Network Analysis for Uncovering the Relationship between Host Response and Clinical Factors to Virus Pathogen: Lessons from SARS-CoV-2

Viruses, Год журнала: 2022, Номер 14(11), С. 2422 - 2422

Опубликована: Окт. 31, 2022

Analysing complex datasets while maintaining the interpretability and explainability of outcomes for clinicians patients is challenging, not only in viral infections. These often include a variety heterogeneous clinical, demographic, laboratory, personal data, it single factor but combination multiple factors that contribute to patient characterisation host response. Therefore, multivariate approaches are needed analyse these datasets, which impossible with univariate comparisons (e.g., one immune cell subset versus clinical factor). Using SARS-CoV-2 infection as an example, we employed similarity network (PSN) approach assess relationship between course performed visualisation data interpretation. A PSN analysis ~85 immunological (cellular humoral) ~70 250 recruited coronavirus disease (COVID-19) who were sampled four eight weeks after PCR-confirmed identified minimal signature, well laboratory strongly associated severity. Our study demonstrates benefits implementing identify relevant visualise their relationships infection, model generally applicable any dataset.

Язык: Английский

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Macrophage-Derived Chemokine MDC/CCL22: An Ambiguous Finding in COVID-19

Опубликована: Май 6, 2023

Macrophage-derived chemokine belongs to the CC subfamily. It is produced by dendritic cells (DCs) and macrophages with or without external stimulation. We have previously shown a statistically significant depletion of MDC/CCL22 concentrations in number studies concerning COVID-19. These shifts demonstrated stability unrelated SARS-CoV-2 genetic variant remained noticeable even convalescent patients. In this work, we analyze dynamics various diseases, including those that manifest inflammation lung tissue. addition, provide our hypothesis on such decrease If its secretion producer unperturbed, then it possible for viral products bind block functional activity. There is, however, another explanation directly linked DC subpopulations inhibition their function. also discuss MDC/CCL22's role immunology novel coronavirus infection, based both own data other studies.

Язык: Английский

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Main circulating CD8⁺ T cell subsets in patients with systemic lupus erythematosus

Rheumatology Science and Practice, Год журнала: 2024, Номер 62(1), С. 90 - 97

Опубликована: Март 1, 2024

Relevance . Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by loss of immune tolerance and sustained production autoantibodies. The aim the study – to compare composition peripheral blood cytotoxic CD8 + T lymphocytes (Tc) subsets assess clinical significance them in systemic erythematosus. Materials methods. A total 35 SLE patients 49 healthy volunteers were included study. Phenotyping cell subpopulations was carried out means flow cytometry. determined using CD3 , CD4 antibodies. Tc identified CD45RA CD62L Also expression chemokine receptors (CCR4, CCR6, CXCR3 CXCR5) on cells assessed main determined: Type 1 (Tc1), type 2 (Tc2), 17 (Tc17), 17/1 (Tc17.1), 17/22 (Tc17.22) follicular (Tfc). Results absolute relative number significantly higher group with compared control group. Additionally, there a significant decrease Tc1, 17.1 Tfc1 increase Tc2, Tfc Tfc17.1 within when There positive correlationfor Tc1 levels C3 C4 complement components (r=0.404, p<0.05). Conclusions altered It found that contained increased Tc2 cells, which seems be associated markers activity. These results demonstrate prominent pathological role SLE. While Tc17, Tc17.1, probably have regulatory functions

Язык: Английский

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Effect of Immunity to SARS-CoV-2 Virus on Blood Cellular Composition

Epidemiology and Vaccinal Prevention, Год журнала: 2024, Номер 23(2), С. 50 - 60

Опубликована: Май 3, 2024

Relevance. The new coronavirus infection (COVID-19) is still a public health problem and threat to socio-economic well-being. Most studies have focused predominantly on humoral immunity, there are no data the cellular composition of blood in dynamics. Aim. To study dynamics changes depending type immunity formed (natural, hybrid, breakthrough, postvaccinal) SARS-CoV-2 virus. Materials Methods. A total 130 volunteers participated study. Immunophenotyping peripheral leukocytes using flow cytometry was performed. presence specific IgG antibodies N-protein SARS-CoV-2, IgA cytokines (IL-4, IL-10, IFN-γ, TNF-α) assessed serum by ELISA. Results Discussion. statistically significant increase BL recorded with hybrid 1 month (14,0% (12,3–16,4%)) after vaccination compared healthy (9,1% (6,4–10,2%), p = 0,0007) people primary COVID-19 (10,2% (8,3–12,1%), 0,0134). In natural as well revaccinated people, an B1-cells (CD3-CD19+CD5+CD27-) observed during 3–9 months observation. It shown that B-lymphocytes «switched» class synthesized detected breakthrough immunity. Increased levels T-lymphocytes expressing HLA-DR were all individuals 6–9 follow-up. Volunteers showed positivity index when assessing Conclusions. Vaccination promotes protective sufficient for timely activation memory T- B-cells maintenance immunologic efficacy against COVID-19. results help assess strain innate adaptive novel fill gaps understanding immunopathogenesis

Язык: Английский

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Role of IL-27 in COVID-19: A Thin Line between Protection and Disease Promotion

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(14), С. 7953 - 7953

Опубликована: Июль 21, 2024

Cytokine storm is usually described as one of the main reasons behind COVID-associated mortality. Cytokines are essential protein molecules engaged in immune responses; they play a critical role protection against infections. However, also contribute to inflammatory reactions and tissue damage, becoming double-edged sword context COVID-19. Recent studies have suggested various cytokines chemokines that crucial response SARS-CoV-2 infection. One such cytokine interleukin 27 (IL-27), which has been found be elevated blood plasma patients with Within this study, we will explore IL-27 responses analyze both existing literature our own prior research findings on It affects wide variety cells. Regardless pathological process it involved in, for upholding necessary balance between damage cytotoxicity infectious agents and/or tumors. In COVID-19, multiple processes, including antiviral via CD8+ cells, IgG subclass switching, even activation Tregs.

Язык: Английский

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