Mounting Drosophila pupae for laser ablation and live imaging of the dorsal thorax

STAR Protocols, Год журнала: 2022, Номер 3(2), С. 101396 - 101396

Опубликована: Май 14, 2022

This protocol describes the preparation of Drosophilamelanogaster pupae for laser ablation and live imaging notum (dorsal thorax). Because pupa is stationary, it can be continuously imaged multiple days if desired, making ideal studying wound signaling repair, from before through closure. In this protocol, we demonstrate processes staging, partially dissecting, mounting, wounding, pupal notum, with wounding occurring during process. For complete details on use execution please refer to O'Connor et al. (2021b).

Язык: Английский

The utility of Drosophila melanogaster as a fungal infection model

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Март 14, 2024

Invasive fungal diseases have profound effects upon human health and are on increase globally. The World Health Organization (WHO) in 2022 published the priority list calling for improved public interventions advance research. Drosophila melanogaster presents an excellent model system to dissect host-pathogen interactions has been proved valuable study immunopathogenesis of diseases. In this review we highlight recent advances fungal- interplay with emphasis recently WHO’s focus available tools technologies.

Язык: Английский

Wounding increases nuclear ploidy in wound-proximal epidermal cells of the

PubMed, Год журнала: 2024, Номер 2024

Опубликована: Янв. 1, 2024

After injury, tissues must replace cell mass and genome copy number. The mitotic cycle is one mechanism for replacement, but non-mitotic strategies have been observed in quiescent to restore tissue ploidy after wounding. Here we report that nuclei of the mitotically capable

Язык: Английский