Arched Microfluidic Channel for the Promotion of Axonal Growth Performance DOI Creative Commons
Menghua Liu, Anping Wu, Jiaxin Liu

и другие.

iScience, Год журнала: 2024, Номер 27(10), С. 110885 - 110885

Опубликована: Сен. 4, 2024

Uniformly distributed fluid shear stress can promote axonal growth, aiding in the efficient construction of functional neural interfaces. However, challenges remain micro-scale environment with a uniform fluidic distribution. In this study, we designed and fabricated microfluidic chip arched-section channels (AMCs) to increase primary cortical neuron growth rate terminal number by constructing uniform-stress-distributed environment. Inspired three-dimensional (3D) microenvironment where cerebrospinal-fluid-contacting neurons are located, surface curvature traditional rectangular-section channel (RMC) was adjusted construct structures 3D curved surfaces. Compared those on RMC chips, average axons AMC chips increased 8.9% within 19 days, terminals 14.9%. This platform provides structure that effectively has potential more complex

Язык: Английский

Review of the Applications of Micro/Nanorobots in Biomedicine DOI
He Zhang, Jun Tang, Huiliang Cao

и другие.

ACS Applied Nano Materials, Год журнала: 2024, Номер 7(15), С. 17151 - 17192

Опубликована: Июль 24, 2024

The advancement of precision medicine in modern biomedicine has been significantly propelled by the emergence micro/nanorobots. combination micro/nanorobots and offers unique advantages holds attractive potential for applications. It effectively addresses challenges encountered traditional robots areas such as driving, sensing, intelligence other aspects, especially application cancer cardiovascular diseases, which is a hot field interdisciplinary research recent years. This review succinctly delineates analyzes advancements First, achievements years are systematically classified introduced from four perspectives: manufacturing design, drive control, biomedical degradation. In particular, in-depth discussions were conducted regarding solutions each stage. Second, based on latest results applications, development direction industrial applications key technical aspects that need to be paid attention emphasized. Finally, we discuss opportunities future, it expected will become more sophisticated capable performing multiple medical functions tasks. They implemented vivo assist doctors diagnosing treating diseases. shows significant medicine.

Язык: Английский

Процитировано

12

A Vascularized Multilayer Chip Reveals Shear Stress Induced Angiogenesis in Diverse Fluid Conditions DOI Creative Commons
Tao Yue, Hui Ying Yang,

Yue Wang

и другие.

Cyborg and Bionic Systems, Год журнала: 2025, Номер 6

Опубликована: Янв. 1, 2025

Tissues larger than 400 μm in size lacking microvascular networks cannot survive for long periods of time vitro. The development microfluidic technology provides an efficient research tool constructing models However, traditional single-layer chips faced the limitation spatial layout and could not provide diverse fluidic environments within a single chip. In this paper, we present novel chip design with 3-layer configuration that utilizes polycarbonate (PC) porous membrane to separate culture fluid channels from tissue chambers, featuring flexibly designable multitissue chambers. PC membranes act as capillary vertical direction, enabling precise hydrogel patterning successfully environment suitable growth. demonstrates ability build different shapes such triangle, rectangle, inverted triangle on more 10 days. cultured 12 days were perfused 70-kDa fluorescein isothiocyanate, which indicated generated had good barrier properties. A correlation between chamber shape shear stress was demonstrated using COMSOL, preliminary validation flow direction interstitial important effect growth by vascularization experiments. This flexible scalable is ideal culturing multiple vascularized organ tissues chip, well studying effects factors

Язык: Английский

Процитировано

2

Recent Advances and Future Perspectives in Vascular Organoids and Vessel-on-Chip DOI Creative Commons

Gowtham Reddy Cheruku,

Chloe Veronica Wilson,

Suriya Raviendran

и другие.

Organoids, Год журнала: 2024, Номер 3(3), С. 203 - 246

Опубликована: Сен. 4, 2024

Recent advancements in vascular organoid (VO) and vessel-on-chip (VoC) technologies have revolutionized our approach to studying human diseases, offering unprecedented insights through more physiologically relevant models. VOs generated from pluripotent stem cells exhibit remarkable self-organization capabilities, forming complex three-dimensional structures that closely mimic blood vessel architecture function, while VoCs are engineered with microfluidic systems meticulously recreate the physical functional attributes of vessels. These innovative constructs serve as powerful tools for investigating development, disease progression, therapeutic efficacy. By enabling creation patient-specific VoCs, they pave way personalized medicine approaches, allowing researchers delve into genetic variations, intricate cellular interactions, dynamic processes exceptional resolution. The synergy between newly developed cutting-edge has further amplified their potential, unveiling novel mechanisms underlying pathologies identifying promising targets. Herein, we summarize different types present an extensive overview on generation applications VoCs. We will also highlight clinical translational challenges future perspectives around

Язык: Английский

Процитировано

3

Advancing tissue engineering through vascularized cell spheroids: building blocks of the future DOI
Chunxiang Lü, Aoxiang Jin, Huazhen Liu

и другие.

Biomaterials Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

This review provides a comprehensive overview of vascularized cell spheroids, highlighting recent advancements in biofabrication, key strategies for engineering vascular networks, and their applications tissue models regenerative medicine.

Язык: Английский

Процитировано

0

Developments and Applications of Liver-on-a-Chip Technology—Current Status and Future Prospects DOI Creative Commons
Joseph Mugaanyi, Jing Huang, Jiongze Fang

и другие.

Biomedicines, Год журнала: 2025, Номер 13(6), С. 1272 - 1272

Опубликована: Май 22, 2025

Background/Objectives: Liver-on-a-chip (LiOC) technology is increasingly recognized as a transformative platform for modeling liver biology, disease mechanisms, drug metabolism, and toxicity screening. Traditional two-dimensional (2D) in vitro models lack the complexity needed to replicate liver’s unique microenvironment. This review aims summarize recent advancements LiOC systems, emphasizing their potential biomedical research translational applications. Methods: narrative synthesizes findings from key studies on development application of platforms. We explored innovations material science bioengineering, including microfluidic design, 3D printing, stem cell– tissue-derived organoid integration, co-culture strategies. Commercially available systems regulatory relevance were also evaluated. Results: have evolved simple PDMS-based chips complex, multicellular constructs incorporating hepatocytes, endothelial cells, Kupffer hepatic stellate cells. Recent demonstrate superior ability liver-specific architecture functions. Applications span cancer research, assessment (e.g., drug-induced injury prediction with >85% sensitivity), modeling, regenerative medicine. Several platforms gained FDA recognition are active use preclinical testing. Conclusions: offers more physiologically relevant alternative traditional holds promise reducing reliance animal studies. While challenges remain, such vascularization long-term function, ongoing paving way toward clinical pharmaceutical integration. The poised play role personalized medicine next-generation therapeutic development.

Язык: Английский

Процитировано

0

Engineering Cardiobundles with Dynamic Microenvironmental Control as Advanced Cardiac Modeling for Heart‐on‐a‐Chip DOI
Lanlan Hu,

Mingying Han,

Jiaxin Yang

и другие.

Small, Год журнала: 2025, Номер unknown

Опубликована: Май 26, 2025

Abstract Advancing cardioprotective therapies requires physiologically relevant preclinical models, yet conventional approaches—including 2D cell cultures, 3D spheroids, and animal models—fail to fully recapitulate the structural complexity dynamic microenvironment of native cardiac tissue, often facing species‐specific discrepancies ethical concerns. Organ‐on‐a‐chip (OoC) technology, a microfluidic‐based biomimetic system, offers real‐time organ‐level physiology disease progression monitoring. In this study, heart‐on‐a‐chip (HoC) platform is developed by integrating cardiobundles—engineered tissues—with microfluidic technology. Using fibrinogen‐Matrigel hydrogel aligned cardiobundles are fabricated that mimic myocardial architecture function. The HoC system demonstrates enhanced cardiomyocyte differentiation, intercellular connectivity, calcium handling, contractility. Furthermore, effects oxygen nutrient circulation, as well microenvironmental conditions, systematically assessed on cardiobundle performance, highlighting platform's physiological relevance. This represents promising tool for drug screening, modeling, personalised medicine, offering alternative traditional models in research.

Язык: Английский

Процитировано

0

Light-Based 3D Bioprinting Techniques for Illuminating the Advances of Vascular Tissue Engineering DOI Creative Commons
Wei Li, Jinhua Li, Chen Pan

и другие.

Materials Today Bio, Год журнала: 2024, Номер 29, С. 101286 - 101286

Опубликована: Окт. 2, 2024

Vascular tissue engineering faces significant challenges in creating

Язык: Английский

Процитировано

2

Recent Advances and Future Perspectives in Vascular Organoids and Vessel-on-Chip DOI Open Access

Gowtham Reddy Cheruku,

Chloe Veronica Wilson,

Suriya Raviendran

и другие.

Опубликована: Июль 14, 2024

Recent advancements in vascular organoid (VO) and vessel-on-chip (VoC) technologies have revolutionized our approach to studying cardiovascular diseases (CVDs), offering unprecedented insights through more physiologically relevant models. VOs generated from human pluripotent stem cells exhibit remarkable self-organization capabilities, forming complex three-dimensional structures that closely mimic blood vessel architecture function, while VoCs engineered with groundbreaking microfluidic systems meticulously recreate the physical functional attributes of vessels. These innovative constructs serve as powerful tools for investigating development, disease progression, therapeutic efficacy. By enabling creation patient-specific VoCs, they pave way personalized medicine approaches, allowing researchers delve into genetic variations, intricate cellular interactions, dynamic processes exceptional resolution. The synergy between cutting-edge such single-cell sequencing high-resolution imaging has further amplified their potential, unveiling novel mechanisms underlying CVDs identifying promising targets. Herein, we summarize different types present an extensive overview on generation applications CVDs. We will also highlight clinical translational challenges future perspectives around VoCs.

Язык: Английский

Процитировано

1

Arched Microfluidic Channel for the Promotion of Axonal Growth Performance DOI Creative Commons
Menghua Liu, Anping Wu, Jiaxin Liu

и другие.

iScience, Год журнала: 2024, Номер 27(10), С. 110885 - 110885

Опубликована: Сен. 4, 2024

Uniformly distributed fluid shear stress can promote axonal growth, aiding in the efficient construction of functional neural interfaces. However, challenges remain micro-scale environment with a uniform fluidic distribution. In this study, we designed and fabricated microfluidic chip arched-section channels (AMCs) to increase primary cortical neuron growth rate terminal number by constructing uniform-stress-distributed environment. Inspired three-dimensional (3D) microenvironment where cerebrospinal-fluid-contacting neurons are located, surface curvature traditional rectangular-section channel (RMC) was adjusted construct structures 3D curved surfaces. Compared those on RMC chips, average axons AMC chips increased 8.9% within 19 days, terminals 14.9%. This platform provides structure that effectively has potential more complex

Язык: Английский

Процитировано

0