The role of N6-methyladenosine modification in tumor angiogenesis DOI Creative Commons
Qin Li,

Xinya Zeng,

Xinze Qiu

и другие.

Frontiers in Oncology, Год журнала: 2024, Номер 14

Опубликована: Дек. 3, 2024

Tumor angiogenesis is a characteristics of malignant cancer progression that facilitates cell growth, diffusion and metastasis, has an indispensable role in development. N6-methyladenosine (m6A) among the most prevalent internal modifications eukaryotic RNAs, considerable influence on RNA metabolism, including its transcription, splicing, localization, translation, recognition, degradation. The m6A modification generated by methyltransferases ("writers"), removed demethylases ("erasers"), recognized m6A-binding proteins ("readers"). There accumulating evidence abnormal involved pathogenesis multiple diseases, cancers, promotes occurrence, development, through impact oncoprotein expression. Furthermore, increasing studies have demonstrated can cancers pathways to regulate processes. In this review, we elaborate tumor angiogenesis-related molecules detail, providing insights into interactions between angiogenesis. Moreover, describe how targeting combination with anti-angiogenesis drugs expected be promising anti-tumor treatment strategy, potential value for addressing challenge drug resistance.

Язык: Английский

Transcriptomic analysis of Porphyromonas gingivalis‐infected head and neck cancer cells: Identification of PLAU as a candidate prognostic biomarker DOI Creative Commons
Masakazu Hamada, Hiroaki Inaba,

Kyoko Nishiyama

и другие.

Journal of Cellular and Molecular Medicine, Год журнала: 2024, Номер 28(4)

Опубликована: Фев. 1, 2024

Periodontal disease is a risk factor for head and neck squamous cell carcinoma (HNSCC), Porphyromonas gingivalis, major periodontal pathogen, has been identified as specific potentially independent microbial that increases the of cancer mortality. Gene expression in HNSCC due to P. gingivalis infection how changes gene affect prognosis patients are not clarified. When was cultured with cells, it efficiently adhered these cells enhanced their invasive ability. A transcriptome analysis -infected showed genes related migration, including CCL20, CITED2, CTGF, C8orf44-SGK3, DUSP10, EGR3, FUZ, HBEGF, IL1B, IL24, JUN, PLAU, PTGS2, P2RY1, SEMA7A, SGK1 SIX2, were highly up- or down-regulated. The up-regulated examined using data obtained from Cancer Genome Atlas (TCGA) database, 5 genes, found be higher tissue than solid normal tissue. An protein-protein interactions revealed formed dense network. Cox regression high PLAU levels associated poor TCGA-HNSCC. Furthermore, prognostic impact correlated tumour size presence absence lymph node metastasis. Collectively, results suggest potential molecular marker patients. Further vivo vitro studies needed verify findings this study.

Язык: Английский

Процитировано

4
Establishment of a Prognostic Model for Pancreatic Cancer Based on Hypoxia-Related Genes DOI Creative Commons
Yangdong Wu,

Jianrui Zhou,

Qingyan Kou

и другие.

Technology in Cancer Research & Treatment, Год журнала: 2024, Номер 23

Опубликована: Янв. 1, 2024

Objectives Pancreatic cancer presents a formidable challenge with its aggressive nature and dismal prognosis, often hampered by elusive early symptoms. The tumor microenvironment (TME) emerges as pivotal player in pancreatic progression treatment responses, characterized notably hypoxia immunosuppression. In this study, we aimed to identify hypoxia-related genes develop prognostic model for leveraging these genes. Methods Through analysis of gene expression data from Cancer Genome Atlas (TCGA) subsequent GO/KEGG enrichment analysis, pathways were identified. We constructed using lasso regression validated it an independent dataset. Results Our results showed that levels PLAU, SLC2A1, CA9 exhibited significant associations prognosis cancer. model, built upon genes, displayed robust predictive accuracy was Furthermore, found correlation between the risk score clinical parameters patients. At same time, also explored relationship established immune at single-cell level. RT-qPCR notable differences pathway-related normal PANC-1 hypoxic-treated cells. Conclusion study provides insights into role hypoxic offers promising tool application.

Язык: Английский

Процитировано

4
The roles of YY1 in reproductive system: an overview DOI
Yinan Wang, S. L. He, Qin Huang

и другие.

Chemico-Biological Interactions, Год журнала: 2025, Номер 417, С. 111560 - 111560

Опубликована: Май 15, 2025

Язык: Английский

Процитировано

0
Population-Specific gene expression profiles in prostate cancer: insights from Weighted Gene Co-expression Network Analysis (WGCNA) DOI Creative Commons

Laleh Manouchehri,

Zahra Zinati, Leyla Nazari

и другие.

World Journal of Surgical Oncology, Год журнала: 2024, Номер 22(1)

Опубликована: Июль 5, 2024

Abstract This study investigates the genetic factors contributing to disparity in prostate cancer incidence and progression among African American men (AAM) compared European (EAM). The research focuses on employing Weighted Gene Co-expression Network Analysis (WGCNA) public microarray data obtained from patients. employed WGCNA identify clusters of genes with correlated expression patterns, which were then analyzed for their connection population backgrounds. Additionally, pathway enrichment analysis was conducted understand significance identified gene modules pathways. Least Absolute Shrinkage Selection Operator (LASSO) Correlation-based Feature (CFS) methods utilized selection biomarker genes. results revealed 353 differentially expressed (DEGs) between AAM EAM. Six significant through WGCNA, showing varying degrees correlation cancer. LASSO CFS pinpointed critical genes, as well six common both approaches, are indicative vital role disease. XGBoost classifier validated these findings, achieving satisfactory prediction accuracy. Genes such APRT, CCL2, BEX2, MGC26963, PLAU key significantly associated progression. In conclusion, underlines importance incorporating EAM diversity genomic studies, particularly research. addition, highlights effectiveness integrating machine learning techniques a robust methodology identifying

Язык: Английский

Процитировано

3
Urokinase Plasminogen Activation System Modulation in Transformed Cell Lines DOI Open Access
Diana Culej,

Tihana Balent,

Petra Korać

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(2), С. 675 - 675

Опубликована: Янв. 15, 2025

The role of the plasminogen activation system is to regulate activity extracellular protease plasmin. It comprises urokinase activator (uPA), a specific which activates plasminogen, its inhibitor PAI1, and receptor, uPAR, localizes activity. involved in tissue remodeling through matrix degradation, therefore participates numerous physiological pathological processes, make it potential biomarker. To investigate these molecules cellular we cloned human uPA, uPAR overexpressed them two cell lines, glioblastoma line A1235 transformed embryonal kidney cells HEK 293. We analyzed expression elements on protein RNA level by Western blot analysis RTqPCR. Cell proliferation was followed up counting, migration invasion wound-healing transwell assays, respectively, adhesion dispersal spheroid formation. transfected with sequence had increased uPA expression, while PAI1-transfected decreased activity, PAI1 migration. 293 expressing PAI formed only small spheroids. effects depended their interactions each other microenvironment, as well cell-type-specific signaling.

Язык: Английский

Процитировано

0
Epigallocatechin Gallate Inhibits Cell Proliferation Promoted by TNFα/IL1β‐NFκB‐PLAU Inflammatory Signaling in Esophageal Squamous Cell Carcinoma DOI Creative Commons
Fei Zhou,

Yuanduo Li,

Yurui Zhang

и другие.

Journal of Food Biochemistry, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

Background: Proinflammatory cytokines TNFα and IL1β drive esophageal squamous cell carcinoma (ESCC) proliferation. However, the underlying molecular mechanism potential therapeutic interventions to target this inflammatory signaling remain unclear. Methods: Plasminogen activator urokinase ( PLAU ) expression was analyzed using public database (GEO iProX) experiments (qRT‐PCR Western blotting). The DNA‐binding activity of nuclear factor κB (NFκB) at promoter several online servers (AnimalTFDB, JASPAR, PROMO, Cistrome, UCSC) confirmed through ChIP‐qPCR. role in ESCC proliferation investigated overexpression experiments, GO annotation, CCK8 assay, 5‐ethynyl‐2′‐deoxyuridine (EdU) incorporation assay. Results: significantly higher tissues compared normal cells immortalized epithelial cells. Treatment with induced NFκB binding cells, leading increased expression. Conversely, treatment BAY11‐7082, an inhibitor, blocked upregulation. Overexpression promoted Thus, our findings demonstrate that TNFα/IL1β‐NFκB‐ axis promotes Moreover, EGCG inhibited promoter, thereby preventing upregulation TNFα/IL1β‐treated inhibiting by overexpression. Conclusion: effectively blocks TNFα/IL1β‐NFκB‐PLAU, Our study provides new insights into blocking pro‐proliferative inflammation highlights as a agent.

Язык: Английский

Процитировано

0
The Effects and Mechanisms of the YY1/EGFR Axis on Inflammation and Oxidative Stress in Asthma DOI
Yue Li, Pengfei Li,

Tianci Jiang

и другие.

Cell Biochemistry and Biophysics, Год журнала: 2025, Номер unknown

Опубликована: Апрель 4, 2025

Язык: Английский

Процитировано

0
Integrative bioinformatics and machine learning identify key crosstalk genes and immune interactions in head and neck cancer and Hodgkin lymphoma DOI Creative Commons

Meiling Qin,

Xinxin Li, Xun Gong

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Май 6, 2025

Язык: Английский

Процитировано

0
Exploring program-cell death patterns to predict prognosis and sensitivity of cervical cancer immunotherapy via multi-omics analysis and clinical samples DOI Creative Commons

Chunhong Pang,

Xianfeng Long,

Yongjin Luo

и другие.

Discover Oncology, Год журнала: 2025, Номер 16(1)

Опубликована: Май 28, 2025

Язык: Английский

Процитировано

0
Effects of anoxic prognostic model on immune microenvironment in pancreatic cancer DOI Creative Commons
Yangdong Wu,

Qingyan Kou,

Lin Sun

и другие.

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Июнь 5, 2023

Abstract Pancreatic cancer has one of the worst prognoses in world, which suggests that tumor microenvironment, is characterized by hypoxia and immunosuppression, plays a significant role prognosis progression pancreatic cancer. We identified PLAU, LDHA, PKM as key genes involved through GO/KEGG enrichment related pathways cox regression, established prognostic models, studied their relationship to immune invasion bioinformatics using R online databases. verified high expression cells qPCR vitro, we also discovered hypoxic differed from normal cultured cells. Finally, our model accurately predicted postrain patients with infiltration.

Язык: Английский

Процитировано

6