Synergistic Pathogenicity of Novel Duck Orthoreovirus and Salmonella Typhimurium in Ducks
Poultry Science,
Год журнала:
2025,
Номер
104(4), С. 104929 - 104929
Опубликована: Фев. 20, 2025
Co-infection
with
Novel
Duck
Orthoreovirus
(N-DRV)
and
Salmonella
Typhimurium
(S.
Typhimurium)
poses
significant
threats
to
duck
health.
The
disease
associated
N-DRV
is
characterized
by
epizootic
outbreaks
featuring
hepatosplenomegaly,
hemorrhage,
necrosis.
Ducklings
infected
STM
primarily
exhibit
symptoms
such
as
fibrinous
pericarditis,
perihepatitis,
intestinal
hemorrhage.
In
recent
years,
China
has
experienced
involving
mixed
infections
of
these
two
pathogens
in
various
regions,
leading
more
complex
atypical
clinical
manifestations.
This
study
investigated
the
synergistic
effects
through
animal
infection
models,
histopathological
analysis,
quantitative
PCR.
Antimicrobial
susceptibility
testing
confirmed
that
isolated
S.
strain
was
multidrug-resistant,
complicating
therapeutic
interventions.
Co-infected
ducklings
exhibited
higher
mortality
rates
(up
26.67
%),
earlier
peaks
viral
replication,
severe
symptoms,
including
diarrhea,
reluctance
move,
joint
swelling.
Viral
loads
immune
organs
spleen,
thymus,
bursa
Fabricius
were
elevated
co-infected
groups.
Histopathological
analysis
revealed
lesions
ducklings,
liver
necrosis,
splenic
hardening,
hemorrhages,
compared
single
infections.
Immunological
assessments
showed
suppressed
humoral
responses
groups,
indicating
N-DRV-induced
immunosuppression
facilitated
bacterial
colonization
systemic
dissemination.
These
findings
highlight
critical
importance
understanding
co-infection
dynamics
their
impact
on
host-pathogen
interactions.
provides
valuable
insights
for
developing
integrated
control
strategies
targeting
poultry,
promoting
improved
health
sustainable
production
farming
industry.
Язык: Английский
Phylogenetic Analysis and Pathogenicity of Avian Reoviruses Isolated from Viral Arthritis Cases in China 2010–2024
Veterinary Sciences,
Год журнала:
2025,
Номер
12(4), С. 307 - 307
Опубликована: Март 28, 2025
Avian
reovirus
(ARV)
is
one
of
the
main
causes
viral
arthritis,
tenosynovitis,
malabsorption
syndrome
(MAS),
runting-stunting
syndrome,
and
immunodepression.
In
recent
years,
due
to
emergence
new
ARV
strains,
outbreaks
disease
have
brought
significant
economic
losses
chicken
flocks.
To
determine
prevalence
in
China
from
2010
2024,
a
total
409
tissue
samples
different
breeding
farms
were
collected
chickens
presenting
clinical
signs
lameness
swollen
joints
various
flocks
located
18
provinces.
As
performed
on
these
samples,
ARV-specific
reverse
transcription-polymerase
chain
reaction
(RT-PCR)
assay
indicated
111
ARV-positive
with
positive
rate
27.14%.
After
isolation
necropsied
69
strains
isolated,
specific
sigma
C
(σC)
genes
amplified
sequenced.
The
sequence
analysis
σC
showed
that
isolates
grouped
into
six
clusters,
including
14
cluster
I
(20.29%),
12
II
(17.39%),
3
III
(4.35%),
8
IV
(11.59%),
V
29
VI
(42.03%).
Except
for
V,
each
other
five
clusters
could
be
divided
two
subclusters.
Homology
II–VI
had
only
50.3
60.8%
homology
commercial
S1133
vaccine
strain
which
derived
I.
ARVs
subcluster
Ia
high
(93.5–98.0%),
while
Ib
low
(73.4–76.4%).
Further,
viruses,
epidemic
genotype
China,
50.3–55.7%
strain.
results
pathogenicity
test
representative
all
caused
swelling
footpads
SPF
chickens,
incidence
was
not
significantly
different.
present
study
will
helpful
understanding
revealed
genetic
differences
between
Язык: Английский
Isolation, Identification, and Pathogenicity of an Avian Reovirus Epidemic Strain in Xinjiang, China
Viruses,
Год журнала:
2025,
Номер
17(4), С. 499 - 499
Опубликована: Март 30, 2025
To
investigate
the
prevalence
and
pathogenic
characteristics
of
avian
reovirus
(ARV)
in
Xinjiang
region
China,
this
study
collected
suspected
joint
tissue
samples
from
broiler
farms
across
different
areas
Taikun
Group.
The
were
subjected
to
virus
isolation,
RT-PCR
analysis,
sequence
vitro
replication
assays,
pathogenicity
assessments
specific
pathogen-free
(SPF)
chicken
embryos
chickens.
results
revealed
isolation
a
chicken-derived
ARV
epidemic
strain,
designated
as
xj-1.1.
virus-induced
cytopathic
effects
(CPEs)
LMH
cells
time
required
observe
CPEs
significantly
decreased
with
serial
blind
passages.
Pathogenicity
testing
demonstrated
that
xj-1.1
is
highly
virulent
SPF
Inoculation
resulted
100%
mortality
rate,
while
inoculation
1-day-old
chicks
caused
significant
swelling
footpads.
In
conclusion,
successfully
isolated
an
strain
reovirus,
providing
valuable
theoretical
basis
for
understanding
genetic
evolution
epidemiology
variants
region.
Язык: Английский