Journal of Materials Chemistry B, Год журнала: 2024, Номер 12(27), С. 6668 - 6677
Опубликована: Янв. 1, 2024
A nanoparticle-enhanced aptamer-recognizing homogeneous detection system combined with a portable instrument (NASPI) to quantify lipoarabinomannan (LAM) harnessing the synergistic interplay between fructose and histone.
Язык: Английский
Small Methods, Год журнала: 2025, Номер unknown
Опубликована: Март 22, 2025
Abstract An ultrasensitive ICP‐MS aptasensor is developed utilizing a label‐free, simple filter membrane‐assisted separation technique combined with nucleic acid signal amplification for the analysis of circulating tumor cells (CTCs) in lung cancer clinical samples. The approach based on high‐affinity interaction between aptamers and PD‐L1 mucin 1, which are overexpressed cell surface, conjunction functional Y‐DNA nanospheres catalytic hairpin assembly amplifications, enabling simultaneous detection two proteins. Additionally, four‐armed nanostructure significant spatial site resistance self‐assembled by introducing streptavidin biotinylated‐hairpin structures, improving efficiency membrane. This structural design enables effective isolation biotin‐T‐Hg 2+ ‐T biotin‐C‐Ag + ‐C from free Hg Ag , facilitating highly sensitive dual‐protein via ICP‐MS. limits reached ag mL −1 levels proteins single‐cell A549 cells. CTCs extracted whole blood samples patients within 45 min through centrifugation procedure. Quantification performed 37 samples, demonstrating results consistent diagnoses. assay exhibits specificity 100% sensitivity 94.5%.
Язык: Английский
Процитировано
1
Nanoscale, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 12, 2024
Nucleic acids have enabled the fabrication of self-assemblies and dynamic operations.
Язык: Английский
Процитировано
4
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Фев. 18, 2025
Circular tumor DNA (ctDNA) is a trace nucleic acid that functions as an essential marker. In this context, the present study proposes one-pot electrochemical analysis of ctDNA EGFR L858R in lung cancer leveraging Ag+-mediated nanosphere (I amplification) and cation exchange reaction (II amplification), Cu2+ acts signal molecule. Once target exists, it specifically destroys structure nanosphere@Ag+, large amounts Ag+ are released. After addition copper sulfide nanoparticles, can be replaced by reaction. Eventually, elevated. The analytical performance method satisfactory, detected linear range 1 aM-1 fM with detection limit 0.3 aM. Furthermore, system exhibits notable selectivity differentiating base mismatch targets other sequences. recovery rate blood samples between 95.5 105%. results from 42 clinical consistent those quantitative real-time polymerase chain reaction, computed tomography, pathology results. summary, novel strategy utilizes preprepared functional nanomaterials cascade amplification, which expected to contribute sensitive expeditious acids.
Язык: Английский
Процитировано
0
Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 162192 - 162192
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Coordination Chemistry Reviews, Год журнала: 2025, Номер 535, С. 216673 - 216673
Опубликована: Апрель 3, 2025
Язык: Английский
Процитировано
0
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Апрель 18, 2025
Liquid biopsy is an appealing approach for early diagnosis and assessment of treatment efficacy in cancer. Typically, liquid involves the detection endogenous biomarkers, including circulating tumor cells (CTCs), extracellular vesicles (EVs), DNA (ctDNA), RNA (ctRNA), proteins. The levels these biomarkers are higher cancer patients compared to those healthy individuals. However, clinical application using biomarker analysis faces challenges due its low abundance poor stability circulation. Recently, a promising strategy involving engineering exogenous probes has been developed overcome limitations. These activated within microenvironment, generating distinct markers that can be easily distinguished from background biological signals. Alternatively, labeled with intrinsic vivo detected vitro after metabolic processes. In this review, we primarily focus on microfluidic-based techniques allow transition analyzing existing emerging ones. First, introduce common as well synthetic Next, discuss recent advancements both biomarkers. Lastly, provide insights into future directions microfluidic systems.
Язык: Английский
Процитировано
0
Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 164315 - 164315
Опубликована: Май 1, 2025
Язык: Английский
Процитировано
0
Analytical Chemistry, Год журнала: 2024, Номер 96(26), С. 10705 - 10713
Опубликована: Июнь 24, 2024
Circulating tumor cells (CTCs) serve as important biomarkers in the liquid biopsy of hepatocellular carcinoma (HCC). Herein, a homogeneous dual fluorescence indicators aptasensing strategy is described for CTCs HCC, with core assistance steric hindrance-mediated enzymatic reaction. sample could specifically bind to 5'-biotin-modified glypican-3 (GPC3) aptamer and remove hindrance formed by biotin-streptavidin system. This influences efficiency terminal deoxynucleotidyl transferase Then, methylene blue (MB) was introduced react main product poly cytosine (polyC) chain, trivalent cerium ion (Ce
Язык: Английский
Процитировано
2
Sensors and Actuators B Chemical, Год журнала: 2024, Номер unknown, С. 136772 - 136772
Опубликована: Окт. 1, 2024
Язык: Английский
Процитировано
2
Journal of Materials Chemistry B, Год журнала: 2024, Номер 12(27), С. 6668 - 6677
Опубликована: Янв. 1, 2024
A nanoparticle-enhanced aptamer-recognizing homogeneous detection system combined with a portable instrument (NASPI) to quantify lipoarabinomannan (LAM) harnessing the synergistic interplay between fructose and histone.
Язык: Английский
Процитировано
0