ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown
Опубликована: Фев. 12, 2025
Acute ischemic stroke (AIS) is associated with a high mortality rate and poor prognosis, lack of effective therapeutic drugs for post-thrombolytic treatment. MicroRNA-based gene therapy promising approach treating AIS, but its clinical application has been limited due to challenges, such as targeting efficiency, unsatisfactory stability, inadequate cellular uptake. In this study, we successfully developed microRNA-targeted delivery system based on the tetrahedral framework nucleic acid (tFNA). This nanodelivery system, guided by stroke-homing peptides, effectively targeted delivered miRNA124 hemisphere. With assistance tFNA, efficiently entered cells exerted effects. Additionally, it promoted transformation microglia from pro-inflammatory M1 phenotype anti-inflammatory M2 phenotype, reducing neuronal apoptosis and, ultimately, decreasing infarct size rate. These findings present strategy treatment AIS.
Язык: Английский