A Janus Amyloid-like Nanofilm Inhibits Colorectal Cancer Postoperative Recurrence and Abdominal Adhesion via Synergistic Enzyme Cascade DOI Creative Commons

Man Zhang,

Junhao Kou,

Ziwei Song

и другие.

Nanomaterials, Год журнала: 2025, Номер 15(9), С. 670 - 670

Опубликована: Апрель 28, 2025

Postoperative peritoneal adhesion and high recurrence rates are critical challenges in the clinical treatment of colorectal cancer. In this study, based on amyloid-like protein self-assembly technology, a novel Janus film was developed. The encapsulates glucose oxidase (GOx) catalase (CAT), which is named PTL@GC. Through one-step method involving cysteine-reduced lysozyme-induced self-assembly, co-loaded with GOx CAT to achieve synergistic anti-adhesion anti-tumor effects. features hydrophobic side that stably adheres intestinal surface without exogenous chemical modification hydrophilic prevents adhesion. loaded selectively induces disulfidptosis SLC7A11-overexpressing tumor cells, while degrades H2O2 alleviate hypoxia inhibit oxidative stress, significantly reducing adhesion-related fibrosis. experimental results demonstrate PTL@GC exhibited excellent mechanical properties, enzyme activity retention (>90%), controllable degradability (complete metabolism within 50 days). animal models, reduced postoperative area by 22.77%, decreased local burden 28.42% control group, achieved an inhibition rate 58.49%, inducing systemic toxicity. This study presents biologically safe functionally approach addressing dual complications following cancer surgery, offering potential insights for future research multifunctional materials.

Язык: Английский

A Janus Amyloid-like Nanofilm Inhibits Colorectal Cancer Postoperative Recurrence and Abdominal Adhesion via Synergistic Enzyme Cascade DOI Creative Commons

Man Zhang,

Junhao Kou,

Ziwei Song

и другие.

Nanomaterials, Год журнала: 2025, Номер 15(9), С. 670 - 670

Опубликована: Апрель 28, 2025

Postoperative peritoneal adhesion and high recurrence rates are critical challenges in the clinical treatment of colorectal cancer. In this study, based on amyloid-like protein self-assembly technology, a novel Janus film was developed. The encapsulates glucose oxidase (GOx) catalase (CAT), which is named PTL@GC. Through one-step method involving cysteine-reduced lysozyme-induced self-assembly, co-loaded with GOx CAT to achieve synergistic anti-adhesion anti-tumor effects. features hydrophobic side that stably adheres intestinal surface without exogenous chemical modification hydrophilic prevents adhesion. loaded selectively induces disulfidptosis SLC7A11-overexpressing tumor cells, while degrades H2O2 alleviate hypoxia inhibit oxidative stress, significantly reducing adhesion-related fibrosis. experimental results demonstrate PTL@GC exhibited excellent mechanical properties, enzyme activity retention (>90%), controllable degradability (complete metabolism within 50 days). animal models, reduced postoperative area by 22.77%, decreased local burden 28.42% control group, achieved an inhibition rate 58.49%, inducing systemic toxicity. This study presents biologically safe functionally approach addressing dual complications following cancer surgery, offering potential insights for future research multifunctional materials.

Язык: Английский

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