Descriptive Comparative Transcriptomic Analysis of Genotype IV SHEV ORF3-Expressing HepG2 Cells DOI Creative Commons
Hanwei Jiao,

Chi Meng,

Fengyuan Jiao

и другие.

Microorganisms, Год журнала: 2025, Номер 13(2), С. 412 - 412

Опубликована: Фев. 13, 2025

Background: Swine hepatitis E (HEV) is a zoonotic infectious disease caused by the swine virus (SHEV). Open reading frame 3 (ORF3) key virulence factor in HEV, playing crucial role release of viral particles, modulation host innate immune response, and regulation autophagy apoptosis, etc. However, its main function pathogenic mechanism remain incompletely understood. Results: In our study, adenoviruses ADV4-ORF3 ADV4-GFP were successfully constructed mediated overexpression enhanced green fluorescent protein (EGFP)-ORF3 EGFP HepG2 cells. A total 217 differentially expressed messenger RNAs (mRNAs) screened high-throughput sequencing, 27 statistically significant genes for further quantitative real-time reverse transcription (qRT-PCR) verification functional enrichment (Gene Ontology [GO] Kyoto Encyclopedia Genes Genomes [KEGG]). They are mainly involved six pathways: cellular response to unfolded protein, inflammatory cytokine activity, TNF signaling pathway, influenza A, pathways cancer. comparative analysis transcriptome mRNA expression profiles lncRNA results showed that mRNAs GPX1, MDM4, CLDN 39 transcripts overlapped have been identified. Conclusions: Eight differential genes, HSPA1A, HSPA1B, PLD3, RELA, GPI, SAMHD1, RPS6KA4, PIK3CB, verified. Comparing analyzing two sequencing methods indicated identified SHEV ORF3-expressing cells, which has laid genetic foundation physiological ORF3.

Язык: Английский

Descriptive Comparative Transcriptomic Analysis of Genotype IV SHEV ORF3-Expressing HepG2 Cells DOI Creative Commons
Hanwei Jiao,

Chi Meng,

Fengyuan Jiao

и другие.

Microorganisms, Год журнала: 2025, Номер 13(2), С. 412 - 412

Опубликована: Фев. 13, 2025

Background: Swine hepatitis E (HEV) is a zoonotic infectious disease caused by the swine virus (SHEV). Open reading frame 3 (ORF3) key virulence factor in HEV, playing crucial role release of viral particles, modulation host innate immune response, and regulation autophagy apoptosis, etc. However, its main function pathogenic mechanism remain incompletely understood. Results: In our study, adenoviruses ADV4-ORF3 ADV4-GFP were successfully constructed mediated overexpression enhanced green fluorescent protein (EGFP)-ORF3 EGFP HepG2 cells. A total 217 differentially expressed messenger RNAs (mRNAs) screened high-throughput sequencing, 27 statistically significant genes for further quantitative real-time reverse transcription (qRT-PCR) verification functional enrichment (Gene Ontology [GO] Kyoto Encyclopedia Genes Genomes [KEGG]). They are mainly involved six pathways: cellular response to unfolded protein, inflammatory cytokine activity, TNF signaling pathway, influenza A, pathways cancer. comparative analysis transcriptome mRNA expression profiles lncRNA results showed that mRNAs GPX1, MDM4, CLDN 39 transcripts overlapped have been identified. Conclusions: Eight differential genes, HSPA1A, HSPA1B, PLD3, RELA, GPI, SAMHD1, RPS6KA4, PIK3CB, verified. Comparing analyzing two sequencing methods indicated identified SHEV ORF3-expressing cells, which has laid genetic foundation physiological ORF3.

Язык: Английский

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