Sulforaphane regulates cell proliferation and induces apoptotic cell death mediated by ROS-cell cycle arrest in pancreatic cancer cells DOI Creative Commons
Yongmin Cho, Moon Nyeo Park, Min Ji Choi

и другие.

Frontiers in Oncology, Год журнала: 2024, Номер 14

Опубликована: Авг. 29, 2024

Background Pancreatic cancer (PC), sometimes referred to as pancreatic ductal adenocarcinoma (PDAC), is a major cause of global mortality from cancer. very aggressive and devastating kind cancer, characterized by limited options for therapy low possibilities survival. Sulforaphane (SFN), naturally occurring sulfur-containing compound, believed possess anti-inflammatory, anti-obesity, anti-cancer characteristics. Objective However, efficient preventative treatment measures are essential SFN has been studied its ability suppress cell proliferation induce apoptosis. Methods Here, induced cytotoxicity apoptosis in PDAC lines such MIA PaCa-2 PANC-1 cells, evaluated cytotoxicity, colony formation, western blot analysis, fluorescence-activated sorting (FACS), reactive oxygen species (ROS) detection, caspase-3 activity assay, immunofluorescence mitochondrial membrane potential assay. Results In inhibited survival dose-dependent manner. The activation caspase zymogens results cleaved PARP caspase-3, which associated with an accumulation the sub G1 phase. Furthermore, increased ROS level γH2A.X expression while decreasing (ΔΨm). Notably, scavenger N-Acetyl-L-cysteine (NAC) was shown reverse SFN-induced level. Subsequently, cycle arrest induction Trojan horse eliminate cells via ROS-mediated pathways were used inhibit cells. Conclusion Collectively, our data demonstrates that death follows pathway, making it viable target therapeutic interventions against

Язык: Английский

Overcoming immunotherapeutic drug resistance in pancreatic and colon cancers DOI

Mario Tavakoli,

Hasan Raza,

Dirin Ukwade

и другие.

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 305 - 324

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Sulforaphane regulates cell proliferation and induces apoptotic cell death mediated by ROS-cell cycle arrest in pancreatic cancer cells DOI Creative Commons
Yongmin Cho, Moon Nyeo Park, Min Ji Choi

и другие.

Frontiers in Oncology, Год журнала: 2024, Номер 14

Опубликована: Авг. 29, 2024

Background Pancreatic cancer (PC), sometimes referred to as pancreatic ductal adenocarcinoma (PDAC), is a major cause of global mortality from cancer. very aggressive and devastating kind cancer, characterized by limited options for therapy low possibilities survival. Sulforaphane (SFN), naturally occurring sulfur-containing compound, believed possess anti-inflammatory, anti-obesity, anti-cancer characteristics. Objective However, efficient preventative treatment measures are essential SFN has been studied its ability suppress cell proliferation induce apoptosis. Methods Here, induced cytotoxicity apoptosis in PDAC lines such MIA PaCa-2 PANC-1 cells, evaluated cytotoxicity, colony formation, western blot analysis, fluorescence-activated sorting (FACS), reactive oxygen species (ROS) detection, caspase-3 activity assay, immunofluorescence mitochondrial membrane potential assay. Results In inhibited survival dose-dependent manner. The activation caspase zymogens results cleaved PARP caspase-3, which associated with an accumulation the sub G1 phase. Furthermore, increased ROS level γH2A.X expression while decreasing (ΔΨm). Notably, scavenger N-Acetyl-L-cysteine (NAC) was shown reverse SFN-induced level. Subsequently, cycle arrest induction Trojan horse eliminate cells via ROS-mediated pathways were used inhibit cells. Conclusion Collectively, our data demonstrates that death follows pathway, making it viable target therapeutic interventions against

Язык: Английский

Процитировано

3