Castanet: a pipeline for rapid analysis of targeted multi-pathogen genomic data DOI
Richard Mayne,

Shannah Secret,

Cyndi G. Geoghegan

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 2, 2024

Abstract Motivation Target enrichment strategies generate genomic data from multiple pathogens in a single process, greatly improving sensitivity over metagenomic sequencing and enabling cost-effective, high throughput surveillance clinical applications. However, uptake by research laboratories is constrained an absence of computational tools that are specifically designed for the analysis multi-pathogen sequence data. Here we present Castanet pipeline: pipeline end-to-end processing consensus generation use with to work short-read produced existing targeted strategies, but can be readily deployed on any BAM file generated another methodology. It packaged usability features, including graphical interface installer script. Results In addition genome reconstruction, reports method-specific metrics enable quantification capture efficiency, estimation pathogen load, differentiation low-level positives contamination, assessment quality. used as traditional generation, its strength lies ability process flexible, pre-defined set interest directly experiments. our tests, sequences were accurate reconstructions reference sequences, instances where strains same present. performs effectively standard laptop computers entire output 96-sample run (50M reads) using batch command, < 2 h. Availability Implementation Source code freely available under GPL-3 license at https://github.com/MultipathogenGenomics/castanet , implemented Python 3.10 supported Ubuntu Linux 22.04 other Bash-like environments. The this study have been deposited European Nucleotide Archive (ENA) EMBL-EBI accession number PRJEB77004.

Язык: Английский

Strengthening Global Health Resilience: Marburg Virus-Like Particle Vaccines and the One Health Approach DOI Creative Commons
Ram Bahadur Khadka, Khimdhoj Karki, Jitendra Kumar Pandey

и другие.

Science in One Health, Год журнала: 2024, Номер 3, С. 100076 - 100076

Опубликована: Янв. 1, 2024

The Marburg virus (MARV), belonging to the

Язык: Английский

Процитировано

6

Genomic characterization uncovers transmission dynamics of Marburg Virus in Rwanda following a single zoonotic spillover event DOI Open Access
Yvan Butera, Léon Mutesa, Edyth Parker

и другие.

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 5, 2024

Abstract The ongoing outbreak of Marburg virus disease (MVD) in Rwanda marks the third largest historically, though it has exhibited lowest fatality rate. Genomic analysis identified a lineage with limited internal diversity most closely related to genome sequence from sporadic case sampled 2014 Uganda, lineages have diverged common ancestor that was circulating for decades animal reservoir. Notably, data also provide evidence resulted single zoonotic transmission event human-to-human transmission, rather than multiple independent events. Rwandan MVD prompted thorough investigation included contact tracing, clinical assessment, travel history, sequencing, and serology testing, trace virus’s origin. Results investigations linked index mining cave inhabited by Rousettus aegyptiacus (the Egyptian fruit bat), where three individuals tested seropositive IgG IgM, further supporting origin through human-animal interactions.

Язык: Английский

Процитировано

4

Genomic and transmission dynamics of the 2024 Marburg Virus Outbreak in Rwanda DOI Creative Commons
Yvan Butera, Léon Mutesa, Edyth Parker

и другие.

Nature Medicine, Год журнала: 2024, Номер unknown

Опубликована: Дек. 16, 2024

Язык: Английский

Процитировано

2

Genomic Evolution and Phylodynamics of the Species Orthomarburgvirus marburgense (Marburg and Ravn Viruses) to Understand Viral Adaptation and Marburg Virus Disease’s Transmission Dynamics DOI Creative Commons
Claude Mambo Muvunyi, Nouh Saad Mohamed, Emmanuel Edwar Siddig

и другие.

Pathogens, Год журнала: 2024, Номер 13(12), С. 1107 - 1107

Опубликована: Дек. 14, 2024

In this review, we investigated the genetic diversity and evolutionary dynamics of Orthomarburgvirus marburgense species that includes both Marburg virus (MARV) Ravn (RAVV). Using sequence data from natural reservoir hosts human cases reported during outbreaks, conducted comprehensive analyses to explore variability, constructing haplotype networks at genome gene levels elucidate viral pathways. Our results revealed distinct trajectories for MARV RAVV, with exhibiting higher adaptability across different ecological regions. showed substantial evidence varied pressures, suggesting an ability adapt diverse environments. contrast, RAVV demonstrated limited diversity, no detected recombination events, stability. These differences indicate that, while continues diversify regions, may be constrained in its potential, possibly reflecting differing roles within ecology species. analysis explains mechanisms these viruses, highlighting is going through adaptation human-to-human transmission, alarmingly underscoring global concern about causing next pandemic. However, further transdisciplinary One Health research warranted answer some remaining questions including host range susceptibility domestic wildlife as well role biodiversity network disease’s dynamics.

Язык: Английский

Процитировано

2

Castanet: a pipeline for rapid analysis of targeted multi-pathogen genomic data DOI
Richard Mayne,

Shannah Secret,

Cyndi G. Geoghegan

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 2, 2024

Abstract Motivation Target enrichment strategies generate genomic data from multiple pathogens in a single process, greatly improving sensitivity over metagenomic sequencing and enabling cost-effective, high throughput surveillance clinical applications. However, uptake by research laboratories is constrained an absence of computational tools that are specifically designed for the analysis multi-pathogen sequence data. Here we present Castanet pipeline: pipeline end-to-end processing consensus generation use with to work short-read produced existing targeted strategies, but can be readily deployed on any BAM file generated another methodology. It packaged usability features, including graphical interface installer script. Results In addition genome reconstruction, reports method-specific metrics enable quantification capture efficiency, estimation pathogen load, differentiation low-level positives contamination, assessment quality. used as traditional generation, its strength lies ability process flexible, pre-defined set interest directly experiments. our tests, sequences were accurate reconstructions reference sequences, instances where strains same present. performs effectively standard laptop computers entire output 96-sample run (50M reads) using batch command, < 2 h. Availability Implementation Source code freely available under GPL-3 license at https://github.com/MultipathogenGenomics/castanet , implemented Python 3.10 supported Ubuntu Linux 22.04 other Bash-like environments. The this study have been deposited European Nucleotide Archive (ENA) EMBL-EBI accession number PRJEB77004.

Язык: Английский

Процитировано

0