Structure and host specificity of Staphylococcus epidermidis bacteriophage Andhra

Science Advances, Год журнала: 2022, Номер 8(48)

Опубликована: Ноя. 30, 2022

Staphylococcus epidermidis is an opportunistic pathogen of the human skin, often associated with infections implanted medical devices. Staphylococcal picoviruses are a group strictly lytic, short-tailed bacteriophages compact genomes that attractive candidates for therapeutic use. Here, we report structure complete virion S. –infecting phage Andhra, determined using high-resolution cryo–electron microscopy, allowing atomic modeling 11 capsid and tail proteins. The T = 4 icosahedron containing unique stabilizing lining protein. includes 12 trimers receptor binding protein (RBP), lytic also serves to anchor RBPs stem, hexameric knob acts as gatekeeper DNA ejection. Using prediction AlphaFold, identified two proteins comprise tip heterooctamer. Our findings elucidate critical features assembly, host recognition, penetration.

Язык: Английский

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Characterization of Twelve Erwinia amylovora Bacteriophages

Microbiology, Год журнала: 2024, Номер 93(5), С. 666 - 685

Опубликована: Сен. 30, 2024

Язык: Английский

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Prophage transduction promotes the transmission of phage resistance interfering with adsorption among Chinese foodborne Staphylococcus aureus

International Journal of Food Microbiology, Год журнала: 2025, Номер 440, С. 111271 - 111271

Опубликована: Май 16, 2025

Язык: Английский

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Isolation, Characterization, and Preliminary Application of Staphylococcal Bacteriophages in Sichuan Paocai Fermentation

Microorganisms, Год журнала: 2025, Номер 13(6), С. 1273 - 1273

Опубликована: Май 30, 2025

Sichuan paocai, a microbial food predominantly fermented by lactic acid bacteria and hosting complex diverse ecosystem, serves as an ideal habitat for bacteriophages. However, relatively few studies have been conducted on isolating bacteriophages from vegetables their application in vegetable fermentation. In this study, three staphylococcal bacteriophages, ΦSx-2, ΦSs-1, ΦSs-2, were isolated purified paocai using the spot test method. The morphological features of phages characterized transmission electron microscopy (TEM), while key biological properties such one-step growth kinetics systematically evaluated, ultimately verifying taxonomic placement within Caudoviricetes class. Furthermore, potential effects these community structure physicochemical during fermentation investigated high-throughput sequencing standard assays. Microbial analysis demonstrated that introducing significantly increased relative abundance reducing prevalence spoilage Erwinia, Pantoea, Enterobacter. Physicochemical assessments revealed adding accelerated acidification process effectively reduced nitrite levels, concentrations acetic acids. Additionally, notable differences color flavor observed between two groups process. summary, inoculation ΦSs-2 optimized structure, enhanced process, improved quality paocai.

Язык: Английский

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Bacteriophage infection drives loss of β-lactam resistance in methicillin-resistant Staphylococcus aureus

Опубликована: Июнь 2, 2025

Abstract Bacteriophage (phage) therapy is a promising means to combat drug-resistant bacterial pathogens. Infection by phage can select for mutations in populations that confer resistance against infection. However, yield evolutionary trade-offs of biomedical relevance. Here we report the discovery infection certain staphylococcal phages sensitizes different strains methicillin-resistant Staphylococcus aureus (MRSA) β-lactams, class antibiotics which MRSA typically resistant. cells survive these display significant reductions minimal inhibitory concentration β-lactams compared uninfected bacteria. Transcriptomic profiling reveals evolved possess highly modulated transcriptional profiles, where numerous genes involved S. virulence were downregulated. Phage-treated exhibited attenuated phenotypes form reduced hemolysis and clumping. Despite sharing similar phenotypes, whole-sequencing analysis revealed unique genetic profiles during These results suggest complex trajectories predation open up new possibilities reduce drug infections.

Язык: Английский

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Wild-type Lactococcus lactis producing bacteriocin-like prophage lysins

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Июль 14, 2023

Lactococcus is a genus of lactic acid bacteria used in the dairy industry as starter. Lactococci have been found to produce altogether more than 40 different bacteriocins, ribosomally synthesized antimicrobial proteins. All known spp. bacteriocins belong classes I and II, which are mainly heat-resistant peptides. No class III bigger heat-sensitive proteins, including phage tail-like from Unlike prophage lysins not regarded possibly because contribute autolysis, degrading host's own cell wall.Wild-type lactis strain LAC460, isolated spontaneously fermented idli batter, was examined for its activity. We sequenced genome, searched culture supernatant, created knock-out mutants find out source activity.The LAC460 shown kill other strains with protease- lytic Three were identified supernatant. The genes encoding three localized regions chromosome. By mutants, two lysins, namely LysL LysP, demonstrated be responsible resistant action consequence, could behave like targeting killing closely related bacteria. Hence, similar lysin-like novel type bacteriocins.

Язык: Английский

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Isolation and in vitro characterization of novel S. epidermidis phages for therapeutic applications

Frontiers in Cellular and Infection Microbiology, Год журнала: 2023, Номер 13

Опубликована: Май 24, 2023

S. epidermidis is an important opportunistic pathogen causing chronic prosthetic joint infections associated with biofilm growth. Increased tolerance to antibiotic therapy often requires prolonged treatment or revision surgery. Phage currently used as compassionate use and continues be evaluated for its viability adjunctive alternative caused by prevent relapses. In the present study, we report isolation in vitro characterization of three novel lytic phages. Their genome content analysis indicated absence resistance genes virulence factors. Detailed investigation phage preparation any prophage-related contamination demonstrated importance selecting appropriate hosts development from outset. The isolated phages infect a high proportion clinically relevant strains several other coagulase-negative species growing both planktonic culture biofilm. Clinical differing their phenotype profile were selected further identify possible mechanisms behind increased

Язык: Английский

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The effect of phage-antibiotic combination strategy on multidrug-resistant Acinetobacter baumannii biofilms

Journal of Microbiological Methods, Год журнала: 2023, Номер 210, С. 106752 - 106752

Опубликована: Июнь 1, 2023

Язык: Английский

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Characterization and genomic analysis of novel bacteriophage NK20 to revert colistin resistance and combat pandrug-resistant Klebsiella pneumoniae in a rat respiratory infection model

Life Sciences, Год журнала: 2023, Номер 322, С. 121639 - 121639

Опубликована: Март 30, 2023

Язык: Английский

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A mechanism-based pathway toward administering highly active N-phage cocktails

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Ноя. 15, 2023

Bacteriophage (phage) therapy is being explored as a possible response to the antimicrobial resistance public health emergency. Administering mixture of different phage types cocktail one proposed strategy for therapeutic applications, but optimal method formulating cocktails remains major challenge. Each strain has complex pharmacokinetic/pharmacodynamic (PK/PD) properties which depend on nano-scale size, target-mediated, self-dosing nature each strain, and rapid selection resistant subpopulations. The objective this study was explore pharmacodynamics (PD) three unique clinically relevant anti- Pseudomonas phages after simulation dynamic dosing strategies. Hollow Fiber Infection Model (HFIM) an in vitro system that mimics vivo pharmacokinetics (PK) with high fidelity, providing opportunity quantify bacteria concentration profiles over clinical time scales rich sampling. Exogenous monotherapy-bolus (producing max concentrations C = 7 log 10 PFU/mL) regimens LUZ19, PYO2, E215 produced aeruginosa nadirs 0, 2.14, or 2.99 CFU/mL 6 h treatment, respectively. combination bolus (LUZ19 + PYO2 LUZ19 E215) resulted bacterial reduction <2 CFU/mL. In contrast, monotherapy continuous infusion steady-state ss,avg 2 less effective at reducing densities. Specifically, failed reduce density. Next, mechanism-based mathematical model developed describe pharmacodynamics, phage–phage competition, phage-dependent adaptive resistance. Monte Carlo simulations supported dose regimens, predicting lower counts compared prolonged infusions. Together, silico evaluation course will better guide patterns administration individual cocktail.

Язык: Английский

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