CNPY3’s regulation of tumor microenvironment and its impact on colon cancer aggressiveness DOI Creative Commons
Xucan Gao, Biaohuan Zhou, Xiaohui Feng

и другие.

Molecular Medicine, Год журнала: 2025, Номер 31(1)

Опубликована: Март 7, 2025

Abstract Background Canopy FGF signaling regulator 3 (CNPY3) has been implicated in tumor progression. However, its specific role colon cancer (CC) remains unclear. This study aims to investigate the function of CNPY3 CC and potential as a therapeutic target. Methods A total 201 tissue specimens 67 adjacent non-cancerous tissues were collected for analysis. expression was assessed using immunohistochemistry quantitative real-time PCR. Functional assays conducted cell lines (HT-29 SW-620) following knockdown evaluate effects on proliferation, migration, apoptosis. Gene profiling, fibroblast co-culture experiments, vivo xenograft models also conducted. Results Increased correlated with advanced stages poorer prognosis. Knockdown significantly inhibited induced apoptosis lines. depletion modulated behavior, inhibiting their transformation into cancer-associated fibroblasts. Pathway analysis revealed that affected cycle p53 pathways, reduced activation MAPK PI3K/AKT pathways. Additionally, enhanced sensitivity 5-fluorouracil. In studies demonstrated resulted smaller sizes weights than controls. Conclusions is crucial progression, correlating aggressiveness poor patient outcomes. Targeting may offer promising strategy valuable prognostic marker management.

Язык: Английский

Natural Alternatives in the Treatment of Colorectal Cancer: A Mechanisms Perspective DOI Creative Commons
Karen Vanessa Fernandez-Muñoz, Ángel Sánchez‐Barrera, Marco Antonio Meraz‐Ríos

и другие.

Biomolecules, Год журнала: 2025, Номер 15(3), С. 326 - 326

Опубликована: Фев. 24, 2025

Colorectal cancer (CRC) is one of the deadliest neoplasia. Intrinsic or acquired resistance main cause failure therapy regimens that leads to relapse and death in CRC patients. The widely used chemotherapeutic agent 5-fluorouracil (5-FU) remains mainstay for therapeutic combinations. Unfortunately, side effects are frequent events compromise success these therapies; dysregulation enzymes regulate 5-FU metabolism increases expression activity efflux pumps. Additional tumor cell adaptations such as epithelial–mesenchymal transition (EMT), autophagy shaping microenvironment, inflammation contribute chemoresistance. Finding new strategies alternatives enhance conventional chemotherapies has become necessary. Recently, study natural compounds been gaining strength an alternative chemotherapeutics different cancers. Curcumin, trimethylglycine, resveratrol, artemisinin, some helminth-derived molecules, among others, studied context CRC. This review discusses benefits, mechanisms, advances, dark currently evaluated treatment. We also analyzed landscape non-conventional their underlying mechanisms action, which could, short term, provide fundamental knowledge harness anti-tumor allow them be adjuvant therapies.

Язык: Английский

Процитировано

1

CNPY3’s regulation of tumor microenvironment and its impact on colon cancer aggressiveness DOI Creative Commons
Xucan Gao, Biaohuan Zhou, Xiaohui Feng

и другие.

Molecular Medicine, Год журнала: 2025, Номер 31(1)

Опубликована: Март 7, 2025

Abstract Background Canopy FGF signaling regulator 3 (CNPY3) has been implicated in tumor progression. However, its specific role colon cancer (CC) remains unclear. This study aims to investigate the function of CNPY3 CC and potential as a therapeutic target. Methods A total 201 tissue specimens 67 adjacent non-cancerous tissues were collected for analysis. expression was assessed using immunohistochemistry quantitative real-time PCR. Functional assays conducted cell lines (HT-29 SW-620) following knockdown evaluate effects on proliferation, migration, apoptosis. Gene profiling, fibroblast co-culture experiments, vivo xenograft models also conducted. Results Increased correlated with advanced stages poorer prognosis. Knockdown significantly inhibited induced apoptosis lines. depletion modulated behavior, inhibiting their transformation into cancer-associated fibroblasts. Pathway analysis revealed that affected cycle p53 pathways, reduced activation MAPK PI3K/AKT pathways. Additionally, enhanced sensitivity 5-fluorouracil. In studies demonstrated resulted smaller sizes weights than controls. Conclusions is crucial progression, correlating aggressiveness poor patient outcomes. Targeting may offer promising strategy valuable prognostic marker management.

Язык: Английский

Процитировано

0