Identification and Evaluation of Hub Long Non-Coding RNAs and mRNAs in PM2.5-Induced Lung Cell Injury

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 911 - 911

Опубликована: Янв. 22, 2025

Exposure to air pollution, especially fine particulate matter (PM2.5), is closely linked various adverse health effects, particularly in the respiratory system. The present study was designed investigate lncRNA–mRNA interactions PM2.5-induced lung cell injury using weighted gene co-expression network analysis (WGCNA). We downloaded expression data of GSE138870 from Gene Expression Omnibus (GEO) database and screened for differentially expressed lncRNAs mRNAs. constructed modules with WGCNA. Furthermore, functional enrichment also performed. networks lncRNA–mRNA-pathway identify key regulatory relationships. results revealed several significantly correlated injury, such as turquoise blue modules. Genes within these were enriched pathways related signal transduction, metabolism, cancer. Hub module, including LOC100129034 CROCCP2, found be co-expressed mRNAs involved apoptosis proliferation regulation. In lnc-CLVS2-2 GARS1-DT connected genes migration, invasion, injury. These findings contribute novel perspectives molecular mechanisms suggest that WGCNA could a valuable tool predicting understanding this disease process.

Язык: Английский

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Identification and Assessment of lncRNAs and mRNAs in PM2.5-Induced Hepatic Steatosis

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2808 - 2808

Опубликована: Март 20, 2025

Research indicates that fine particulate matter (PM2.5) exposure is associated with the onset of non-alcoholic fatty liver disease (NAFLD), most prevalent chronic disorder. However, underlying pathogenesis mechanisms remain to be fully understood. Our study investigated hub long non-coding RNAs (lncRNAs) and messenger (mRNAs) hepatic steatosis caused by PM2.5 their pathological mechanisms. The analysis gene profiles in GSE186900 dataset from Gene Expression Omnibus (GEO) enabled identification 38 differentially expressed lncRNAs 1945 mRNAs. To explore further, a co-expression network was established utilizing weighted (WGCNA). Moreover, Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) databases were utilized for functional enrichment analysis. identified specific modules, particularly blue turquoise which showed strong correlation NAFLD. Through analysis, we several (including Gm15446, Tmem181b-ps, Adh6-ps1, Gm5848, Zfp141, Rmrp, Rb1) may involved modulating NAFLD, multiple metabolic pathways, inflammation, cell senescence, apoptosis, oxidative stress, various signaling pathways. our provide novel biomarkers potential targets diagnosis treatment

Язык: Английский

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Diagnostic and Predictive Value of LncRNA MCM3AP-AS1 in Sepsis and Its Regulatory Role in Sepsis-Induced Myocardial Dysfunction

Cardiovascular Toxicology, Год журнала: 2024, Номер 24(10), С. 1125 - 1138

Опубликована: Июль 31, 2024

Язык: Английский

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Bidirectional modulation of extracellular vesicle-autophagy axis in acute lung injury: Molecular mechanisms and therapeutic implications

Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 180, С. 117566 - 117566

Опубликована: Окт. 17, 2024

Язык: Английский

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The Diagnostic Utility of Host RNA Biosignatures in Adult Patients With Sepsis: A Systematic Review and Meta-Analysis

Critical Care Explorations, Год журнала: 2025, Номер 7(2), С. e1212 - e1212

Опубликована: Янв. 31, 2025

OBJECTIVES: Sepsis is a life-threatening medical emergency, with profound healthcare burden globally. Its pathophysiology complex, heterogeneous and temporally dynamic, making diagnosis challenging. Medical management predicated on early timely intervention. Transcriptomics one of the novel “-omics” technologies being evaluated for recognition sepsis. Our objective was to evaluate performance host gene expression biosignatures all-cause sepsis in adults. DATA SOURCES: PubMed/Ovid Medline, Ovid Embase, Cochrane databases from inception June 2023. STUDY SELECTION: We included studies evaluating adults who were diagnosed using existing clinical definitions. Controls where applicable patients without EXTRACTION: Data including population demographics, sample size, study design, tissue specimen, type transcriptome, health status comparator group, transcriptomic biomarkers independently extracted by at least two reviewers. SYNTHESIS: Meta-analysis describe adult performed random-effects model. Risk bias assessed according Quality Assessment Diagnostic Accuracy Studies-2 tool. A total 117 ( n = 17,469), comprising 132 separate patient datasets, our final analysis. Performance transcriptomics against pooled controls showed area under receiver operating characteristic curve (AUC, 0.86; 95% CI, 0.84–0.88). Studies healthy AUC 0.87 (95% 0.84–0.89), while systemic inflammatory response syndrome (SIRS) had 0.84 0.78–0.90). Transcripts excellent discrimination SIRS include UrSepsisModel, 210 differentially expressed genes biosignature, microRNA-143, Septicyte laboratory. CONCLUSIONS: promising approach accurate demonstrates good discriminatory ability both control subjects.

Язык: Английский

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The Role of lncRNAs in Sepsis-Induced Acute Lung Injury: Molecular Mechanisms and Therapeutic Potential

Archives of Biochemistry and Biophysics, Год журнала: 2025, Номер unknown, С. 110407 - 110407

Опубликована: Апрель 1, 2025

Язык: Английский

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LncRNA H19 alleviates sepsis-induced acute lung injury by regulating the miR-107/TGFBR3 axis

BMC Pulmonary Medicine, Год журнала: 2022, Номер 22(1)

Опубликована: Сен. 30, 2022

Abstract Objective Acute lung injury (ALI) increases sepsis morbidity and mortality. LncRNA H19 plays a critical role in sepsis. miR-107 is highly-expressed TGFβ type III receptor (TGFBR3) poorly-expressed sepsis, yet their roles development require further investigation. This study aimed to investigate the mechanism of alleviating sepsis-induced ALI through miR-107/TGFBR3 axis. Methods Mice were intravenously injected with Ad-H19 adenovirus vector or control one week before establishing mouse model cecal ligation puncture (CLP). Pulmonary microvascular endothelial cells (PMVECs) transfected oe-H19 oe-NC plasmids then stimulated by lipopolysaccharide (LPS). Lung was assessed via hematoxylin–eosin staining, measurement wet-to-dry (W/D) ratio, TUNEL staining. Levels H19, miR-107, TGFBR3 determined RT-qPCR. Apoptosis PMVECs evaluated flow cytometry. Bax Bcl-2 tissues measured using Western blot. Total protein concentration number total cells, neutrophils, macrophages bronchoalveolar lavage fluid (BALF) quantified. TNF-α, IL-1β, IL-6, IL-10 BALF, tissues, ELISA. Cross-linking relationships among verified dual-luciferase RIP assays. Results CLP-operated mice. overexpression attenuated ALI, which manifested complete alveolar structure, decreased score W/D inhibited apoptosis mice, TUNEL-positive level increased level. mice had nullified overexpression. declined levels IL-6 elevated levels. LPS-induced PMVEC pro-inflammatory cytokine production. targeted as ceRNA miR-107. silencing partially averted inhibition on Conclusion production targeting

Язык: Английский

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Regulatory role of noncoding RNA in sepsis and sepsis-associated organ dysfunction: an updated systematic review

Shock, Год журнала: 2022, Номер unknown

Опубликована: Сен. 26, 2022

Background: The exact molecular mechanisms underlying sepsis remain unclear. Accumulating evidence has shown that noncoding RNAs (ncRNAs) are involved in and sepsis-associated organ dysfunction (SAOD). Methods: We performed this updated systematic review focusing mainly on research conducted the last 5 years regarding ncRNAs associated with SAOD. following medical subject headings were used PubMed database from October 1, 2016, to March 31, 2022: "microRNA," "long RNA," "circular "sepsis," and/or "septic shock." Studies investigating role of pathogenesis as biomarkers or therapeutic targets disease included. Data extracted terms their applicability for use sepsis. quality studies was assessed using a modified guideline Systematic Review Center Laboratory Animal Experimentation. Results: A total 537 original investigated potential roles Experimental confirmed long have important regulatory However, circular limited, more should be elucidate relationship. Among included studies, Experimentation scores ranged 3 7 (an average score 3.78). Notably, 94 evaluated sepsis, selective reporting sensitivity, specificity, receiver operating characteristic curve common. 117 demonstrated At level, inflammation-related pathways, mitochondrial dysfunction, cell apoptosis, oxidative stress most extensively studied. Conclusion: This suggests could good candidates Prospective, large-scale, multicenter cohort evaluate specific test organ-specific delivery these molecules when targets.

Язык: Английский

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Exosomes as novel biomarkers in sepsis and sepsis related organ failure

Journal of Translational Medicine, Год журнала: 2024, Номер 22(1)

Опубликована: Ноя. 28, 2024

Sepsis, a severe and life-threatening condition arising from dysfunctional host response to infection, presents considerable challenges the health care system is characterized by high mortality rates substantial economic costs. Exosomes have garnered attention as potential diagnostic markers because of their capacity mirror pathophysiological milieu sepsis. This discourse reviews progression sepsis classification Sepsis 1.0 3.0, highlighting imperative for sensitive specific biomarkers facilitate timely diagnosis optimize patient outcomes. Existing biomarkers, such procalcitonin (PCT) C-reactive protein (CRP), exhibit certain limitations, thereby prompting quest more dependable indicators. Exosomal cargoes, which encompass proteins miRNAs, present trove biomarker candidates, attributable stability, pervasive presence, indicative nature disease status. The exosomal in identification sepsis-induced organ damage, including cardiomyopathy, acute kidney injury, lung emphasized, they provide real-time insights into cardiac renal impairments. Despite promising prospects, hurdles persist standardization exosome extraction need extensive clinical trials validate efficacy. combination development sophisticated detection techniques represents pioneering strategy realm management, underscoring significance further research validation.

Язык: Английский

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Long noncoding RNA MALAT1 inhibition attenuates sepsis‐induced acute lung injury through modulating the miR‐129‐5p/PAX6/ZEB2 axis

Microbiology and Immunology, Год журнала: 2022, Номер 67(3), С. 142 - 153

Опубликована: Дек. 20, 2022

This research aimed to investigate the role of long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1)/microRNA-129-5p (miR-129-5p)/paired box gene 6 (PAX6) axis in sepsis-induced acute injury (ALI). MLE-12 cells and C57BL/6 mice were induced by LPS establish vitro vivo models. Cell viability apoptosis measured cell counting kit-8 assay TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) staining, respectively. Levels inflammatory cytokines supernatants bronchoalveolar lavage fluid (BALF) detected ELISA. Lung was evaluated wet weight-to-dry weight ratio hematoxylin-eosin staining. MALAT1, PAX6, zinc finger E-box-binding homeobox 2 (ZEB2) expression elevated miR-129-5p reduced serum patients with ALI, LPS-induced cells, tissues ALI mice. MALAT1 interference delayed proliferation decrease, increase, factor increase. inhibition could reverse delaying effect on injury. PAX6 overexpression (oe) reversed inhibitory oe Downregulating pulmonary edema, cytokine levels, injury, Moreover, suppression or ALI. aggravates via miR-129-5p/PAX6/ZEB2 axis.

Язык: Английский

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