Development of a Recombinant Omicron BA.1 Subunit Vaccine Candidate in Pichia pastoris

Microbial Biotechnology, Год журнала: 2025, Номер 18(1)

Опубликована: Янв. 1, 2025

ABSTRACT Low‐cost and safe vaccines are needed to fill the vaccine inequity gap for future pandemics. Pichia pastoris is an ideal expression system recombinant protein production due its cost‐effective easy‐to‐scale‐up process. Here, we developed a next‐generation SARS‐CoV2 Omicron BA.1‐based candidate expressed in P. . The receptor binding domain of BA.1 spike (RBD‐Omicron) was produced at 0.35 g/L supernatant. With 60% recovery after two‐step purification, RBD‐Omicron showed 99% purity. After vitro characterisation purified via chromatography, mass spectrometry, calorimetry surface plasmon resonance‐based methods, it injected into mice immunization studies. Three different doses Alum CpG adjuvanted were investigated 10 μg gave highest antigenicity. two vaccination, IgG titers serum reached more than 6 These antibodies also recognized earlier (Delta Plus: B.1.617.2) later (Eris: EG.5, Pirola: BA.2.86) variants. long‐term immunological response measured by analyzing antibody T‐cell splenocytes 60 weeks. Interestingly, Th1 significantly high even year. subvariants dominantly circulating world, so sub‐lineage‐based can be used RBD‐Omicron‐based this study suitable technology transfer transition clinic.

Язык: Английский

---

Infectious diseases in Poland in 2021

Przeglad Epidemiologiczny, Год журнала: 2024, Номер 77(4), С. 411 - 428

Опубликована: Май 20, 2024

This article aims at evaluating the epidemiological situation of infectious and parasitic diseases in Poland 2013.This was mainly based on statistical data derived from annual bulletins "Infectious poisonings 2013" "Vaccinations (NIPH-NIH, CSI, Warsaw 2014) specified particular articles chronicle current issue Epidemiological Review. Data fatal cases due to registered 2013 previous years were obtained Demographic Surveys Labour Market Department Central Statistical Office.As with years, upper respiratory tract infections classified as "influenza influenza-like illness" most prevalent a total number amounting 3,164,405 (8,218.7/100,000). Compared 1,460,037 (3,789.0/100,000) 2012 median 2007-2011, it an increase incidence by 116.9% 469.5%, respectively. In 2013, decreasing tendency bacterial intestinal sustained. Out infections, those caused coccobacilli Salmonella spp. still predominant. 7 578 (19.7/100,000) such reported. decrease 10.0% 21.9%, A 42 699 (110.9/100,000) viral this group, common rotaviruses--23,529 (61.1/100,000). Having referred 2012, decreased ca 0.6%, while comparison 5.5%. diarrhoea unspecified origin children under 2 old increased 28.4%. 2,183 pertussis (5.7/100,000) registered, which compared year indicated 53%. mumps 12.3% noted. year, there more than 5-fold rubella incidence. 6,263 (16.3/100,000), 2013--38,548 (100.1/100,000). Considerable resulted compensatory epidemic affected boys who not inoculated MMR vaccine. congenital rubella. 84 (0.22/100,000) measles invasive disease H. influenzae 25 (0.06/100,000). 30.5% 19.3%, The Streptococcus pneumoniae etiology amounted 540. 22.5% 95.3%, considered sepsis pathogen 32.9%. tuberculosis (all manifestations) 19.6/100,000 18.8, 18.2 17.8/100,000. 1,097 HIV (2.85/100,000) did change. total, 36 malaria notified infected abroad visiting endemic areas.

Язык: Английский

---

Safe plant Hsp90 adjuvants elicit an effective immune response against SARS-CoV2-derived RBD antigen

Vaccine, Год журнала: 2024, Номер 42(14), С. 3355 - 3364

Опубликована: Апрель 17, 2024

Язык: Английский

---

Vaccines for Respiratory Viruses—COVID and Beyond

Vaccines, Год журнала: 2024, Номер 12(8), С. 936 - 936

Опубликована: Авг. 22, 2024

The COVID-19 (coronavirus disease 2019) pandemic had an extensive impact on global morbidity and mortality. Several other common respiratory viruses, such as the influenza virus syncytial (RSV), are endemic or epidemic agents causing acute infections that easily transmissible pose a significant threat to communities due efficient person-to-person transmission. These viruses can undergo antigenic variation through genetic mutations, resulting in emergence of novel strains variants, thereby diminishing effectiveness current vaccines, necessitating ongoing monitoring adjustment vaccine antigens. As virus-specific immunity is maintained only for several weeks months after infection, there emergent need develop effective durable vaccines. Additionally, specific populations, elderly immunocompromised individuals, may exhibit reduced immune responses posing challenges vaccines elicit potent immunity. We present comprehensive review molecular mechanisms underlying pathogenesis virulence RSV, virus, severe syndrome coronavirus 2 (SARS-CoV-2). discuss approaches under development. A thorough understanding strategies encountered during development process lead advancement next-generation

Язык: Английский

---

Comparing COVID-19 literacy and vaccine hesitancy among health care workers, including oral health professionals, in Japan

BDJ Open, Год журнала: 2025, Номер 11(1)

Опубликована: Янв. 4, 2025

Язык: Английский

---

Rethinking Optimal Immunogens to Face SARS‐CoV‐2 Evolution Through Vaccination

Influenza and Other Respiratory Viruses, Год журнала: 2025, Номер 19(1)

Опубликована: Янв. 1, 2025

ABSTRACT SARS‐CoV‐2, which originated in China late 2019, quickly fueled the global COVID‐19 pandemic, profoundly impacting health and economy worldwide. A series of vaccines, mostly based on full SARS‐CoV‐2 Spike protein, were rapidly developed, showing excellent humoral cellular responses high efficacy against both symptomatic infection severe disease. However, viral evolution waning neutralizing strongly challenged vaccine long term effectiveness, mainly infection, making necessary a strategy repeated updated booster shots. In this vaccination context, antibody repertoire diversification was evidenced, although immune imprinting after doses or reinfection also demonstrated identified as major determinant immunological to antigen exposures. Considering that small domain receptor binding (RBD), is target antibodies concentrates most mutations, following text aims provide insights into ongoing debate over best strategies for boosters. We address relevance developing new vaccines evolving RBD, thus focusing relevant antigenic sites variants. combination with immunofusing computerized approaches could minimize imprinting, therefore optimizing efficacy.

Язык: Английский

---

Plattformtechnologien in der Impfstoffentwicklung

Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz, Год журнала: 2025, Номер unknown

Опубликована: Март 4, 2025

Zusammenfassung Als Plattformtechnologien im engeren Sinn werden nachfolgend Ansätze der Impfstoffentwicklung bezeichnet, bei denen Impfstoff stets auf einem identischen Grundgerüst basiert und sich nur hinsichtlich des Antigens unterscheidet. Ein Vorteil von besteht in raschen Anpassbarkeit dieser Technologien für die Entwicklung eines Impfstoffs gegen neuartige Erreger oder Varianten. Bei derzeit EU zugelassenen Impfstoffen virale Vektoren mRNA als Plattformen verwendet. dienen dabei rekombinante Adenoviren (Ad), das vesikuläre Stomatitis-Virus (VSV) modifizierte Vacciniavirus Ankara (MVA). Die Applikation mRNA-basierten erfolgt Form Lipidnanopartikeln (LNPs). Funktion LNPs dem Schutz vor Abbau, Förderung Aufnahme Zellen einer Adjuvanswirkung.

---

COVID-19: India’s Economic Crunch and Proposed Advanced Technological Measures to Restrain Future Infections

Reviews of Environmental Contamination and Toxicology, Год журнала: 2025, Номер 263(1)

Опубликована: Март 21, 2025

Язык: Английский

---

Innovation in times of crisis: a pragmatic and inclusive approach to cope with urgent global sustainability challenges

Frontiers in Environmental Economics, Год журнала: 2025, Номер 4

Опубликована: Март 26, 2025

The term “polycrisis” has become a buzzword to describe the entanglement and reinforcement of multiple global crises that may put survival humankind at risk. It builds upon Sustainability Science its research on complex interactions systemic risks. approach roots in “Limits Growth” report published by Club Rome 1972. publication predicted sustainability crisis response growing human resource consumption. threat is real, but there are lessons be learned from coping with past how they were addressed far-sighted government initiatives incentivized decentralized innovation systems achieve well-defined objectives. These proved effective because based an adequate understanding endogenous nature economic development biocapacity societal resilience can enhanced through higher levels complexity. Contemporary European mission-oriented policies (MOIPs) their strong faith state as pacesetter economy lack this understanding. In paper, more pragmatic policy proposed accelerate progress UN Sustainable Development Goals (UN SDGs) general, food security particular. combines target orientation US missions commitment international crop networks mobilize entrepreneurship for inclusive development. Throughout history, such opportunity-driven approaches public responses than attempts minimize risks limiting growth.

Язык: Английский

---

The COVID-19 Vaccination Rollout in Tanzania: The Role of Coordination in Its Success

Vaccines, Год журнала: 2025, Номер 13(5), С. 484 - 484

Опубликована: Апрель 30, 2025

Background: The national rollout of a vaccine is complex and significant undertaking, made more challenging when the health system experiencing shock, such as in pandemic. Tanzania had relative success its COVID-19 vaccination compared to other African countries. Objectives: To better understand factors that contributed this success, we examined role coordination (one six immunization building blocks) on outcomes rollout. Methods: We obtained qualitative information from published literature, program documents for Mainland Zanzibar, reports two documentation workshops with national, regional, district stakeholders government, partners, academia, civil society. Triangulating information, describe structure, roles responsibilities members, changes their engagement activities over 18 months following introduction vaccine. also quantitative data CHANJOCOVID analyze time trends coverage rates period August 2021 December 2022. Results: found multi-level, multi-partner integrated mechanism provided strategic direction, oversight, guidance structure was initially weak but strengthened time. Based level undertaken, identified three periods marking different strengths mechanisms, these corresponded mainland. In first (July-December 2021), weak, low, only 3% target population vaccinated second (January-May 2022), stakeholder expanded improved, there concurrent rise 4% 25%. third (June-December further strengthened, strategies were intensified; corresponding increase uptake observed reaching 100% population. Conclusions: Qualitative insights suggest positive association between strength coverage. Coordination fostered collaboration, enhanced engagement, facilitated data-driven decision making. This enabled overcome challenges achieve progress Strong effective collaboration among are essential mechanisms processes optimize delivery resources ensure equitable distribution vaccines Tanzania.

Язык: Английский

---