Insight into the Role of the Aryl Hydrocarbon Receptor in Bovine Coronavirus Infection by an Integrated Approach Combining In Vitro and In Silico Methods DOI Creative Commons

Luca Del Sorbo,

Clementina Acconcia, Maria Michela Salvatore

и другие.

Microorganisms, Год журнала: 2025, Номер 13(3), С. 579 - 579

Опубликована: Март 4, 2025

It is well known that the host response to different human and animal coronaviruses infection regulated by aryl hydrocarbon receptor, a ligand-activated transcription factor. The present study investigates expression of receptor during bovine coronavirus infection, through in vitro silico investigations. studies demonstrate as its targets, CYP1A1 CYP1B1, were significantly activated cells (MDBK). During pretreatment with non-cytotoxic doses CH223191, selective inhibitor resulted significant reduction virus yield downregulation viral spike protein expression. These findings occurred presence inhibition signaling. Our results reveal acts on replication, upregulating downstream target proteins, CYP1B1. In addition, following studies, three-dimensional structural model complex antagonist CH223191 indicates molecular mechanism, which PASB TAD domains interact inhibitor, mainly driven an extensive network hydrophobic interactions, series hydrogen bonds contributing stabilizing complex. Interestingly, bioinformatic analyses revealed high similarity at primary sequence structure levels. Taken together, these represent fundamental step for development innovative drugs targeting AhR potential object CoVs therapy.

Язык: Английский

Insight into the Role of the Aryl Hydrocarbon Receptor in Bovine Coronavirus Infection by an Integrated Approach Combining In Vitro and In Silico Methods DOI Creative Commons

Luca Del Sorbo,

Clementina Acconcia, Maria Michela Salvatore

и другие.

Microorganisms, Год журнала: 2025, Номер 13(3), С. 579 - 579

Опубликована: Март 4, 2025

It is well known that the host response to different human and animal coronaviruses infection regulated by aryl hydrocarbon receptor, a ligand-activated transcription factor. The present study investigates expression of receptor during bovine coronavirus infection, through in vitro silico investigations. studies demonstrate as its targets, CYP1A1 CYP1B1, were significantly activated cells (MDBK). During pretreatment with non-cytotoxic doses CH223191, selective inhibitor resulted significant reduction virus yield downregulation viral spike protein expression. These findings occurred presence inhibition signaling. Our results reveal acts on replication, upregulating downstream target proteins, CYP1B1. In addition, following studies, three-dimensional structural model complex antagonist CH223191 indicates molecular mechanism, which PASB TAD domains interact inhibitor, mainly driven an extensive network hydrophobic interactions, series hydrogen bonds contributing stabilizing complex. Interestingly, bioinformatic analyses revealed high similarity at primary sequence structure levels. Taken together, these represent fundamental step for development innovative drugs targeting AhR potential object CoVs therapy.

Язык: Английский

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