Life,
Год журнала:
2024,
Номер
14(12), С. 1688 - 1688
Опубликована: Дек. 20, 2024
Aquaporins
(AQPs)
are
membrane
proteins
facilitating
water
and
other
small
solutes
to
be
transported
across
cell
membranes.
They
crucial
in
maintaining
cellular
homeostasis
by
regulating
permeability
various
tissues.
Moreover,
they
regulate
migration,
signaling
pathways,
inflammation,
tumor
growth,
metastasis.
In
critically
ill
patients,
such
as
trauma,
sepsis,
patients
with
acute
respiratory
distress
syndrome
(ARDS),
which
frequently
encountered
intensive
care
units
(ICUs),
transport
regulation
is
for
homeostasis,
dysregulation
can
lead
edema
or
dehydration,
the
latter
also
implicating
hemodynamic
compromise.
Indeed,
AQPs
involved
fluid
organs,
including
lungs,
kidneys,
brain,
where
their
dysfunction
exacerbate
conditions
like
ARDS,
kidney
injury
(AKI),
cerebral
edema.
this
review,
we
discuss
implication
of
clinical
entities
ICUs,
systemic
inflammation
AKI,
brain
due
different
types
primary
from
a
perspective.
Current
possible
future
therapeutic
implications
considered.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(3), С. 911 - 911
Опубликована: Янв. 22, 2025
Exposure
to
air
pollution,
especially
fine
particulate
matter
(PM2.5),
is
closely
linked
various
adverse
health
effects,
particularly
in
the
respiratory
system.
The
present
study
was
designed
investigate
lncRNA–mRNA
interactions
PM2.5-induced
lung
cell
injury
using
weighted
gene
co-expression
network
analysis
(WGCNA).
We
downloaded
expression
data
of
GSE138870
from
Gene
Expression
Omnibus
(GEO)
database
and
screened
for
differentially
expressed
lncRNAs
mRNAs.
constructed
modules
with
WGCNA.
Furthermore,
functional
enrichment
also
performed.
networks
lncRNA–mRNA-pathway
identify
key
regulatory
relationships.
results
revealed
several
significantly
correlated
injury,
such
as
turquoise
blue
modules.
Genes
within
these
were
enriched
pathways
related
signal
transduction,
metabolism,
cancer.
Hub
module,
including
LOC100129034
CROCCP2,
found
be
co-expressed
mRNAs
involved
apoptosis
proliferation
regulation.
In
lnc-CLVS2-2
GARS1-DT
connected
genes
migration,
invasion,
injury.
These
findings
contribute
novel
perspectives
molecular
mechanisms
suggest
that
WGCNA
could
a
valuable
tool
predicting
understanding
this
disease
process.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(6), С. 2808 - 2808
Опубликована: Март 20, 2025
Research
indicates
that
fine
particulate
matter
(PM2.5)
exposure
is
associated
with
the
onset
of
non-alcoholic
fatty
liver
disease
(NAFLD),
most
prevalent
chronic
disorder.
However,
underlying
pathogenesis
mechanisms
remain
to
be
fully
understood.
Our
study
investigated
hub
long
non-coding
RNAs
(lncRNAs)
and
messenger
(mRNAs)
hepatic
steatosis
caused
by
PM2.5
their
pathological
mechanisms.
The
analysis
gene
profiles
in
GSE186900
dataset
from
Gene
Expression
Omnibus
(GEO)
enabled
identification
38
differentially
expressed
lncRNAs
1945
mRNAs.
To
explore
further,
a
co-expression
network
was
established
utilizing
weighted
(WGCNA).
Moreover,
Ontology
(GO)
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
databases
were
utilized
for
functional
enrichment
analysis.
identified
specific
modules,
particularly
blue
turquoise
which
showed
strong
correlation
NAFLD.
Through
analysis,
we
several
(including
Gm15446,
Tmem181b-ps,
Adh6-ps1,
Gm5848,
Zfp141,
Rmrp,
Rb1)
may
involved
modulating
NAFLD,
multiple
metabolic
pathways,
inflammation,
cell
senescence,
apoptosis,
oxidative
stress,
various
signaling
pathways.
our
provide
novel
biomarkers
potential
targets
diagnosis
treatment
Critical Care Explorations,
Год журнала:
2025,
Номер
7(2), С. e1212 - e1212
Опубликована: Янв. 31, 2025
OBJECTIVES:
Sepsis
is
a
life-threatening
medical
emergency,
with
profound
healthcare
burden
globally.
Its
pathophysiology
complex,
heterogeneous
and
temporally
dynamic,
making
diagnosis
challenging.
Medical
management
predicated
on
early
timely
intervention.
Transcriptomics
one
of
the
novel
“-omics”
technologies
being
evaluated
for
recognition
sepsis.
Our
objective
was
to
evaluate
performance
host
gene
expression
biosignatures
all-cause
sepsis
in
adults.
DATA
SOURCES:
PubMed/Ovid
Medline,
Ovid
Embase,
Cochrane
databases
from
inception
June
2023.
STUDY
SELECTION:
We
included
studies
evaluating
adults
who
were
diagnosed
using
existing
clinical
definitions.
Controls
where
applicable
patients
without
EXTRACTION:
Data
including
population
demographics,
sample
size,
study
design,
tissue
specimen,
type
transcriptome,
health
status
comparator
group,
transcriptomic
biomarkers
independently
extracted
by
at
least
two
reviewers.
SYNTHESIS:
Meta-analysis
describe
adult
performed
random-effects
model.
Risk
bias
assessed
according
Quality
Assessment
Diagnostic
Accuracy
Studies-2
tool.
A
total
117
(
n
=
17,469),
comprising
132
separate
patient
datasets,
our
final
analysis.
Performance
transcriptomics
against
pooled
controls
showed
area
under
receiver
operating
characteristic
curve
(AUC,
0.86;
95%
CI,
0.84–0.88).
Studies
healthy
AUC
0.87
(95%
0.84–0.89),
while
systemic
inflammatory
response
syndrome
(SIRS)
had
0.84
0.78–0.90).
Transcripts
excellent
discrimination
SIRS
include
UrSepsisModel,
210
differentially
expressed
genes
biosignature,
microRNA-143,
Septicyte
laboratory.
CONCLUSIONS:
promising
approach
accurate
demonstrates
good
discriminatory
ability
both
control
subjects.
BMC Pulmonary Medicine,
Год журнала:
2022,
Номер
22(1)
Опубликована: Сен. 30, 2022
Abstract
Objective
Acute
lung
injury
(ALI)
increases
sepsis
morbidity
and
mortality.
LncRNA
H19
plays
a
critical
role
in
sepsis.
miR-107
is
highly-expressed
TGFβ
type
III
receptor
(TGFBR3)
poorly-expressed
sepsis,
yet
their
roles
development
require
further
investigation.
This
study
aimed
to
investigate
the
mechanism
of
alleviating
sepsis-induced
ALI
through
miR-107/TGFBR3
axis.
Methods
Mice
were
intravenously
injected
with
Ad-H19
adenovirus
vector
or
control
one
week
before
establishing
mouse
model
cecal
ligation
puncture
(CLP).
Pulmonary
microvascular
endothelial
cells
(PMVECs)
transfected
oe-H19
oe-NC
plasmids
then
stimulated
by
lipopolysaccharide
(LPS).
Lung
was
assessed
via
hematoxylin–eosin
staining,
measurement
wet-to-dry
(W/D)
ratio,
TUNEL
staining.
Levels
H19,
miR-107,
TGFBR3
determined
RT-qPCR.
Apoptosis
PMVECs
evaluated
flow
cytometry.
Bax
Bcl-2
tissues
measured
using
Western
blot.
Total
protein
concentration
number
total
cells,
neutrophils,
macrophages
bronchoalveolar
lavage
fluid
(BALF)
quantified.
TNF-α,
IL-1β,
IL-6,
IL-10
BALF,
tissues,
ELISA.
Cross-linking
relationships
among
verified
dual-luciferase
RIP
assays.
Results
CLP-operated
mice.
overexpression
attenuated
ALI,
which
manifested
complete
alveolar
structure,
decreased
score
W/D
inhibited
apoptosis
mice,
TUNEL-positive
level
increased
level.
mice
had
nullified
overexpression.
declined
levels
IL-6
elevated
levels.
LPS-induced
PMVEC
pro-inflammatory
cytokine
production.
targeted
as
ceRNA
miR-107.
silencing
partially
averted
inhibition
on
Conclusion
production
targeting
Background:
The
exact
molecular
mechanisms
underlying
sepsis
remain
unclear.
Accumulating
evidence
has
shown
that
noncoding
RNAs
(ncRNAs)
are
involved
in
and
sepsis-associated
organ
dysfunction
(SAOD).
Methods:
We
performed
this
updated
systematic
review
focusing
mainly
on
research
conducted
the
last
5
years
regarding
ncRNAs
associated
with
SAOD.
following
medical
subject
headings
were
used
PubMed
database
from
October
1,
2016,
to
March
31,
2022:
"microRNA,"
"long
RNA,"
"circular
"sepsis,"
and/or
"septic
shock."
Studies
investigating
role
of
pathogenesis
as
biomarkers
or
therapeutic
targets
disease
included.
Data
extracted
terms
their
applicability
for
use
sepsis.
quality
studies
was
assessed
using
a
modified
guideline
Systematic
Review
Center
Laboratory
Animal
Experimentation.
Results:
A
total
537
original
investigated
potential
roles
Experimental
confirmed
long
have
important
regulatory
However,
circular
limited,
more
should
be
elucidate
relationship.
Among
included
studies,
Experimentation
scores
ranged
3
7
(an
average
score
3.78).
Notably,
94
evaluated
sepsis,
selective
reporting
sensitivity,
specificity,
receiver
operating
characteristic
curve
common.
117
demonstrated
At
level,
inflammation-related
pathways,
mitochondrial
dysfunction,
cell
apoptosis,
oxidative
stress
most
extensively
studied.
Conclusion:
This
suggests
could
good
candidates
Prospective,
large-scale,
multicenter
cohort
evaluate
specific
test
organ-specific
delivery
these
molecules
when
targets.
Journal of Translational Medicine,
Год журнала:
2024,
Номер
22(1)
Опубликована: Ноя. 28, 2024
Sepsis,
a
severe
and
life-threatening
condition
arising
from
dysfunctional
host
response
to
infection,
presents
considerable
challenges
the
health
care
system
is
characterized
by
high
mortality
rates
substantial
economic
costs.
Exosomes
have
garnered
attention
as
potential
diagnostic
markers
because
of
their
capacity
mirror
pathophysiological
milieu
sepsis.
This
discourse
reviews
progression
sepsis
classification
Sepsis
1.0
3.0,
highlighting
imperative
for
sensitive
specific
biomarkers
facilitate
timely
diagnosis
optimize
patient
outcomes.
Existing
biomarkers,
such
procalcitonin
(PCT)
C-reactive
protein
(CRP),
exhibit
certain
limitations,
thereby
prompting
quest
more
dependable
indicators.
Exosomal
cargoes,
which
encompass
proteins
miRNAs,
present
trove
biomarker
candidates,
attributable
stability,
pervasive
presence,
indicative
nature
disease
status.
The
exosomal
in
identification
sepsis-induced
organ
damage,
including
cardiomyopathy,
acute
kidney
injury,
lung
emphasized,
they
provide
real-time
insights
into
cardiac
renal
impairments.
Despite
promising
prospects,
hurdles
persist
standardization
exosome
extraction
need
extensive
clinical
trials
validate
efficacy.
combination
development
sophisticated
detection
techniques
represents
pioneering
strategy
realm
management,
underscoring
significance
further
research
validation.