Association of Calpain-10 gene polymorphisms with Type 2 diabetes mellitus: a case-control study from a tertiary care hospital in Pakistan DOI Creative Commons
Humaira Farooqi,

Nakhshab Choudhry,

Muhammad Nabeel Saddique

и другие.

BMC Medical Genomics, Год журнала: 2025, Номер 18(1)

Опубликована: Май 19, 2025

Type 2 diabetes mellitus (T2DM) is a major public health challenge, with rising prevalence in low- and middle-income countries such as Pakistan. Genetic susceptibility plays critical role its pathogenesis. Calpain-10 (CAPN-10), gene implicated insulin secretion glucose homeostasis, has been studied for potential involvement T2DM. This study aimed to evaluate the association of CAPN-10 polymorphisms-SNP44 (rs2975760) SNP43 (rs3792267)-with T2DM Pakistani cohort. case-control included 164 patients healthy controls (mean age ± SD: 57.2 8.2 vs. 53.9 6.3 years; range: 41-82 years). The male-to-female ratio was 41.4-58.6% cases 37.2-62.8% controls. Participants were enrolled using non-probability convenience sampling. Genomic DNA extracted from whole blood, genotyping SNPs (rs3792267 rs2975760) performed PCR-RFLP. Genotype distributions assessed Hardy-Weinberg equilibrium. Associations evaluated odds ratios (ORs) 95% confidence intervals (CIs) via logistic regression. Chi-square tests used categorical comparisons, p < 0.05 considered statistically significant. Analyses conducted SPSS version 26. For SNP44, no significant observed under dominant, heterozygous, or recessive models after Bonferroni correction (adjusted > 0.05). Similarly, showed either dominant 0.05), although AA genotype appeared more frequently among cases. These findings suggest polymorphisms this population. SNP44 population, suggesting limited predictive value disease susceptibility.

Язык: Английский

Association of Calpain-10 Gene Polymorphisms with Type 2 Diabetes Mellitus: A Case-Control Study from a Tertiary Care Hospital in Pakistan DOI
Humaira Farooqi,

Nakhshab Choudhry,

Muhammad Nabeel Saddique

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Апрель 17, 2025

Abstract Introduction: Type 2 diabetes mellitus (T2DM) is a major public health challenge, with rising prevalence in low- and middle-income countries such as Pakistan. Genetic susceptibility plays critical role its pathogenesis. Calpain-10 (CAPN-10), gene implicated insulin secretion glucose homeostasis, has been studied for potential involvement T2DM. This study aimed to evaluate the association of CAPN-10 polymorphisms—SNP44 (rs2975760) SNP43 (rs3792267)—with T2DM Pakistani cohort. Methods: This case-control included 164 patients healthy controls (mean age ± SD: 57.2 8.2 vs. 53.9 6.3 years; range: 41–82 years). The male-to-female ratio was 41.4% 58.6% cases 37.2% 62.8% controls. Participants were enrolled using non-probability convenience sampling. Genomic DNA extracted from whole blood, genotyping SNPs (rs3792267 rs2975760) performed PCR-RFLP. Genotype distributions assessed Hardy-Weinberg equilibrium. Associations evaluated odds ratios (ORs) 95% confidence intervals (CIs) via logistic regression. Chi-square tests used categorical comparisons, p < 0.05 considered statistically significant. Analyses conducted SPSS version 26. Results: For SNP44, no significant observed under dominant, heterozygous, or recessive models after Bonferroni correction (adjusted > 0.05). Similarly, showed either dominant 0.05), although AA genotype appeared more frequently among cases. These findings suggest polymorphisms this population. Conclusion: SNP44 population, suggesting limited predictive value disease susceptibility.

Язык: Английский

Процитировано

0
Dysglycemia-Based Chronic Disease DOI Creative Commons

Michael A. Via,

Jeffrey I. Mechanick

Lifestyle Medicine, Год журнала: 2025, Номер unknown, С. 31 - 54

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Association of Calpain-10 gene polymorphisms with Type 2 diabetes mellitus: a case-control study from a tertiary care hospital in Pakistan DOI Creative Commons
Humaira Farooqi,

Nakhshab Choudhry,

Muhammad Nabeel Saddique

и другие.

BMC Medical Genomics, Год журнала: 2025, Номер 18(1)

Опубликована: Май 19, 2025

Type 2 diabetes mellitus (T2DM) is a major public health challenge, with rising prevalence in low- and middle-income countries such as Pakistan. Genetic susceptibility plays critical role its pathogenesis. Calpain-10 (CAPN-10), gene implicated insulin secretion glucose homeostasis, has been studied for potential involvement T2DM. This study aimed to evaluate the association of CAPN-10 polymorphisms-SNP44 (rs2975760) SNP43 (rs3792267)-with T2DM Pakistani cohort. case-control included 164 patients healthy controls (mean age ± SD: 57.2 8.2 vs. 53.9 6.3 years; range: 41-82 years). The male-to-female ratio was 41.4-58.6% cases 37.2-62.8% controls. Participants were enrolled using non-probability convenience sampling. Genomic DNA extracted from whole blood, genotyping SNPs (rs3792267 rs2975760) performed PCR-RFLP. Genotype distributions assessed Hardy-Weinberg equilibrium. Associations evaluated odds ratios (ORs) 95% confidence intervals (CIs) via logistic regression. Chi-square tests used categorical comparisons, p < 0.05 considered statistically significant. Analyses conducted SPSS version 26. For SNP44, no significant observed under dominant, heterozygous, or recessive models after Bonferroni correction (adjusted > 0.05). Similarly, showed either dominant 0.05), although AA genotype appeared more frequently among cases. These findings suggest polymorphisms this population. SNP44 population, suggesting limited predictive value disease susceptibility.

Язык: Английский

Процитировано

0