Monitoring hand foot and mouth disease using long-term wastewater surveillance in Japan: Quantitative PCR assay development and application

The Science of The Total Environment, Год журнала: 2023, Номер 901, С. 165926 - 165926

Опубликована: Июль 30, 2023

Hand, foot, and mouth disease (HFMD) is a highly contagious that primarily affects children under five years of age. It mainly caused by serotypes Enterovirus A (EVA): EVA71, Coxsackievirus types 6 (CVA6), 10 (CVA10), 16 (CVA16). Despite being prevalent in Japan other countries the Asia-Pacific region, few studies have investigated HFMD pathogens wastewater. The present study aimed to develop sensitive broadly reactive quantitative polymerase chain reaction (qPCR) assay dominant serotype CVA6, revise previously developed CVA10, CVA16 assays, test these assays wastewater samples from Yamanashi Prefecture, Japan. new-CVA6 qPCR was with maximal nucleotide percent identity among CVA6 isolates revised were had ability quantify respective positive controls at levels as low 1 copy/μL. Among 53 grab influent collected between March 2022 2023, RNA not detected any samples, whereas could detect 38 % (20/53) samples. significantly higher concentration summer season (3.3 ± 0.8 log10 copies/L; 79 (11/14)) than autumn (2.7 0.6 69 (9/13)). seasonal trend detection aligned case reports catchment treatment plant. This first report trends EVA associated provides evidence wastewater-based epidemiology applicable even for diseases are only specific population groups.

Язык: Английский

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Development of Bayesian segmented Poisson regression model to forecast COVID-19 dynamics based on wastewater data: a case study in Nanning City, China

BMC Public Health, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 9, 2025

Язык: Английский

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Targeted Enrichment Sequencing Utilizing a Respiratory Pathogen Panel for Genomic Wastewater-Based Viral Epidemiology in Uruguay

Food and Environmental Virology, Год журнала: 2025, Номер 17(1)

Опубликована: Янв. 9, 2025

Язык: Английский

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A Perspective on Wastewater and Environmental Surveillance as a Public Health Tool for Low- and Middle-Income Countries

Microorganisms, Год журнала: 2025, Номер 13(2), С. 238 - 238

Опубликована: Янв. 22, 2025

Geographical variations in infectious diseases create differences public health priorities between high- and low-income countries. Low- middle-income countries (LMICs) face resource constraints that limit adherence to international monitoring standards for wastewater-based epidemiology (WBE). The development of low-cost WBE programs, such as those detect SARS-CoV-2, offers LMICs a promising tool pathogens local concern. In this work, we summarize important wastewater biomarkers their associated challenges, ranging from causing gastroenteritis putative markers plant linked food safety, well antimicrobial resistance. We raise awareness the great potential highlight critical markers, research needs, strategies necessary establish tailored surveillance programs.

Язык: Английский

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Wastewater-based epidemiology of SARS-CoV-2 and the development of early warning system: challenges and lessons learned after two years of weekly surveillance in the Brazilian Federal District

Опубликована: Фев. 1, 2025

Язык: Английский

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