Genetic diversity of porcine circoviruses 2 and 3 circulating among wild boars in the Moscow Region of Russia
Frontiers in Veterinary Science,
Год журнала:
2024,
Номер
11
Опубликована: Июнь 26, 2024
Porcine
circoviruses
(PCVs)
are
widely
distributed
in
swine
herds.
PCV2,
the
significant
pathogen,
causes
infections
characterized
by
growth
and
development
disorders,
skin
lesions,
respiratory
distress.
PCV3
has
been
circulating
worldwide
can
be
associated
with
various
clinical
signs
disease
developments.
Wild
boars
main
reservoir
of
these
pathogens
wildlife
create
an
alarming
threat
to
pig
farming.
In
Russia,
three
PCV2
genotypes
(PCV2a,
PCV2b,
PCV2d)
were
identified
farms.
Additionally,
was
observed
herds
during
monitoring
studies
country.
However,
data
considering
circulation
PCVs
wild
Russia
is
scant.
For
this
purpose,
we
performed
PCR
assays
samples
from
30
hunted
Moscow
Region
2021-2023.
The
ratios
positive
for
PCV3,
or
coinfected
50,
10,
13.3%,
respectively.
sequenced
15
four
complete
genomes
conducted
phylogenetic
analysis,
which
divided
isolates
into
two
groups:
PCV2d
PCV2b.
study
showed
a
high
infection
rate
among
boars,
dominance.
Simultaneously,
also
circulates
boars.
obtained
results
provide
basis
preventive
measures
support
transmission
risks
between
farm
animals.
Язык: Английский
Molecular Positivity of Porcine Circovirus Type 2 Associated with Production Practices on Farms in Jalisco, Mexico
Viruses,
Год журнала:
2024,
Номер
16(10), С. 1633 - 1633
Опубликована: Окт. 19, 2024
The
modernization
of
pig
production
has
led
to
increasingly
larger
populations
pigs.
This
dynamic
allows
for
accelerated
and
ensures
a
steady
pork
supply
but
also
facilitates
the
spread
infections.
PCV2
is
ubiquitous
virus
can
cause
PCV2-associated
diseases,
depending
on
practices.
study
aimed
evaluate
conditions
in
state
Jalisco,
Mexico,
correlate
them
with
PCV2.
A
total
4207
serum
samples
from
80
farms
were
analyzed.
Epidemiological
data
collected
used
investigate
factors
associated
detection.
relative
frequency
approximately
30%
was
detected,
primarily
grower
pigs
maintained
multisite
farms.
Several
practices,
particularly
biosecurity
measures,
analyzed
Язык: Английский
First Molecular Characterisation of Porcine Parvovirus 7 (PPV7) in Italy
Viruses,
Год журнала:
2024,
Номер
16(6), С. 932 - 932
Опубликована: Июнь 8, 2024
Porcine
parvoviruses
(PPVs)
are
among
the
most
important
agents
of
reproductive
failure
in
swine
worldwide.
PPVs
comprise
eight
genetically
different
species
ascribed
to
four
genera:
Protoparvovirus
(PPV1,
PPV8),
Tetraparvovirus
(PPV2-3),
Copiparvovirus
(PPV4-6),
and
Chaphamaparvovirus
(PPV7).
In
2016,
PPV7
was
firstly
detected
USA
afterwards
Europe,
Asia,
South
America.
Recently,
it
also
identified
Italy
pig
farms
with
failure.
This
study
aimed
evaluate
circulation
domestic
wild
pigs
Sardinia,
Italy.
addition,
its
coinfection
Circovirus
2
(PCV2)
3
(PCV3)
analysed,
Italian
strains
were
molecularly
characterised.
and,
for
first
time,
The
viral
genome
20.59%
samples.
detection
significantly
lower
pigs,
higher
PCV2/PCV3
co-infection
rates
observed
PPV7-positive
than
PPV7-negative
pigs.
Molecular
characterisation
NS1
gene
showed
a
very
high
frequency
recombination
that
could
presumably
promote
virus
spreading.
Язык: Английский
A Subunit Vaccine Harboring the Fusion Capsid Proteins of Porcine Circovirus Types 2, 3, and 4 Induces Protective Immune Responses in a Mouse Model
Viruses,
Год журнала:
2024,
Номер
16(12), С. 1964 - 1964
Опубликована: Дек. 23, 2024
Coinfections
with
porcine
circovirus
types
2,
3,
and
4
(PCV2,
PCV3,
PCV4)
are
increasingly
being
detected
in
the
swine
industry.
However,
there
is
no
commercially
available
vaccine
which
prevents
coinfection
PCV2,
PCV4.
The
development
of
a
expressing
capsid
(Cap)
fusion
proteins
multiple
PCVs
represents
promising
approach
for
broadly
preventing
infection
PCVs.
In
this
study,
we
developed
PCV
subunit
candidate
(Cap
2-3-4)
by
predicting,
screening,
fusing
antigenic
epitopes
Cap
Immunoprotection
assays
showed
that
prokaryotic
expression
2-3-4
could
effectively
induce
high
levels
PCV4
Cap-specific
antibodies
successfully
neutralize
both
PCV2
PCV3.
Furthermore,
demonstrated
potent
ability
to
activate
cellular
immunity
thus
prevent
lung
damage
mice.
This
study
provides
new
option
broad
vaccines
against
Язык: Английский
Porcine Circovirus Diseases: Current Insights and Future Strategies for Effective Control, with a Focus on Porcine Circovirus 2 (PCV2)
Microbiology Research Journal International,
Год журнала:
2024,
Номер
34(12), С. 283 - 298
Опубликована: Дек. 30, 2024
Porcine
Circovirus
Associated
Disease
(PCVAD),
caused
by
Type
2
(PCV2),
is
a
major
health
concern
in
swine
production,
leading
to
significant
economic
losses.
The
diagnosis
of
PCVAD
relies
on
combination
clinical
signs
and
confirmatory
laboratory
techniques.
Clinically,
affected
pigs
exhibit
weight
loss,
jaundice,
poor
growth,
respiratory
distress,
gastrointestinal
issues.
Histopathological
examination
reveals
characteristic
lesions
such
as
lymphoid
depletion,
granulomatous
inflammation,
the
presence
inclusion
bodies
tissues.
Immunohistochemistry
(IHC)
situ
hybridization
(ISH)
are
critical
for
detecting
PCV2
antigens
viral
nucleic
acids
tissue
samples,
respectively.
Molecular
diagnostic
methods,
including
PCR
quantitative
(qPCR),
offer
rapid
precise
detection
DNA,
with
advancements
like
digital
droplet
(ddPCR)
improving
sensitivity.
Serological
tests,
immunofluorescence
assay
(IFA)
enzyme-linked
immunosorbent
(ELISA),
useful
antibodies,
aiding
assessing
infection
status
immune
response.
Genotyping
assays
help
track
evolution
strains,
essential
understanding
epidemiology
vaccine
development.
Prevention
control
involve
multifaceted
approach,
biosecurity
measures,
co-infection
management,
vaccination.
Vaccination
remains
key
tool,
updated
vaccines
providing
protection
against
multiple
genotypes.
Emerging
technologies,
nanobody-based
ELISAs
biosensors,
hold
promise
faster
more
cost-effective
diagnostics.
Continued
research
into
new
alongside
ongoing
surveillance
genetic
shifts,
effective
long-term
minimizing
its
impact
industry.
Язык: Английский