Importance of Viral Late Domains in Budding and Release of Enveloped RNA Viruses DOI Creative Commons

Lisa Welker,

Jean‐Christophe Paillart, Serena Bernacchi

и другие.

Viruses, Год журнала: 2021, Номер 13(8), С. 1559 - 1559

Опубликована: Авг. 6, 2021

Late assembly (L) domains are conserved sequences that necessary for the late steps of viral replication, acting like cellular adaptors to engage ESCRT membrane fission machinery promote virion release. These short sequences, whose mutation or deletion produce accumulation immature virions at plasma membrane, were firstly identified within retroviral Gag precursors, and in a further step, also structural proteins many other enveloped RNA viruses including arenaviruses, filoviruses, rhabdoviruses, reoviruses, paramyxoviruses. Three classes L have been thus far (PT/SAP, YPXnL/LXXLF, PPxY), even if it has recently suggested motifs could act as domains. Here, we summarize current state knowledge different types their partners budding events viruses, with particular focus on retroviruses.

Язык: Английский

Arc – An endogenous neuronal retrovirus? DOI Creative Commons
Jason D. Shepherd

Seminars in Cell and Developmental Biology, Год журнала: 2017, Номер 77, С. 73 - 78

Опубликована: Сен. 24, 2017

Язык: Английский

Процитировано

48
Retroviral Gag protein–RNA interactions: Implications for specific genomic RNA packaging and virion assembly DOI
Erik D. Olson,

Karin Musier‐Forsyth

Seminars in Cell and Developmental Biology, Год журнала: 2018, Номер 86, С. 129 - 139

Опубликована: Апрель 1, 2018

Язык: Английский

Процитировано

46
The evolution of RNA structural probing methods: From gels to next‐generation sequencing DOI
Élodie Mailler, Jean‐Christophe Paillart, Roland Marquet

и другие.

Wiley Interdisciplinary Reviews - RNA, Год журнала: 2018, Номер 10(2)

Опубликована: Ноя. 28, 2018

RNA molecules are important players in all domains of life and the study relationship between their multiple flexible states associated biological roles has increased recent years. For several decades, chemical enzymatic structural probing experiments have been used to determine structure. During this time, there a steady improvement reagents experimental methods, today biologist community large range tools at its disposal probe secondary structure RNAs vitro cells. Early radioactive labeling polyacrylamide gel electrophoresis as read‐out methods. This was superseded by capillary electrophoresis, more recently next‐generation sequencing. Today, powerful methods can characterize on genome‐wide scale. In review, we will provide an overview methodologies from historical technical perspective. article is categorized under: Structure Dynamics > Structure, Dynamics, Chemistry Methods Analyses Silico Cells

Язык: Английский

Процитировано

41
Transposon control mechanisms in telomere biology DOI

Maria Kordyukova,

Ivan Olovnikov, Alla Kalmykova

и другие.

Current Opinion in Genetics & Development, Год журнала: 2018, Номер 49, С. 56 - 62

Опубликована: Март 20, 2018

Язык: Английский

Процитировано

40
The C-terminal p6 domain of the HIV-1 Pr55Gagprecursor is required for specific binding to the genomic RNA DOI Open Access

Noé Dubois,

Keith K. Khoo,

Shannon Ghossein

и другие.

RNA Biology, Год журнала: 2018, Номер 15(7), С. 923 - 936

Опубликована: Июнь 29, 2018

The Pr55Gag precursor specifically selects the HIV-1 genomic RNA (gRNA) from a large excess of cellular and partially or fully spliced viral RNAs drives virus assembly at plasma membrane. During these processes, NC domain interacts with gRNA, while its C-terminal p6 binds factors orchestrates particle release. Gag∆p6 is truncated form lacking usually used as default surrogate for wild type in vitro analysis. With recent advance production full-length recombinant Pr55Gag, here, we tested whether also contributes to binding specificity by systematically comparing panel RNAs. Unexpectedly, our fluorescence data reveal that absolutely required specific gRNA. Its deletion resulted not only decreased affinity but an increased In contrast displayed similar all Removal His-tag uniformly Kd values RNA-protein complexes ~ 2.5 fold did affect specificities proteins. Altogether, results demonstrate novel role Pr55Gag-RNA interactions, strongly suggest discrimination gRNA mRNAs, which necessary selective encapsidation.

Язык: Английский

Процитировано

40
Arc self‐association and formation of virus‐like capsids are mediated by an N‐terminal helical coil motif DOI Creative Commons
Maria Steene Eriksen, Oleksii Nikolaienko, Erik I. Hallin

и другие.

FEBS Journal, Год журнала: 2020, Номер 288(9), С. 2930 - 2955

Опубликована: Ноя. 11, 2020

Activity‐regulated cytoskeleton‐associated protein (Arc) is a interaction hub with diverse roles in intracellular neuronal signaling, and important functions synaptic plasticity, memory, postnatal cortical development. Arc has homology to retroviral Gag capable of self‐assembly into virus‐like capsids implicated the intercellular transfer RNA. However, molecular basis self‐association capsid formation largely unknown. Here, we identified 28‐amino‐acid stretch mammalian N‐terminal (NT) domain that necessary sufficient for self‐association. Within this region, 7‐residue oligomerization motif, critical capsids. Purified wild‐type formed as shown by transmission cryo‐electron microscopy, whereas mutant disruption motif homogenous dimers. An atomic‐resolution crystal structure region peptide demonstrated an antiparallel coiled‐coil interface, strongly supporting NT‐NT interactions oligomerization. The NT coil–coil was also validated live neurons using fluorescence lifetime FRET imaging, mutation disrupted Arc‐facilitated endocytosis. Furthermore, single‐molecule photobleaching, show mRNA greatly enhances higher‐order manner dependent on motif. In conclusion, helical coil supports above dimer stage, mRNA‐induced oligomerization, Database coordinates factors crystallographic analysis were deposited at Protein Data Bank entry code 6YTU .

Язык: Английский

Процитировано

37
Molecular physiology of Arc/Arg3.1: The oligomeric state hypothesis of synaptic plasticity DOI
Maria Steene Eriksen, Clive R. Bramham

Acta Physiologica, Год журнала: 2022, Номер 236(3)

Опубликована: Сен. 8, 2022

Abstract The immediate early gene, Arc, is a pivotal regulator of synaptic plasticity, memory, and cognitive flexibility. But what Arc protein? How does it work? Inside the neuron, protein interaction hub dynamic intra‐cellular signaling in plasticity. In remarkable contrast, can also self‐assemble into retrovirus‐like capsids that are released extracellular vesicles capable intercellular transfer RNA. Elucidation molecular basis capsid functions, relationship between them, vital for progress. Here, we discuss recent findings on structure–function regulation oligomerization giving insight physiology Arc. unique features mammalian emphasized, while drawing comparisons with Drosophila retroviral Gag. N‐terminal domain, found only mammals, proposed to play key role regulating signaling, oligomerization, formation capsids. Bringing together several lines evidence, hypothesize function plasticity—long‐term potentiation (LTP) long‐term depression (LTD)—are dictated by different oligomeric forms Specifically, monomer/dimer LTP, tetramer basic LTD, 32‐unit oligomer enhanced LTD. unclear but likely depends cross‐section captured neuronal activity‐induced RNAs. As functional states revealed, may be possible selectively manipulate specific Arc‐dependent plasticity communication involved brain dysfunction.

Язык: Английский

Процитировано

21
NMR Studies of the Structure and Function of the HIV-1 5′-Leader DOI Creative Commons
Sarah C. Keane,

Michael Summers

Viruses, Год журнала: 2016, Номер 8(12), С. 338 - 338

Опубликована: Дек. 21, 2016

The 5′-leader of the human immunodeficiency virus type 1 (HIV-1) genome plays several critical roles during viral replication, including differentially establishing mRNA versus genomic RNA (gRNA) fates. As observed for proteins, function is tightly regulated by its structure, and a common paradigm has been that temporally modulated structural changes in 5′-leader. Over past 30 years, combinations nucleotide reactivity mapping experiments with biochemistry, mutagenesis, phylogenetic studies have provided clues regarding secondary structures stretches residues within leader adopt functionally discrete domains. More recently, nuclear magnetic resonance (NMR) spectroscopy approaches developed enable direct detection intra- inter-molecular interactions intact leader, providing detailed insights into determinants mechanisms regulate HIV-1 packaging function.

Язык: Английский

Процитировано

38
Know Your Enemy: Successful Bioinformatic Approaches to Predict Functional RNA Structures in Viral RNAs DOI Creative Commons
Chun Shen Lim, Chris M. Brown

Frontiers in Microbiology, Год журнала: 2018, Номер 8

Опубликована: Янв. 4, 2018

Structured RNA elements may control virus replication, transcription and translation, their distinct features are being exploited by novel antiviral strategies. Viral continue to be discovered using combinations of experimental computational analyses. However, the wealth sequence data, notably from deep viral sequencing, viromes, metagenomes, necessitates approaches used as an essential discovery tool. In this review, we describe practical discover functional in genomes. addition success stories new emerging viruses, these have revealed some surprising well-studied viruses e.g., human immunodeficiency virus, hepatitis C influenza, dengue viruses. Some notable discoveries were facilitated comparative analyses diverse genome alignments. Importantly, for finding embedded coding non-coding regions differ. With exponential growth computer power progressed stem-loop prediction on single sequences cutting edge 3D prediction, command line user friendly web interfaces. Despite advances, many powerful, tools resources underutilized virology community.

Язык: Английский

Процитировано

35
Oleanolic Acid Derivatives as Potential Inhibitors of HIV-1 Protease DOI
Marta Medina‐O’Donnell, Francisco Rivas, Fernando J. Reyes‐Zurita

и другие.

Journal of Natural Products, Год журнала: 2019, Номер 82(10), С. 2886 - 2896

Опубликована: Окт. 16, 2019

Pentacyclic triterpenes, such as oleanolic acid (I), are promising scaffolds for diversification through the use of combinatorial methods to obtain derivatives that improve their biological properties, increasing bioavailability and enhancing therapeutic efficacy. The purpose this study was evaluate influence acid, conjugated with one or two amino acids an acyl group, might exert on HIV-1 protease inhibition. in vitro studies conducted suggested presence a carboxyacyl group generally improves inhibition protease, especially when phthaloyl is present, IC50 concentration values below 5 μM. gain activity three 3-phthaloyl derivatives, sub-micromolar values, between 60- 100-fold more active than acid. A molecular docking has also been performed elucidate mode binding by these derivatives. In general, exhibited highest inhibitory showed energies simulations. overall results suggest coupling inhibition, implying triterpene may be antiviral agents against HIV.

Язык: Английский

Процитировано

35