Perspectives on the Drosophila melanogaster Model for Advances in Toxicological Science

Current Protocols, Год журнала: 2023, Номер 3(8)

Опубликована: Авг. 1, 2023

The use of Drosophila melanogaster for studies toxicology has grown considerably in the last decade. model long been appreciated as a versatile and powerful developmental biology genetics because its ease handling, short life cycle, low cost maintenance, molecular genetic accessibility, availability wide range publicly available strains data resources. These features, together with recent unique developments genomics metabolomics, make fly especially relevant timely development new approach methodologies movements toward precision toxicology. Here, we offer perspective on how flies can be leveraged to identify risk factors environmental exposures human health. First, review discuss fundamental toxicologic principles experimental design Drosophila. Next, describe quantitative systems approaches resolve architecture candidate pathways controlling susceptibility toxicants. Finally, summarize current state future promise emerging field metabolomics elaborating toxic mechanisms. © 2023 Authors. Current Protocols published by Wiley Periodicals LLC.

Язык: Английский

A high-throughput method for quantifying Drosophila fecundity

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 30, 2024

Abstract Measurements of Drosophila fecundity are used in a wide variety studies, such as investigations stem cell biology, nutrition, behavior, and toxicology. In addition, because assays performed on live flies, they suitable for longitudinal studies aging or prolonged chemical exposure. However, standard have been difficult to perform high-throughput manner experimental factors the physiological state flies environmental cues must be carefully controlled achieve consistent results. exposing large number different conditions (such additives diet) manually counting eggs laid determine impact is time-consuming. We overcome these challenges by combining new multiwell fly culture strategy with novel 3D-printed transfer device rapidly accurately from one plate another; RoboCam, low-cost, custom built robotic camera capture images wells automatically; an image segmentation pipeline automatically identify quantify eggs. show that this method compatible robust egg laying throughout assay period; demonstrate automated quantifying very accurate (r 2 = 0.98 correlation between counts ground truth) we can efficiently detect effects induced dietary exposure chemicals. Taken together, substantially increases efficiency reproducibility high throughput require multiple media conditions.

Язык: Английский