Correlation of patient symptoms with SARS-CoV-2 Omicron variant viral loads in nasopharyngeal and saliva samples and their influence on the performance of rapid antigen testing

Microbiology Spectrum, Год журнала: 2024, Номер 12(11)

Опубликована: Окт. 9, 2024

ABSTRACT Evaluating SARS-CoV-2 viral loads in nasopharyngeal (NP) and saliva samples, factors affecting loads, the performance of rapid antigen testing (RAT) have not been comprehensively conducted during Omicron epidemic. This prospective study included outpatients enrolled variant period Japan. Paired NP swab samples were collected to measure by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The correlation between clinical symptoms was examined. an immunochromatography-based RAT kit also assessed. A total 153 patients tested within 3 days symptom onset included. mean load 5.60 log 10 copies/test 3.65 respectively, resulting a significant difference ( P < 0.0001). Fever over 37°C (axillary temperature) number other than fever identified as independent positively correlated with both samples. sensitivity using 92% 68%, positive RT-qPCR results reference. significantly higher ≥37°C and/or at least one those <37°C no (97% vs 83% swabs; 80% 50% saliva). Distinct symptoms, including ≥37°C, may reflect high loads. Rapid testing, only swabs but saliva, would be useful for COVID-19 diagnosis point-of-care particularly symptomatic patients. IMPORTANCE We examined salivary from infection epidemic Japan explored outpatient In addition, we evaluated authorized RT-PCR Intriguingly, observed based on visit. influenced Nevertheless, is considered diagnosis. distinct high-grade fever, could practical diagnostic tool because estimated After epidemic, mild become main focus treatment. Our provides valuable information regarding these

Язык: Английский

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Rapid Antigen Testing

IntechOpen eBooks, Год журнала: 2023, Номер unknown

Опубликована: Июль 6, 2023

Rapid antigen tests became popular during the COVID-19 pandemic as affordable diagnostic tools to help control spread of infection, thanks their cost-effectiveness and simplicity use. These features enabled widespread employment at point-of-care, even in those countries where access care is still limited. The advantages this approach have been demonstrated practical applications envisaged utilization other fields which prevention beneficial, such fight against neglected diseases cancer. As such, need further improve performance rapid assays urgent. At same time, replacement natural bioligands with synthetic ones increase sustainability likely support future development technique. boundaries are expanding towards several fields, beyond clinical diagnostics food safety, forensics, veterinary testing. This book provides a comprehensive overview testing, discussing both its benefits limitations.

Язык: Английский

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The total testing process harmonization: the case study of SARS-CoV-2 serological tests

Clinical Chemistry and Laboratory Medicine (CCLM), Год журнала: 2023, Номер 61(12), С. 2084 - 2093

Опубликована: Авг. 4, 2023

The total testing process harmonization is central to laboratory medicine, leading the test's effectiveness. In this opinion paper five phases of TTP are analyzed, describing, and summarizing critical issues that emerged in each phase with SARS-CoV-2 serological tests have affected their Testing screening population was essential for defining seropositivity and, thus, driving public health policies management COVID-19 pandemic. However, many differences terminology, unit measurement, reference ranges parameters interpreting results make analytical difficult compare, general confusion affects or completely precludes comparability data. Starting from these considerations related tests, through interdisciplinary work, authors highlighted most points formulated proposals effective, positively impacting diagnostic effectiveness tests.

Язык: Английский

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SARS-CoV-2 Rapid Antigen Test Based on a New Anti-Nucleocapsid Protein Monoclonal Antibody: Development and Real-Time Validation

Microorganisms, Год журнала: 2023, Номер 11(10), С. 2422 - 2422

Опубликована: Сен. 28, 2023

SARS-CoV-2 diagnostic tests have become an important tool for pandemic control. Among the alternatives COVID-19 diagnosis, antigen rapid (Ag-RDT) are very convenient and widely used. However, as variants may continuously emerge, replacement of reagents be required to maintain sensitivity Ag-RDTs. Here, we describe development validation Ag-RDT during outbreak Omicron variant, including characterization a new monoclonal antibody (anti-DTC-N 1B3 mAb) that recognizes Nucleocapsid protein (N). The anti-DTC-N mAb recognized sequence TFPPTEPKKDKKK located at C-terminus N main concern. Accordingly, prototypes using mAB detected all variants—Wuhan, Alpha, Gamma, Delta, P2 Omicron. performance best prototype (sensitivity 95.2% samples with Ct ≤ 25; specificity 98.3% overall accuracy 85.0%) met WHO recommendations. Moreover, results from patients’ follow-up study indicated that, if performed within first three days after onset symptoms, displayed 100% sensitivity. Thus, developed herein constitute alternative tools point-of-care diagnosis epidemiological surveillance.

Язык: Английский

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Vaccine-hesitant individuals accumulate additional COVID-19 risk due to divergent perception and behaviors related to SARS-CoV-2 testing – a population based, cross-sectional study

Research Square (Research Square), Год журнала: 2022, Номер unknown

Опубликована: Сен. 13, 2022

Abstract Purpose To investigate the perception of SARS-CoV-2 detection methods, information sources, and opinions on appropriate behavior after receiving negative or positive test results. Methods In a questionnaire-based, cross-sectional study conducted between September 1 November 17, 2021, epidemiological, behavioral, COVID-19-related data was acquired from public in Munich, Germany. Results Most 1,388 participants obtained online media (82.8%) as well state federal authorities (80.3%). 93.4% believed accuracy PCR testing 41.2% rapid antigen tests (RATs). However, RATs were preferred for (59.1%) over (51.1%). 24.0 % all individuals willing to ignore hygiene measures 76.9% less afraid transmission (5.9% 48.8% case RAT). 28.8% reported not self-isolate RAT. Multivariate analyses revealed that non-vaccinated relied governmental ( p =0.0004) more social =0.0216), disbelieved ≤0.0001) while displaying strong preference towards using ≤0.0001), abandon pandemic-related transmitting RAT likely isolate ≤0.0001). Conclusion Insights into sources perception, preferences related are key refining health surveillance campaigns. Non-vaccinated individuals’ divergent believes behaviors possibly increase their COVID-19 risk.

Язык: Английский

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Concordance between GeneXpert and Life Real Detection Kit in the diagnosis of acute respiratory infections

Revista Española de Quimioterapia, Год журнала: 2023, Номер 36(4), С. 427 - 428

Опубликована: Май 2, 2023

Язык: Английский

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Two Years of Experience and Methodology of Korean COVID-19 Living Clinical Practice Guideline Development

Journal of Korean Medical Science, Год журнала: 2023, Номер 38(23)

Опубликована: Янв. 1, 2023

In Korea, during the early phase of coronavirus disease 2019 (COVID-19) pandemic, we responded to uncertainty treatments under various conditions, consistently playing catch up with speed evidence updates. Therefore, there was high demand for national-level evidence-based clinical practice guidelines clinicians in a timely manner. We developed and updated living recommendations through transparent development process multidisciplinary expert collaboration.The National Evidence-based Healthcare Collaborating Agency (NECA) Korean Academy Medical Sciences (KAMS) collaborated develop trustworthy guidelines. The NECA-supported methodological sections 8 professional medical societies KAMS worked experts, 31 were involved annually. total 35 questions, including medications, respiratory/critical care, pediatric emergency diagnostic tests, radiological examinations.An search began March 2021 monthly updates performed. It expanded other areas, interval organized by steering committee owing priority changes. Evidence synthesis recommendation review performed researchers, within 3-4 months.We provided on schemes disseminated them public, policymakers stakeholders using webpages social media. Although output successful, some limitations. rigor issues, urgent timelines public dissemination, education new developers, spread several COVID-19 variants have as barriers. must prepare systematic processes funding future pandemics.

Язык: Английский

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None

Revista Española de Quimioterapia, Год журнала: 2023, Номер 36(4)

Опубликована: Май 3, 2023

Efficacy, effectiveness and safety of the adjuvanted influenza vaccine in population aged 65 or over ABSTRACTMost complications deaths related to seasonal flu occur elderly (≥65 years) with comorbidities, is most effective way prevent them.Immunization less older adults due immunosenescence.MF59-adjuvanted vaccines, designed improve magnitude, persistence amplitude immune response people, have been used clinical practice since 1997 their trivalent formulation and, 2020, tetravalent formulation.Data from various studies show that these vaccines are not only safe for all age groups, a reactogenicity profile similar conventional vaccine, but also they especially boosting by increasing antibody titers after vaccination significantly reducing risk hospital admission.Adjuvanted shown provide cross-protection against heterologous strains be as high-dose over.In this review, scientific evidence on efficacy MF59-adjuvanted real people ≥65 years analyzed through narrative descriptive review literature data trials, observational systematic reviews meta-analysis.

Язык: Английский

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Using Large Scale Rapid Antigen Testing (RAT) to Inform Participatory Ad-Hoc Community Surveillance for Emerging Communicable Disease Epidemics

IntechOpen eBooks, Год журнала: 2023, Номер unknown

Опубликована: Авг. 4, 2023

Besides the diagnostic use for infectious diseases in a point-of-care clinical settings, simplicity and ease of self-performed RAT can also be an alternative approach informing disease surveillance at community level, carrying potential advantage enhanced timeliness, acceptability, flexibility. Commissioned by Hong Kong Government during catastrophic fifth wave COVID-19 pandemic, our team established maintained ad-hoc large-scale participatory daily antigen rapid testing (DARTS) system real-time situational awareness SARS-CoV-2 activity to inform policy consideration timely manner. This Chapter will describe concept design approach, examine practical feasibility challenges, related logistical on implementation maintenance, technical aspects data analysis cater unique need, other additional contribution understanding novel (estimating vaccine effectiveness, symptomatology viral shedding pattern).

Язык: Английский

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