Influenza A Virus Subtype H9N2 Infection Induces Respiratory Microbiota Dysbiosis in Chickens via Type-I Interferon-Mediated Mechanisms
FEMS Microbes,
Год журнала:
2025,
Номер
6
Опубликована: Янв. 1, 2025
Abstract
Avian
influenza
virus
(AIV)
poses
significant
threats
to
poultry
and
human
health.
This
study
investigates
the
impact
of
H9N2
AIV
infection
on
respiratory
microbiota
chickens
using
16S
rRNA
gene
sequencing.
Total
48
one-day-old
specific
pathogen-free
were
assigned
six
groups:
a
control
five
post-infection
groups
(days
1,
3,
5,
7,
9).
After
15-day
stabilization
period,
infected
received
viral
inoculum
(107
TCID50/ml)
via
ocular,
intra-nasal,
intra-tracheal
routes.
Tracheal
broncho-alveolar
lavage
samples
analyzed.
Significant
reductions
in
diversity
observed
days
9
post-infection,
compared
d0
controls.
Permutational
Multivariate
Analysis
Variance
confirmed
beta
differences
(P
=
0.001)
between
uninfected
groups.
The
microbial
shifts
from
d5
d9
marked
by
increased
Proteobacteria,
decreased
Actinobacteria
Firmicutes,
rise
Dickeya.
Elevated
type-I
interferon
(IFN-β)
viperin
expression
at
coincided
with
reduced
diversity,
highlighting
microbiota’s
role
modulating
host
responses
suggesting
potential
biomarkers
for
dysbiosis.
Язык: Английский
Current Status of Poultry Recombinant Virus Vector Vaccine Development
Vaccines,
Год журнала:
2024,
Номер
12(6), С. 630 - 630
Опубликована: Июнь 6, 2024
Inactivated
and
live
attenuated
vaccines
are
the
mainstays
of
preventing
viral
poultry
diseases.
However,
development
recombinant
DNA
technology
in
recent
years
has
enabled
generation
virus
vector
vaccines,
which
have
advantages
multiple
diseases
simultaneously
simplifying
vaccination
schedule.
More
importantly,
some
can
induce
a
protective
immune
response
presence
maternal
antibodies
offer
long-term
protection.
These
compensate
for
shortcomings
traditional
vaccines.
This
review
describes
construction
characterization
primarily
vaccine
vectors,
including
fowl
poxvirus
(FPV),
adenovirus
(FAdV),
Newcastle
disease
(NDV),
Marek’s
(MDV),
herpesvirus
turkey
(HVT).
In
addition,
pathogens
targeted
immunoprotective
effect
different
also
presented.
Finally,
this
discusses
challenges
developing
proposes
strategies
improving
efficacy.
Язык: Английский
In vivo overexpression of the avian interleukin-17 in a necrotic enteritis disease model modulates the expression of antimicrobial peptides in the small intestine of broilers
Cytokine,
Год журнала:
2024,
Номер
183, С. 156749 - 156749
Опубликована: Сен. 4, 2024
Язык: Английский
Activation of Antiviral Host Responses against Avian Influenza Virus and Remodeling of Gut Microbiota by rLAB Vector Expressing rIL-17A in Chickens
ACS Infectious Diseases,
Год журнала:
2024,
Номер
10(8), С. 3026 - 3041
Опубликована: Июль 6, 2024
Low-pathogenic
avian
influenza
virus
(LPAIV)
remains
the
most
common
subtype
of
type-A
that
causes
moderate
to
severe
infection
in
poultry
with
significant
zoonotic
and
pandemic
potential.
Due
high
mutability,
increasing
drug
resistance,
limited
vaccine
availability,
conventional
means
prevent
intra-
or
interspecies
transmission
AIV
is
highly
challenging.
As
an
alternative
control
infections,
cytokine-based
approaches
augment
antiviral
host
defense
have
gained
attention.
However,
selective
application
cytokines
critical
since
unregulated
expression
cytokines,
particularly
proinflammatory
ones,
can
cause
substantial
tissue
damage
during
acute
phases
immune
responses.
Moreover,
depending
on
type
cytokine
its
impact
intestinal
microbiota,
outcomes
cytokine-gut
microflora
interaction
a
effect
overall
against
infections.
Our
recent
study
demonstrated
some
prominent
roles
chicken
IL-17A
(ChIL-17A)
regulating
responses
infection,
however,
vitro
model.
For
more
detailed
insights
into
ChIL-17A
function,
present
study,
we
investigated
whether
ChIL-17A-meditated
elevated
translate
effective
protection
vivo
system.
considering
role
gut
health
fostering
innate
local
responses,
further
studied
contributory
relationships
between
microbiota
immunity
chickens.
this,
employed
recombinant
lactic
acid-producing
bacterial
(LAB)
vector,
Lactococcus
lactis,
expressing
analyzed
functionality
chickens
LPAIV
(A/H9N2)
infection.
delineates
mucosal
delivery
rL.
lactis
triggers
signaling
cascades
drive
positive
shift
phylum
Firmicutes,
along
marked
decline
Actinobacteriota
Proteobacteria,
favoring
We
propose
ChIL-17A-mediated
expansion
beneficial
might
form
healthy
microbial
community
augments
infections
Язык: Английский