Unraveling the metabolic‒epigenetic nexus: a new frontier in cardiovascular disease treatment
Cell Death and Disease,
Год журнала:
2025,
Номер
16(1)
Опубликована: Март 18, 2025
Abstract
Cardiovascular
diseases
are
the
leading
causes
of
death
worldwide.
However,
there
still
shortcomings
in
currently
employed
treatment
methods
for
these
diseases.
Therefore,
exploring
molecular
mechanisms
underlying
cardiovascular
is
an
important
avenue
developing
new
strategies.
Previous
studies
have
confirmed
that
metabolic
and
epigenetic
alterations
often
involved
across
patients.
Moreover,
factors
interact
with
each
other
affect
progression
a
coordinated
manner.
Lactylation
novel
posttranslational
modification
(PTM)
links
metabolism
epigenetics
affects
disease
progression.
analyzing
crosstalk
between
cellular
expected
to
provide
insights
development
The
purpose
this
review
describe
relationship
heart
such
as
failure,
myocardial
infarction,
atherosclerosis,
focus
on
acylation
methylation,
propose
potential
therapeutic
measures.
Язык: Английский
Targeting exercise-related genes and placental growth factor for therapeutic development in head and neck squamous cell carcinoma
Qingyuan Shi,
Haiyue Ying,
Weibin Weng
и другие.
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Окт. 4, 2024
Background
Human
cancers,
including
head
and
neck
squamous
cell
carcinoma
(HNSCC),
are
complex
heterogeneous
diseases
driven
by
uncontrolled
growth
proliferation.
Post-translational
modifications
(PTMs)
of
proteins
play
a
crucial
role
in
cancer
progression,
making
them
promising
target
for
pharmacological
intervention.
This
study
aims
to
identify
key
exercise-related
genes
with
prognostic
value
HNSCC
through
comprehensive
bioinformatics
analysis,
particular
focus
on
the
therapeutic
potential
placental
factor
(PIGF).
Methods
Transcriptome
data
were
obtained
from
The
Cancer
Genome
Atlas
(TCGA)
database.
Differently
expressed
(DEGs)
identified
analyzed
their
significance.
Exercise-related
gene
sets
retrieved
Gene
Set
Enrichment
Analysis
(GSEA)
Functional
enrichment
analyses,
Ontology
(GO),
Kyoto
Encyclopedia
Genes
Genomes
(KEGG),
GSEA,
conducted.
biological
functions
clinical
implications
further
explored
single-gene
expression
immune
infiltration
vitro
cellular
experiments.
Results
associated
survival
prognosis
HNSCC.
GO
KEGG
pathway
analyses
highlighted
these
genes,
Kaplan-Meier
curves
confirmed
value.
PIGF
analysis
using
TCGA
showed
its
diagnostic
potential,
higher
linked
advanced
tumor
stages.
Single-cell
sequencing
revealed
PIGF’s
microenvironment.
In
experiments
demonstrated
that
plays
pivotal
enhancing
proliferation
colony
formation
HNSCC,
knockdown
significantly
impairing
functions,
highlighting
importance
regulation.
Additionally,
predictive
performance
drug
sensitivity
across
datasets
suggests
as
target,
offering
opportunities
modulate
microenvironment
improve
outcomes
treatment.
Conclusion
provides
new
insights
into
molecular
mechanisms
underlying
identifies
particularly
PIGF,
biomarkers
treatment
personalized
medicine.
By
focusing
PTMs
our
findings
suggest
targeting
may
offer
innovative
strategies.
Язык: Английский
Paromomycin targets HDAC1-mediated SUMOylation and IGF1R translocation in glioblastoma
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Дек. 11, 2024
Objective
This
study
investigates
the
effects
of
Paromomycin
on
SUMOylation-related
pathways
in
glioblastoma
(GBM),
specifically
targeting
HDAC1
inhibition.
Methods
Using
TCGA
and
GTEx
datasets,
we
identified
genes
associated
with
GBM
prognosis.
Molecular
docking
analysis
suggested
as
a
potential
inhibitor.
In
vitro
assays
U-251MG
cells
were
performed
to
assess
Paromomycin’s
cell
viability,
SUMOylation
gene
expression,
IGF1R
translocation
using
CCK8
assays,
qRT-PCR,
immunofluorescence.
Results
treatment
led
dose-dependent
reduction
colony
formation,
migration.
It
modulated
SUMO1
expression
decreased
nuclear
translocation,
an
effect
reversible
by
inhibitor
Trochostatin
A
(TSA),
suggesting
involvement
SUMO1-regulated
pathways.
Conclusion
highlights
therapeutic
agent
for
HDAC1-mediated
influencing
warranting
further
investigation
its
clinical
application.
Язык: Английский
Cross-disease transcriptomic analysis reveals DOK3 and PAPOLA as therapeutic targets for neuroinflammatory and tumorigenic processes
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Дек. 12, 2024
Objective
Subarachnoid
hemorrhage
(SAH)
and
tumorigenesis
share
numerous
biological
complexities;
nevertheless,
the
specific
gene
expression
profiles
underlying
mechanisms
remain
poorly
understood.
This
study
aims
to
identify
differentially
expressed
genes
(DEGs)
that
could
serve
as
biomarkers
for
diagnosis
prognosis.
Methods
Gene
datasets
(GSE122063,
GSE13353,
GSE161870)
were
analyzed
using
machine
learning
algorithms
logistic
regression
DEGs
associated
with
both
SAH
tumorigenesis.
Lasso
receiver
operating
characteristic
(ROC)
curve
analysis
employed
evaluate
classification
accuracy
of
these
genes.
Validation
critical
was
performed
through
pan-cancer
experimental
studies,
focusing
on
role
DOK3
in
modulating
inflammation
oxidative
stress
U251MG
glioblastoma
BV2
microglia
cells.
Results
Fifteen
common
identified,
PAPOLA
highlighted
crucial
implicated
neurodegenerative
processes.
Experimental
validation
demonstrated
overexpression
significantly
reduced
pro-inflammatory
cytokine
levels
markers
while
enhancing
antioxidant
enzyme
activity.
Additionally,
influenced
tumorigenic
processes
such
apoptosis,
cell
cycle
regulation,
proliferation,
effectively
mitigating
LPS-induced
cytotoxicity
microglial
Conclusions
play
roles
related
neurodegeneration,
presenting
themselves
potential
prognostic
therapeutic
targets.
Notably,
exhibits
an
antitumor
agent
anti-inflammatory
antioxidative
properties,
offering
benefits
cancer
neuroinflammatory
conditions.
Язык: Английский