Unraveling the role of CRX as a potent intrinsic suppressor of epithelial-mesenchymal transition in retinal pigment epithelial cells
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 2, 2025
Abstract
The
epithelial-mesenchymal
transition
(EMT)
of
retinal
pigment
epithelial
(RPE)
cells
is
one
the
significant
pathogenic
mechanisms
for
formation
subretinal
fibrosis
in
age-related
macular
degeneration
(AMD).
Multiple
signaling
pathways
that
promote
EMT
have
been
well
described,
yet
endogenous
inhibit
within
RPE
remain
largely
elusive.
In
this
study,
we
confirmed
expression
CRX
human
and
embryonic
stem
cell-derived
(ESC-RPE)
cells.
By
employing
sub-culture
to
disrupt
intercellular
connections
thereby
Hippo
pathway,
combined
with
TGF-β1
treatment
vitro
mimic
microenvironment
fibrosis,
it
was
revealed
Hippo/YAP1
synergistically
promoted
nuclear
translocation
β-catenin,
latter
bound
TCF7
CRX.
Overexpression
capable
suppressing
occurrence
ESC-RPE
exerted
its
inhibitory
effect
on
partly
by
upregulating
PPP2R2B.
laser-induced
choroidal
neovascularization
mouse
model,
took
place
cells,
overexpression
played
an
role
fibrosis.
This
study
has
identified
as
molecule
inhibits
provided
a
new
research
target
strategy
wet
AMD
inhibition
formation.
Язык: Английский
Metformin inhibits subretinal fibrosis by activating Klotho by miR-126-5p
Zhijuan Hua,
Qin Zhu,
Jingfei Yang
и другие.
Cytotechnology,
Год журнала:
2025,
Номер
77(3)
Опубликована: Апрель 2, 2025
Язык: Английский