Stabilized Extracellular Vesicle Formulations for Inhalable Dry Powder Development
Small,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 13, 2025
Abstract
Chronic
obstructive
pulmonary
disease
(COPD)
is
the
third
leading
cause
of
death
worldwide,
with
a
significant
impact
on
low‐
and
middle‐income
countries,
making
it
critical
challenge
for
global
health
equity
sustainable
development
goals.
Extracellular
vesicles
(EVs)
are
emerging
as
promising
treatment
COPD,
but
conventional
storage
at
−80
°C
limits
their
accessibility.
This
study
explores
alternative
methods
to
enhance
EV
stability
accessibility,
particularly
in
low‐resource
settings.
EVs
from
lung
fibroblasts
subjected
freezing,
freeze
drying,
spray
drying
inulin
or
mannitol.
The
biophysical
properties
evaluated
by
capacity
support
organoid
formation.
Inulin
proves
effective
stabilizing
EVs,
maintaining
functionality
after
freezing
−20
12
weeks.
Freeze
preserves
20
43%
relative
humidity.
In
contrast,
mannitol
exhibit
aggregation
reduce
bio‐functionality.
Additionally,
leucine
produces
dry
powder
suitable
inhalation,
demonstrates
efficient
deposition
using
Cyclops
inhaler.
These
findings
suggest
stabilizer
eliminating
need
ultra‐low
temperature
improving
practicality
EV‐based
inhalable
therapies.
Язык: Английский
Mitochondria transfer for myelin repair
Journal of Cerebral Blood Flow & Metabolism,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 13, 2025
Demyelination
is
a
common
feature
of
neuroinflammatory
and
degenerative
diseases
the
central
nervous
system
(CNS),
such
as
multiple
sclerosis
(MS).
It
often
linked
to
disruptions
in
intercellular
communication,
bioenergetics
metabolic
balance
accompanied
by
mitochondrial
dysfunction
cells
oligodendrocytes,
neurons,
astrocytes,
microglia.
Although
current
MS
treatments
focus
on
immunomodulation,
they
fail
stop
or
reverse
demyelination’s
progression.
Recent
advancements
highlight
exchange
promising
therapeutic
target,
with
potential
restore
homeostasis,
enhance
promote
myelin
repair.
With
this
review
we
will
provide
insights
into
CNS
decoupling,
focusing
role
demyelinating
conditions.
We
then
discuss
emerging
cell-free
biotherapies
exploring
transferring
mitochondria
via
biogenic
carriers
like
extracellular
vesicles
(EVs)
synthetic
liposomes,
aimed
at
enhancing
function
support
for
Lastly,
address
key
challenges
clinical
application
these
strategies
future
directions
optimize
biotherapies.
The
field
hold
promise
restoring
repair,
potentially
transforming
landscape
diseases.
Язык: Английский
MSC-derived extracellular vesicles: Precision miRNA delivery for overcoming cancer therapy resistance
Regenerative Therapy,
Год журнала:
2025,
Номер
29, С. 303 - 318
Опубликована: Апрель 5, 2025
Язык: Английский
Functionality of Lyophilized Engineered EVs: A Mechanistic Study
Опубликована: Янв. 1, 2024
Mesenchymal
stem
cell
derived
extracellular
vesicles
(MSC
EVs)
possess
immense
therapeutic
potential.
Over
the
past
several
years
many
groups
have
identified
unique
ways
to
engineer
these
EVs
for
pathway
specific
activity.
Our
laboratory
has
characterized
EV
bioactivity
mechanistically
as
well
devised
approaches
regenerative
medicine
applications.
For
advances
be
clinically
relevant,
translational
therapeutics
need
evaluated
and
established.
Long-term
stability
storage
of
purified
is
one
major
hurdles
facing
translation
therapeutics.
Lyophilization/freeze
drying
way
preserve
at
room
temperature.
To
achieve
this
without
damage
membranes,
multiple
studies
various
cryoprotectants
freezing
lyophilization.
While
focused
extensively
on
biophysical
characterization
lyophilized
EVs,
functional,
mechanistic
in
vivo
functionality
remained
severely
understudied.
Using
functionally
engineered
osteoinductive
developed
our
laboratory,
study,
we
endeavored
compare
functional
fresh
vitro
using
dimethyl
sulfoxide
(DMSO)
a
cryoprotectant.
Results
presented
here
highlight
ability
DMSO
maintain
serve
baseline
its
potential
Future
can
focus
expanding
knowledge
test
other
naïve
regeneration
tissues.
Язык: Английский
Functionality of lyophilized osteoinductive EVs: a mechanistic study
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
12
Опубликована: Окт. 22, 2024
Introduction
Mesenchymal
stem
cell-derived
extracellular
vesicles
(MSC
EVs)
hold
significant
promise
for
regenerative
medicine.
Lyophilization
of
EVs
significantly
enhances
their
translational
potential.
While,
lyophilized
have
been
studied
from
a
morphological
perspective,
the
functional
stability
these
and
cargo
following
lyophilization
need
to
be
mechanistically
investigated.
Methods
In
this
study,
we
investigated
mechanistic
bioactivity
fresh
versus
MSC
EVs,
specifically
focusing
on
functionally
engineered
osteoinductive
developed
in
our
laboratory.
We
utilized
dimethyl
sulfoxide
(DMSO)
as
cryoprotectant
conducted
pathway-specific
vitro
vivo
experiments
assess
functionality
EVs.
Results
Our
findings
show
that
using
DMSO
before
preserves
demonstrated
endocytosis,
integrity,
activity
were
maintained
when
was
used
cryoprotectant.
Additionally,
bone
regeneration
studies
revealed
cryoprotected
comparable
freshly
isolated
Discussion
These
results
provide
foundation
evaluating
exploring
use
other
cryoprotectants
EV-based
therapies.
Understanding
naïve
perspective
may
enhance
validation
approaches
tissue
strategies.
Язык: Английский