Etiological Spectrum of Acute Respiratory Infections in Bulgaria During the 2023–2024 Season and Genetic Diversity of Circulating Influenza Viruses DOI Creative Commons
Neli Korsun,

Ivelina Trifonova,

Diana T. Pavlova

и другие.

Viruses, Год журнала: 2025, Номер 17(2), С. 270 - 270

Опубликована: Фев. 16, 2025

Influenza poses a serious threat to both individual and public health. This study aimed investigate the virological epidemiological characteristics of influenza infections explore genetic diversity circulating viruses. In total, 1886 nasopharyngeal specimens from patients with acute respiratory illnesses were tested against 13 viruses using multiplex real-time PCR. Whole-genome sequencing, phylogenetic, amino acid analyses representative strains performed. At least one virus was detected in 869 (46.1%) patients; 87 (4.6%) co-infected two or three A(H1N1)pdm09 most prevalent (16.1%), followed by rhinoviruses (8.1%) RSV (6.7%). Hemagglutinin (HA) genes 74 categorized subclades C.1.8, C.1.9, C.1 within clade 5a.2a D1, D.2, D.3 5a.2a.1. The A(H3N2) analyzed belonged 2a.3a.1, J.2 J.1. sequenced B/Victoria lineage fell into V1A.3a.2, C.5.6 C.5.7. Amino substitutions viral proteins identified compared vaccine strains, including HA antigenic sites. demonstrated dominant distribution among studied

Язык: Английский

Diagnostic Significance of Influenza Symptoms and Signs, and Their Variation by Type/Subtype, in Outpatients Aged ≥ 15 Years: Novi Sad, Serbia DOI Creative Commons
Ana Miljković, Aleksandra Patić, Vladimir Petrović

и другие.

Viruses, Год журнала: 2025, Номер 17(2), С. 272 - 272

Опубликована: Фев. 16, 2025

This study assessed the diagnostic performance of influenza-related symptoms and signs their combinations, as well differences in patient characteristics based on type/subtype influenza, outpatients at a primary healthcare surveillance system. Our prospective analysis included cases aged ≥ 15 years from two influenza seasons (2022/23 2023/24) Novi Sad, Serbia. Influenza were confirmed using polymerase chain reaction (PCR) testing. The mean age participants with laboratory-confirmed was significantly lower than that those without (p < 0.0001): 37.90 vs. 54.92 2022/23, 40.21 54.17 2023/24. Among examined signs, highest sensitivity 2022/23 season demonstrated for fever (87.95%, CI: 78.96-94.07), while 2023/24 it cough (100.00%, 88.06-100.00). In season, positive predictive values (PPVs) (34.93%), chills (31.95%), myalgia (30.30%), malaise (28.57%), but they dropped all observed (ranging 1.91% to 9.17%). Compared World Health Organization's case definition influenza-like illness (ILI), provided by European Centre Disease Prevention Control higher specificity across both seasons. Participants who tested between December February more likely have A(H1N1)pdm09 or A(H3N2), whereas April B. underscores importance seasonal timing, symptom evaluation, definitions improving diagnosis care.

Язык: Английский

Процитировано

0

Etiological Spectrum of Acute Respiratory Infections in Bulgaria During the 2023–2024 Season and Genetic Diversity of Circulating Influenza Viruses DOI Creative Commons
Neli Korsun,

Ivelina Trifonova,

Diana T. Pavlova

и другие.

Viruses, Год журнала: 2025, Номер 17(2), С. 270 - 270

Опубликована: Фев. 16, 2025

Influenza poses a serious threat to both individual and public health. This study aimed investigate the virological epidemiological characteristics of influenza infections explore genetic diversity circulating viruses. In total, 1886 nasopharyngeal specimens from patients with acute respiratory illnesses were tested against 13 viruses using multiplex real-time PCR. Whole-genome sequencing, phylogenetic, amino acid analyses representative strains performed. At least one virus was detected in 869 (46.1%) patients; 87 (4.6%) co-infected two or three A(H1N1)pdm09 most prevalent (16.1%), followed by rhinoviruses (8.1%) RSV (6.7%). Hemagglutinin (HA) genes 74 categorized subclades C.1.8, C.1.9, C.1 within clade 5a.2a D1, D.2, D.3 5a.2a.1. The A(H3N2) analyzed belonged 2a.3a.1, J.2 J.1. sequenced B/Victoria lineage fell into V1A.3a.2, C.5.6 C.5.7. Amino substitutions viral proteins identified compared vaccine strains, including HA antigenic sites. demonstrated dominant distribution among studied

Язык: Английский

Процитировано

0