The effect of the method of production a new fertilizer containing Streptomyces coelicolor grown on whey on its effectiveness

VEGETABLE CROPS OF RUSSIA, Год журнала: 2025, Номер 1, С. 108 - 113

Опубликована: Янв. 17, 2025

Relevance. In recent years, we have seen an increase in the number of new bacterial fertilizers. New fertilizers must be effective, so it is important to select their qualitative and quantitative composition. It equally study effect these bacteria on number, dissemination long-term residence soil, as well effects plants. Aim Study. This work studies method obtaining a fertilizer containing Streptomyces coelicolor grown whey amount duration soil growth characteristics white mustard ( Sinapis alba L.) sown into after long time watering with fertilizer. Methods. The was diluted autoclaved, were this substrate. An inoculum for biofertilizer obtained two ways. first way: once Waksman`s media. second sequentially whey, gradually increasing volume. Bacteria cultivated without mixing or by temperature-controlled shaker. We studied containers laboratory. determined 0.5, 1.5, 2 4 months Seeds mustard. Results. cultivating growing streptomyces contained ten times more than another method. also effectively increased soil. from been preserved time. biofertilizers most effectively. Conclusion. contributes effective then other methods. Our crops can used indoor crop production greenhouse farms.

Язык: Английский

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Exploring the composition and function profiles of bacteria from wood- and soil-feeding termites for effective degradation of lignin-based aromatics

Process Safety and Environmental Protection, Год журнала: 2024, Номер unknown

Опубликована: Сен. 1, 2024

Язык: Английский

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Complete Whole-Genome Sequence of Streptomyces sp. MUM 178J, a Potential Anti-Vibrio Agent

Progress In Microbes & Molecular Biology, Год журнала: 2024, Номер 7(1)

Опубликована: Фев. 16, 2024

Streptomyces sp. is a group of filamentous, Gram-positive bacteria notoriously known for their capabilities in producing bioactive compounds that have been used as novel drugs and lead drug development. The MUM 178J was isolated from mangrove forest Malaysia. This isolate found to harbor anti-Vibrio properties its crude extract inhibited the growth multidrug-resistant Vibrio parahaemolyticus. Therefore, strain subjected whole genome sequencing unearth genomic potential. consists 6,699,249 bp with G+C content 71.3%. 66 tRNA genes 18 rRNA were also predicted be present within genome. Further analysis bioinformatics tool, antiSMASH (antibiotics & Secondary Metabolite Analysis Shell), detected nine biosynthetic gene clusters displaying more than 70% similarity clusters, including one associated melanin production. Melanin has demonstrated antagonistic activity against members family, V. indicates potential correlation between production 178J. availability sequence allows future in-depth investigation exploitation harvest useful compounds.

Язык: Английский

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Functional connexion of bacterioferritin in antibiotic production and morphological differentiation in Streptomyces coelicolor

Microbial Cell Factories, Год журнала: 2024, Номер 23(1)

Опубликована: Авг. 24, 2024

Several two-component systems of Streptomyces coelicolor, a model organism used for studying antibiotic production in Streptomyces, affect the expression bfr (SCO2113) gene that encodes bacterioferritin, protein involved iron storage. In this work, we have studied effect deletion mutant ∆bfr S. coelicolor. The exhibits delay morphological differentiation and produces lesser amount two pigmented antibiotics (actinorhodin undecylprodigiosin) compared to wild type on complex media. minimal medium was tested mutant. Consequently, also observed different levels between strains, depending concentration (solid or liquid) used. Contrary expectations, no differences intracellular were detected However, higher level reactive oxygen species tolerance oxidative stress observed. Proteomic analysis showed variation response proteins, but there lower abundance proteins related actinorhodin ribosomal as well others secondary metabolite differentiation. Additionally, various types stress, such respiration hypoxia among others, revealed. Data are available via ProteomeXchange with identifier PXD050869. This bacterioferritin coelicolor (Bfr) is new element regulation metabolism and, additionally, acts signal modulate biosynthesis active molecules. Our proposes an interaction Bfr iron-containing regulatory proteins. Thus, identifying these interactions would provide information improving Streptomyces.

Язык: Английский

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The Role of Human Gut Microbe Ruminococcus gnavus in Inflammatory Diseases

Progress In Microbes & Molecular Biology, Год журнала: 2023, Номер 6(1)

Опубликована: Дек. 29, 2023

Dysbiosis is a prominent factor in numerous inflammatory conditions, with Ruminococcus gnavus, prevalent gut microbe, implicated bowel disease (IBD), irritable syndrome (IBS), eczema, metabolic disorders, and rheumatic diseases. R. gnavus contributes to inflammation by producing an polysaccharide degrading mucin. The increasing evidence of elevated abundance various conditions suggests its potential as therapeutic target. Despite this, there are limited studies on treatment strategies targeting gnavus. This article provides comprehensive overview the association between diverse highlighting need for further research explore effective options against this bacterium.

Язык: Английский

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Bacterial Exopolysaccharides: From Production to Functional Features

Progress In Microbes & Molecular Biology, Год журнала: 2023, Номер 6(1)

Опубликована: Ноя. 2, 2023

Exopolysaccharides, known as bacterial EPS, are complex sugar polymers that bacteria secrete into their environment. EPSs play a crucial role in survival and proliferation by protecting cells from environmental threats. They also contribute to proper adhesion numerous surfaces through biofilm production. Bacterial EPS display wide range of biological activities conferred outstanding physical chemical properties, which make them great candidates for medical industrial applications. The main recorded up-to-day include antioxidant, anticancer, antiviral, anti-inflammatory, antibacterial, immune-modulatory chelating properties. can be used thickening agents the industry or additives improve soil quality agriculture. current review offers thorough overview biosynthesis processes regulation, biotechnological strategies increase unravels extraction, purification, identification techniques highlights key functional features these molecules.

Язык: Английский

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Navigating the Role and Approach of Gut Microbiota in Addressing Alzheimer's Disease Pathogenesis

Progress In Microbes & Molecular Biology, Год журнала: 2024, Номер 7(1)

Опубликована: Ноя. 29, 2024

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder characterized by amyloid beta plaques and tau protein neurofibrillary tangles, leading to cognitive decline. The lack of effective treatments compounds the significant human financial burdens AD poses. Despite extensive research, exact mechanisms remain elusive. Recent studies have shown promise in using anti-Aβ antibodies reduce accumulation slow dementia progression. However, diversifying therapeutic strategies crucial for making meaningful progress. In recent years, research has increasingly focused on microbiota-gut-brain axis AD. Mounting evidence suggests that changes gut microbiota composition are linked progression, implicating various pathways. Dysregulation taxa can trigger systemic inflammation increasing permeability, ultimately neural damage neurodegeneration. Poor dietary habits aging exacerbate dysbiosis, worsening pathology. investigations this area still their early stages, with many aspects awaiting exploration understanding. A thorough comprehension complex interactions within microbiota-AD relationship essential refining approaches. Interventions targeting microbiota, such as adjustments, probiotics, faecal transplantation, offer potential therapeutics. This review highlights detrimental role dysbiosis AD, offering insights into enhancing avenues disease. Graphical abstract: bidirectional interaction between brain through neuroendocrine, immune, metabolic Created BioRender.com

Язык: Английский

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Detection of multidrug resistant Vibrio parahaemolyticus and anti-Vibrio Streptomyces sp. MUM 178J

Progress In Microbes & Molecular Biology, Год журнала: 2023, Номер 6(1)

Опубликована: Окт. 23, 2023

The growing global population has increased the demand for seafood, making aquaculture industry a vital food source. However, climate change negatively impacted as natural inhabitants of aquatic environments such Vibrio parahaemolyticus are thriving in warming waters. These foodborne pathogens can cause disease marine animals, resulting lowered production rates and substantial economic losses. V. is transmitted from these animals to humans via raw or undercooked gastroenteritis outbreaks. In addition, extensive use antibiotics treat infections within led development multidrug-resistant (MDR) parahaemolyticus, which threatens effectiveness antimicrobial treatments. Thus, it explore alternatives manage spread antibiotic resistance. This study aimed investigate prevalence MDR provide insight into current resistance patterns this pathogen determine potential anti-Vibrio properties Streptomyces MUM 178J was previously isolated soils mangrove forest Sarawak, Malaysia. Colony morphology toxR-assay indicated that all samples tested positive parahaemolyticus. Further identification with genomic analyses confirmed 64% (64/100) isolates were multiple index (MAR) ranged between 0.07 0.57, 81% (52/64) resistant more than one antibiotic. most ampicillin (98.4%), followed by ceftazidime (53.1%). contrast, highly susceptible nalidixic acid tetracycline (93.8%), sulfamethoxazole/trimethoprim (90.6%). They also demonstrated susceptibilities 89.1% levofloxacin, oxytetracycline, gentamicin. sp. exhibited antagonistic effects on select strain, RP0132, minimum inhibitory concentration 12.5 mg/mL bactericidal 50 mg/mL.

Язык: Английский

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Complete Whole Genome Sequence of Vibrio parahaemolyticus RP0132 Strain Isolated from Shrimp in Malaysia

Progress In Microbes & Molecular Biology, Год журнала: 2023, Номер 6(1)

Опубликована: Дек. 7, 2023

Vibrio parahaemolyticus is a Gram-negative, halophilic bacterium that ubiquitous in marine environments. Its perilous co-existence with aquatic animals increases the risk of infections and diseases, especially those aquaculture systems, thus resulting reduced production economic losses industry. Moreover, V. can be easily transmitted to humans via consumption contaminated seafood, gastroenteritis outbreaks. However, rise multidrug resistance within species has challenged efficacy antimicrobial treatments against infections. Therefore, we report genome sequence RP0132 isolated from shrimp gain insight into its traits potential mechanisms. The findings will facilitate development effective anti-Vibrio agents manage

Язык: Английский

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Newly Streptomyces-Induced Rabelomycin after Specific Amino Acid Supplementations: Experimental and Computational Investigations of Reactivities

Waste and Biomass Valorization, Год журнала: 2024, Номер 15(12), С. 6679 - 6692

Опубликована: Июнь 20, 2024

Язык: Английский

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