Elevated expression of let-7b-3p enhances aggressiveness of larynx squamous cell carcinoma cells
Anatolian Current Medical Journal,
Год журнала:
2025,
Номер
7(1), С. 27 - 32
Опубликована: Янв. 10, 2025
Aims:
Larynx
squamous
cell
carcinoma
(LSCC)
is
the
second
most
common
head
and
neck
malignancy.
While
let-7b-3p
has
been
shown
to
have
a
role
in
cancer
progression
malignancies,
there
no
research
examining
association
between
LSCC
let-7b-3p.
This
study
aimed
investigate
expression
status
of
potential
roles
this
microRNA
(miRNA)
LSCC.
Methods:
Using
quantitative
real-time
polymerase
chain
reaction
(qRT-PCR),
we
examined
let-7b3p
36
samples
neighboring
normal
tissues.
Then,
miRNA
mimic
was
transfected
into
Hep-2
cells
via
lipofectamine
2000
reagents.
Cell
viability
determined
using
detection
(CVDK-8)
kit,
migration
evaluated
with
scratch
assay.
To
identify
differentially
expressed
genes
(DEGs)
larynx
GSE137308
GSE130605
datasets
were
downloaded
reanalyzed
Gene
Expression
Omnibus
(GEO2R)
tool.
Potential
target
investigated
prediction
functional
annotation
database
(miRDB).
Shared
geo
miRDB
results
identified
relationship
these
literature.
Results:
We
demonstrated
that
levels
significantly
upregulated
tumor
tissues
comparison
corresponding
Mimic
transfection
enhanced
proliferation
migration.
In
vitro
bioinformatics
analysis
showed
overexpression
can
enhance
through
MYBPC1.
Conclusion:
It
let-7b-3p/MYBPC1
axis
could
potentially
affect
process.
Let-7b-3p
be
biomarker
for
LSCC,
therefore,
let-7b-3p/
MYBPC1/LSCC
should
elucidated
new
studies.
Язык: Английский
Overexpression of CDC25A, AURKB, and TOP2A Genes Could Be an Important Clue for Luminal A Breast Cancer
Meme sağlığı dergisi/Meme sağlığı dergisi,
Год журнала:
2024,
Номер
unknown, С. 284 - 291
Опубликована: Сен. 26, 2024
Breast
cancer
(BC)
is
highly
heterogeneous
and
one
of
the
most
common
cancers.
Luminal
A
(LUM
A)
a
subtype
BC
with
better
prognosis
than
other
subtypes.
The
molecular
mechanisms
underlying
initiation
progression
LUM
are
still
unclear.
Big
data
generated
from
microarray
sequencing
systems
can
be
re-analyzed,
especially
help
various
Язык: Английский
CDR1as/miR-7-5p/IGF1R axis contributes to the suppression of cell viability in prostate cancer
Turkish Journal of Biochemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 5, 2024
Abstract
Background
Prostate
cancer
is
the
most
frequently
diagnosed
male
and
fifth
highest
cause
of
mortality
in
men.
CDR1as
has
played
an
essential
role
growth
several
malignancies.
However,
its
significance
progression
prostate
not
been
investigated.
We
aimed
to
investigate
mechanism
development
identify
a
new
target
for
diagnostics
treatment.
Methods
siRNA
miR-7-5p
mimic
were
transfected
into
PC3
DU145
PCa
cell
lines
their
effects
on
cellular
processes
Cell
viability
was
measured
by
WST-8
assay.
The
and/or
migration
detected
using
scratch-wound
apoptotic
capacity
cells
evaluated
Caspase-3
kit.
potential
targets
defined
via
silico
tools.
mRNA
protein
expression
levels
IGF1R
EIF4E
qRT-PCR
western
blot
assays,
respectively.
matching
between
luciferase
reporter
Results
Inhibiting
or
restoring
reduced
proliferation
while
increasing
apoptosis.
Silencing
elevated
decreasing
IGF1R.
Conclusions
functions
as
sponge,
promoting
tumor
development.
Язык: Английский
miRNA, phytometabolites and disease: Connecting the dots
Phytotherapy Research,
Год журнала:
2024,
Номер
38(9), С. 4570 - 4591
Опубликована: Июль 29, 2024
Abstract
miRNAs
are
tiny
noncoding
ribonucleotides
that
function
as
critical
regulators
of
gene‐expression
in
eukaryotes.
A
single
miRNA
may
be
involved
the
regulation
several
target
mRNAs
forming
complex
cellular
networks
to
regulate
diverse
aspects
development
an
organism.
The
deregulation
has
been
associated
with
human
diseases.
Therefore,
miRNA‐based
therapeutics
is
gaining
interest
pharmaceutical
industry
next‐generation
drugs
for
cure
many
Medicinal
plants
have
also
used
treatment
diseases
and
their
curative
potential
attributed
reserve
bioactive
metabolites.
role
phytometabolic
pathways
emerged
recent
past.
Experimental
studies
indicated
plant
encoded
secondary
phytometabolites
act
cross‐regulators
mammalian
transcripts
(like
cancer).
evidence
this
cross‐kingdom
gene
through
gathered
considerable
enthusiasm
scientific
field,
even
though
there
on‐going
debates
regarding
reproducibility
effectiveness
these
findings.
In
review,
we
provide
information
connect
medicinal
regulatory
properties
phytometabolites,
by
effects
on
regulating
downstream
metabolic
or
pathological
processes.
While
further
extensive
research
initiatives
good
clinical
required
prove
disapprove
findings,
understanding
regulations
will
important
implications
use
synthetic
artificial
effective
alternatives
providing
health
benefits.
Язык: Английский
Curcumin suppresses cell viability in breast cancer cell line by affecting the expression of miR-15a-5p
Turkish Journal of Biochemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 3, 2024
Abstract
Objectives
Curcumin
plays
a
leading
role
as
an
epigenetic
regulator
in
cancer.
miR-15a-5p
is
crucial
non-coding
RNA
for
breast
cancer
(BRCA)
and
various
cancers
due
to
its
tumor
suppressor
role.
In
our
study,
we
aimed
examine
the
curcumin/miR-15a-5p/target
gene
interaction
BRCA
cells.
Methods
The
effects
of
curcumin
on
cell
viability
MCF7
cells
were
examined
using
WST8
technique.
migration
was
determined
scratch
wound
assay.
level
detected
curcumin-treated
transfected
compared
control
groups
by
RT-qPCR.
Overexpressed
genes
found
bioinformatics
tools
(GSE41970
TCGA).
miR15a-5p
potential
target
miRNet
tool
selected
overlapped
between
GSE41970
TCGA.
Survival
analysis
GEPIA2
tool.
Relative
expression
levels
four
via
qPCR.
Results
Cell
scratch-wound
closure
rate
reduced
mimic
overexpressed
Eighty-three
dysregulated
upregulated
(in
Among
possible
tool,
10
overlapping
with
CCNE1
CHEK1
be
important
survival
BRCA.
BMI1
decreased
Conclusions
treatment
increased
downregulated
genes.
Curcumin/miR-15a-5p
may
much
stronger
negative
Язык: Английский