). Our results show HCoV-OC43 replication significantly reduced overexpression while enhanced started producing infectious at 48 p.i. which an earlier timepoint as compared wild -type cells. Wild-type 72 Overall, current study showed suppressed (OC43) production.

Язык: Английский

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Analysis of the mRNA export protein ZC3H11A in HCMV infection and pan-cancer

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Ноя. 15, 2023

Background We have previously reported that human cytomegalovirus (HCMV) infection could promote the progression of glioma. Here we discovered a stress-induced nuclear protein ZC3H11A (ZC3) through high-throughput sequencing after HCMV infection, which has been recently by our research group in regulating mRNA export under stress conditions. And also, thorough analysis ZC3 pan-cancer and omics data are yet to be conducted. Methods The transcriptomes glioma cells were assessed RNA sequencing. level following validated measured qRT-PCR Western-blot. expression information across analyzed visualized R packages. localization was IHC images from HPA. proteomics transcriptomics different cancers extracted CPTAC portal, comparisons conducted with Python script. genetic alteration, survival prognosis, immune infiltration cBioPortal, TCGA, TIMER2 databases. interaction networks revealed STRING, GEPIA2 TCGA. Results Genes processing pathways upregulated validated. also status generally at high levels cancers, although varied among cancer types. tumor localized whereas normal it mainly found cytoplasmic/membranous. However, some types, increase not accompanied significant elevation level. Additionally, indicated elevated primarily linked negative prognosis majority but still depending on Our annotation suggested ZC3-related proteins involved clusters. Conclusion demonstrated significantly impacted gliomas. Furthermore, identified set genes exhibiting analogous patterns TCGA cohorts, implying potential functional role for processing. study provided valuable insights into new progression. These results lay foundation next regulatory mechanism virus-infected tumors.

Язык: Английский

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