Characterisation of High Alkaline-Tolerant Novel Ulvan Lyase from Pseudoalteromonas agarivorans: Potential Applications of Enzyme Derived Oligo-Ulvan as Anti-Diabetic Agent DOI Creative Commons

Navindu Dinara Gajanayaka,

Eunyoung Jo,

Minthari Sakethanika Bandara

и другие.

Marine Drugs, Год журнала: 2024, Номер 22(12), С. 577 - 577

Опубликована: Дек. 23, 2024

Green algae, particularly Ulva species, are rich in complex polysaccharides, such as ulvan, which have significant potential for biotechnological applications. However, the biochemical properties of ulvan depolymerised products remain underexplored. The enzymatic depolymerisation has garnered attention owing to its cost advantages over alternative methods. Nevertheless, characterisation lyases, specifically those belonging polysaccharide lyase family 25 (PL25), is limited. In this study, we identified and biochemically characterised a novel PL25 lyase, PaUL25, functions optimally at pH 10. Additionally, explored alpha (α)-glucosidase inhibitory products. PaUL25 exhibited optimum activity 35 °C Tris-HCl buffer (pH 10). Moreover, enzyme was enhanced by more than 150% presence Mn2+ metal ions below concentrations 10 mM. endolytic action produced oligosaccharides with degrees polymerisation 2 4 end Partially completely hydrolysed α-glucosidase activity, half concentration IC50 values 3.21 ± 0.13 2.51 0.19 mg/mL, respectively. These findings expand our understanding highlight pharmaceutical oligosaccharides, antidiabetic agents.

Язык: Английский

Microalgae: a multifaceted catalyst for sustainable solutions in renewable energy, food security, and environmental management DOI Creative Commons
Byung Sun Yu,

Seonju Pyo,

J. Lee

и другие.

Microbial Cell Factories, Год журнала: 2024, Номер 23(1)

Опубликована: Ноя. 14, 2024

This review comprehensively examines the various applications of microalgae, focusing on their significant potential in producing biodiesel and hydrogen, serving as sustainable food sources, efficacy treating both municipal food-related wastewater. While previous studies have mainly focused specific such biofuel production or wastewater treatment, this covers these comprehensively. It for microalgae to be applied industrial sectors energy, security, environmental management. By bridging different application areas, differs from providing an integrated multifaceted view microalgae. Since it is essential increase productivity process utilize applications, research trends cultivation processes, including culture system (e.g., open ponds, closed ponds) conditions pH, temperature, light intensity) improve biomass valuable substances was firstly analyzed. In addition, technologies that can maximize while limiting contamination occur when utilizing systems been described CO2 reduction. conclusion, has provided a detailed analysis current findings technological innovations, highlighting important role addressing global challenges related supply, waste also insights into future directions commercial several bio-related industries, illustrated how continued exploration development area realize full

Язык: Английский

Процитировано

5

Science and Technology for Water Purification: Achievements and Strategies DOI Open Access
Yuanfeng Qi, Kai He

Water, Год журнала: 2025, Номер 17(1), С. 91 - 91

Опубликована: Янв. 1, 2025

Water scarcity and pollution remain two of the most pressing global challenges, with profound implications for public health, environmental sustainability, economic development [...]

Язык: Английский

Процитировано

0

Persistent and Emerging Organic Contaminants in Natural Environments DOI Open Access
Jasmin Rauseo, Francesca Spataro, Luisa Patrolecco

и другие.

Water, Год журнала: 2025, Номер 17(3), С. 436 - 436

Опубликована: Фев. 5, 2025

In recent decades, the world has experienced detrimental effects of unchecked growth various human activities, including industrialization, transportation, agriculture, and urbanization [...]

Язык: Английский

Процитировано

0

Bioremediation of Synthetic Wastewater with Contaminants of Emerging Concern by Nannochloropsis sp. and Lipid Production: A Circular Approach DOI Creative Commons
B. C. dos Santos, Juliana Araújo, BEATRIZ MACIEL DE CARVALHO

и другие.

Bioengineering, Год журнала: 2025, Номер 12(3), С. 246 - 246

Опубликована: Фев. 28, 2025

Contaminants of emerging concern (CECs) pose a potential risk to human and environmental health. Microalgae bioremediation is promising approach for transforming or removing contaminants from the environment, while contributing circular economy. In this study, Nannochloropsis sp. was effectively used simultaneous removal six CECs: paracetamol, ibuprofen, imidacloprid, methylparaben bisphenol A at 10 µg mL−1 triclosan 0.5 synthetic wastewater, which were able survive under such concentrations, higher than those commonly found in environment (up 2.82 methylparaben). High efficiencies reached (100%) (93 ± 2%), paracetamol 30 1%, 64 2% 49 5% removed, respectively. Subsequently, lipids extracted, FAME profile characterised using GS-MS. The main fatty acids identified after hexadecadienoic acid isomers (C16:2), palmitic (C16), linoleic (C18:2) γ-linolenic (C18:3). absence oleic stearic noticed, suggesting an alteration lipidic due contaminant exposure. By exploring quantification future work, applications extracted can be explored, further demonstrating feasibility process.

Язык: Английский

Процитировано

0

Optimization and Kinetic Studies of Aquaculture Effluent Bioremediation Using Chlorococcum sp. DOI Creative Commons
Zubair Hashmi, Ibrahim Maina Idriss, Rieza Zulrian Aldio

и другие.

Cleaner Waste Systems, Год журнала: 2025, Номер unknown, С. 100304 - 100304

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Microalgae-Assisted Treatment of Wastewater Originating from Varied Sources, Particularly in the Context of Heavy Metals and Antibiotic-Resistant Bacteria DOI Open Access
Rabia Rehman, Syeda Fazoon Kazmi, Muhammad Irshad

и другие.

Water, Год журнала: 2024, Номер 16(22), С. 3305 - 3305

Опубликована: Ноя. 18, 2024

The increasing prevalence of heavy metals and antibiotic-resistant bacteria in wastewater (WW) raises serious environmental public health concerns. This study investigates the efficiency microalgal strain Chlorella vulgaris EV-465 treating evaluates antibiotic resistance profile bacterial strains obtained from WW samples. was used to treat four types wastewater—domestic, municipal, hospital, industrial wastewater—through 21 days incubation. findings demonstrated pH stabilization significant decreases nutrients (total nitrogen—TN, total phosphorus—TP), biological oxygen demand (BOD), chemical (COD), metal (HM) concentrations, count. Copper (Cu) showed highest reduction, decreasing by 80% within 14 days, while lead (Pb) proved more resistant removal, with only a 50% decrease day 21. Additionally, algae decreased counts, lowering colony-forming units (Log CFU/mL) 9.04 4.65 municipal over 21-day period. Antibiotic susceptibility tests for isolated revealed high levels resistance, seven out nine exhibiting multidrug resistance. Notably, Enterococcus faecium (PBI08), Acinetobacter baumannii (YBH19), Pseudomonas aeruginosa (NBH16) displayed all antibiotics tested. Among tested antibiotics, Ciprofloxacin efficacy, 66% strains. Cluster phylogenetic analyses that majority belonged genera Escherichia, highlighting their genetic diversity varied mechanisms.

Язык: Английский

Процитировано

1

Innovative strategies for utilizing microalgae as dual-purpose biofertilizers and phycoremediators in agroecosystems DOI Creative Commons
Muhamad Maulana Azimatun Nur,

Mahreni,

Sri Wahyu Murni

и другие.

Biotechnology Reports, Год журнала: 2024, Номер 45, С. e00870 - e00870

Опубликована: Дек. 10, 2024

Язык: Английский

Процитировано

1

Characterisation of High Alkaline-Tolerant Novel Ulvan Lyase from Pseudoalteromonas agarivorans: Potential Applications of Enzyme Derived Oligo-Ulvan as Anti-Diabetic Agent DOI Creative Commons

Navindu Dinara Gajanayaka,

Eunyoung Jo,

Minthari Sakethanika Bandara

и другие.

Marine Drugs, Год журнала: 2024, Номер 22(12), С. 577 - 577

Опубликована: Дек. 23, 2024

Green algae, particularly Ulva species, are rich in complex polysaccharides, such as ulvan, which have significant potential for biotechnological applications. However, the biochemical properties of ulvan depolymerised products remain underexplored. The enzymatic depolymerisation has garnered attention owing to its cost advantages over alternative methods. Nevertheless, characterisation lyases, specifically those belonging polysaccharide lyase family 25 (PL25), is limited. In this study, we identified and biochemically characterised a novel PL25 lyase, PaUL25, functions optimally at pH 10. Additionally, explored alpha (α)-glucosidase inhibitory products. PaUL25 exhibited optimum activity 35 °C Tris-HCl buffer (pH 10). Moreover, enzyme was enhanced by more than 150% presence Mn2+ metal ions below concentrations 10 mM. endolytic action produced oligosaccharides with degrees polymerisation 2 4 end Partially completely hydrolysed α-glucosidase activity, half concentration IC50 values 3.21 ± 0.13 2.51 0.19 mg/mL, respectively. These findings expand our understanding highlight pharmaceutical oligosaccharides, antidiabetic agents.

Язык: Английский

Процитировано

0