Programmed Transformation of Osteogenesis Microenvironment by a Multifunctional Hydrogel to Enhance Repair of Infectious Bone Defects

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 22, 2025

Abstract Repair of infectious bone defects remains a serious problem in clinical practice owing to the high risk infection and excessive reactive oxygen species (ROS) during early stage, residual bacteria delayed Osseo integrated interface later which jointly creates complex dynamic microenvironment leads non‐union. The melatonin carbon dots (MCDs) possess antibacterial osteogenesis abilities, greatly simplifying composition multifunctional material. Therefore, hydrogel containing MCDs (GH‐MCD) is developed meet multi‐stage repair needs injury this study. GH‐MCD can intelligently release responding acidic scavenge intracellular ROS exhibit good activity by inducing production inhibiting expression secA2 . Moreover, it has long‐lasting antimicrobial repair. RNA‐seq results reveal that hydrogels promote infected healing enhancing cellular resistance bacteria, balancing osteoclastogenesis, regulating immune microenvironment. In conclusion, through programmed transformation microenvironment, providing novel strategy for defects.

Язык: Английский

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Thermoresponsive Injectable Microsphere Glycopeptide Hydrogels for Remodeling Dynamic Cell Microenvironments

ACS Applied Polymer Materials, Год журнала: 2025, Номер unknown

Опубликована: Янв. 24, 2025

Язык: Английский

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Progress in Procalcitonin Detection Based on Immunoassay

Research, Год журнала: 2024, Номер 7

Опубликована: Янв. 1, 2024

Procalcitonin (PCT) serves as a crucial biomarker utilized in diverse clinical contexts, including sepsis diagnosis and emergency departments. Its applications extend to identifying pathogens, assessing infection severity, guiding drug administration, implementing theranostic strategies. However, current deployed methods cannot meet the needs for accurate or real-time quantitative monitoring of PCT. This review aims introduce these emerging PCT immunoassay technologies, focusing on analyzing their advantages improving detection performances, such easy operation high precision. The fundamental principles characteristics state-of-the-art are first introduced, chemiluminescence, immunofluorescence, latex-enhanced turbidity, enzyme-linked immunosorbent, colloidal gold immunochromatography, radioimmunoassay. Then, improved using new materials technologies briefly described, instance, combination with responsive nanomaterials, Raman spectroscopy, digital microfluidics. Finally, performance parameters importance also discussed.

Язык: Английский

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Downregulation of the PI3K/AKT/mTOR/MMP-13 Pathway for Promoting Interface Healing via Lubricating Microspheres

Acta Biomaterialia, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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A 177Lu-nucleotide Coordination Polymer-incorporated Thermosensitive Hydrogel with Anti-inflammatory and Chondroprotective Capabilities for Osteoarthritis Treatment

Biomaterials, Год журнала: 2025, Номер 317, С. 123098 - 123098

Опубликована: Янв. 9, 2025

Язык: Английский

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Application and progress of smart hydrogel microspheres for regulating oxidative stress in osteoarthritis

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 160620 - 160620

Опубликована: Фев. 1, 2025

Язык: Английский

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Mitochondrial biogenesis microcarriers break membrane barriers for endogenous nucleus pulposus repair

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 160668 - 160668

Опубликована: Фев. 1, 2025

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Lubrication for Osteoarthritis: From Single-Function to Multifunctional Lubricants

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 1856 - 1856

Опубликована: Фев. 21, 2025

Osteoarthritis (OA) is a common degenerative joint disease that progressively destroys articular cartilage, leading to increased friction and severe pain. Therefore, OA can be treated by restoring the lubricating properties of cartilage. In this study, recent advances in lubricants for treatment are reviewed both single-function multifunctional lubricants. Single-function mainly include glycosaminoglycans, lubricin, phospholipids, whereas composed anti-inflammatory bifunctional hydrogels, stem cell-loaded drug-loaded nanoparticles. This review emphasizes importance lubrication capacity explores structural features, properties, role these modulating intracellular inflammatory responses metabolism. Current challenges future research directions field also discussed, with aim providing scientific basis new ideas clinical OA.

Язык: Английский

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Cerium Dioxide Nanoparticles‐Based Inspector Enhances Mitochondrial Quality Control to Maintain Chondrocyte Homeostasis in Osteoarthritis Therapy

Advanced Healthcare Materials, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

Abstract Imbalanced mitochondrial quality control is strongly linked to the onset and development of osteoarthritis (OA). However, current research primarily focuses on local cartilage repair phenotype maintenance, lacking a systematic approach subcellular control. To address this, present study proposes strategy based nanozyme hydrogel microspheres (“mitochondrial inspector”), constructed through electrostatic self‐assembly, incorporation dynamic diselenide bonds, microfluidic technology. The oxidative stress microenvironment improved by cerium dioxide nanoparticles combined with metformin activate autophagy clear persistently dysfunctional mitochondria, thereby inhibiting OA progression. In vitro results showed that “mitochondrial inspector” not only significantly chondrocytes, but also efficiently scavenged damaged increased membrane potential over 20‐fold, notably function chondrocyte homeostasis. rat model, minimally invasive intra‐articular injection effectively regulated quality, alleviated matrix degradation, reduced osteophyte formation ≈80%, Mankin score for damage 70%. summary, this presents novel microsphere‐based treatment OA, providing new insights therapies other aging‐related diseases.

Язык: Английский

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β‐Diketone Functionalized Microspheres Chelate Reactive Iron via Metal Coordination for Cartilage Repair

Advanced Healthcare Materials, Год журнала: 2025, Номер unknown

Опубликована: Март 5, 2025

Excessive intracellular iron accumulation can induce mitochondrial dysfunction, leading to chondrocyte ferroptosis, a key contributor cartilage damage in osteoarthritis (OA). Here, micelle-microfluidic hydrogel microspheres, featuring keto-enol-thiol bridged nano-sized secondary structures that disintegrate within the peroxidative environment reveal β-diketone groups with metal chelation capabilities, are utilized for situ removal of reactive iron, thereby facilitating repair through restoration homeostasis. The relevant experiments demonstrate microspheres reduce influx by downregulating transferrin receptor (TfR1) expression and decrease uptake upregulating outer membrane iron-sulfur cluster protein (CISD1), thus restoring Furthermore, antioxidant properties ketone-thioether segments synergistically mitigate phospholipid peroxidation via Nrf2/SLC7A11/GPX4 axis, inhibiting ferroptosis slowing OA progression. In summary, this system sustainably chelates coordination exhibits great potential minimally invasive treatment other ferroptosis-mediated diseases.

Язык: Английский

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