International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(23), С. 14744 - 14744
Опубликована: Ноя. 25, 2022
Inherited
metabolic
disorders
(IMD)
are
rare
medical
conditions
caused
by
genetic
defects
that
interfere
with
the
body's
metabolism.
The
clinical
phenotype
is
highly
variable
and
can
present
at
any
age,
although
it
more
often
manifests
in
childhood.
number
of
treatable
IMDs
has
increased
recent
years,
making
early
diagnosis
a
better
understanding
natural
history
disease
important
than
ever.
In
this
review,
we
discuss
main
challenges
faced
applying
proteomics
to
study
IMDs,
key
advances
achieved
field
using
tandem
mass
spectrometry
(MS/MS).
This
technology
enables
analysis
large
numbers
proteins
different
body
fluids
(serum,
plasma,
urine,
saliva,
tears)
single
each
sample,
even
be
applied
dried
samples.
MS/MS
thus
emerged
as
tool
choice
for
proteome
characterization
provided
new
insights
into
many
diseases
biological
systems.
last
10
sequential
window
acquisition
all
theoretical
fragmentation
spectra
(SWATH-MS)
an
accurate,
high-resolution
technique
identification
quantification
differentially
expressed
between
healthy
controls
IMD
patients.
Proteomics
particularly
promising
approach
help
obtain
information
on
diseases,
including
biomarkers
aid
underlying
pathophysiology
guide
development
therapies.
Here,
summarize
emerging
proteomic
technologies
current
uses
limitations
identify
quantify
proteins.
Moreover,
describe
use
mechanisms
regulating
complex
phenotypes;
area
research
essential
understand
these
other
human
diseases.
Journal of Proteome Research,
Год журнала:
2024,
Номер
23(2), С. 511 - 522
Опубликована: Янв. 3, 2024
Minimally
invasive
liquid
biopsies
from
the
eye
capture
locally
enriched
fluids
that
contain
thousands
of
proteins
highly
specialized
ocular
cell
types,
presenting
a
promising
alternative
to
solid
tissue
biopsies.
The
advantages
include
sampling
without
causing
irreversible
functional
damage,
potentially
better
reflecting
heterogeneity,
collecting
samples
in
an
outpatient
setting,
monitoring
therapeutic
response
with
sequential
sampling,
and
even
allowing
examination
disease
mechanisms
at
level
living
humans,
approach
we
refer
as
TEMPO
(Tracing
Expression
Multiple
Protein
Origins).
Liquid
biopsy
proteomics
has
potential
transform
molecular
diagnostics
prognostics
assess
personalized
strategies
individual
patients.
This
review
addresses
opportunities,
challenges,
future
directions
high-resolution
ophthalmology,
particular
emphasis
on
large-scale
collection
high-quality
samples,
cutting
edge
technology,
artificial
intelligence-supported
data
analysis.
Comprehensive Reviews in Food Science and Food Safety,
Год журнала:
2024,
Номер
23(2)
Опубликована: Март 1, 2024
Abstract
New
sources
of
proteins
are
essential
to
meet
the
demands
growing
world
population
and
evolving
food
trends.
Assessing
allergenicity
in
novel
(NF)
poses
a
significant
safety
regulatory
challenge.
The
Codex
Alimentarius
Commission
presented
an
assessment
protocol
for
genetically
modified
(GM)
foods,
which
can
also
be
adapted
NF.
Since
no
single
laboratory
test
adequately
predict
allergenic
potential
NF,
follows
weight‐of‐evidence
approach,
evaluated
by
experts,
as
part
risk
management
process.
Regulatory
bodies
worldwide
have
adopted
this
protocol,
which,
among
other
things,
promotes
global
harmonization.
This
review
unravels
reliability
various
motivations,
terms,
concepts,
approaches
assessments,
aiming
enhance
understanding
manufacturers
public.
Health
Canada,
Food
Safety
JAPAN,
Standards
Australia
Zealand
were
surveyed,
focusing
on
European
Authority
US
Administration
examples
scientific
opinions
regarding
assessments
GM
from
2019
2023.
According
our
findings,
current
NF
approval
primarily
rely
literature
reviews.
Only
few
proactively
additional
tests.
We
recommend
conducting
bioinformatic
analyses
when
panel
experts
deems
that
there
is
insufficient
prior
research.
Journal of Proteome Research,
Год журнала:
2024,
Номер
23(11), С. 4771 - 4788
Опубликована: Июль 22, 2024
Measuring
responses
in
the
proteome
to
various
perturbations
improves
our
understanding
of
biological
systems.
The
value
information
gained
from
such
studies
is
directly
proportional
number
proteins
measured.
To
overcome
technical
challenges
associated
with
highly
multiplexed
measurements,
we
developed
an
affinity
reagent-based
method
that
uses
aptamers
protein-like
side
chains
along
assay
takes
advantage
their
unique
properties.
As
hybrid
reagents,
modified
are
fully
comparable
antibodies
terms
binding
characteristics
toward
proteins,
including
epitope
size,
shape
complementarity,
and
specificity.
Our
combines
these
intrinsic
properties
serial
kinetic
proofreading
steps
allow
effective
partitioning
stable
specific
complexes
unstable
nonspecific
complexes.
use
orthogonal
methods
enhance
specificity
effectively
overcomes
severe
limitation
multiplexing
inherent
sandwich-based
methods.
currently
measures
half
encoded
human
genome
femtomolar
sensitivity,
broad
dynamic
range
exceptionally
high
reproducibility.
Using
machine
learning
identify
patterns
change,
have
tests
based
on
measurement
multiple
predictive
current
health
states
future
disease
risk
guide
a
holistic
approach
precision
medicine.
Frontiers in Neuroinformatics,
Год журнала:
2024,
Номер
18
Опубликована: Сен. 16, 2024
The
Religious
Order
Study
and
Memory
Aging
Project
(ROSMAP)
is
an
initiative
that
integrates
two
longitudinal
cohort
studies,
which
have
been
collecting
clinicopathological
molecular
data
since
the
early
1990s.
This
extensive
dataset
includes
a
wide
array
of
omic
data,
revealing
complex
interactions
between
levels
in
neurodegenerative
diseases
(ND)
aging.
Neurodegenerative
are
frequently
associated
with
morbidity
cognitive
decline
older
adults.
Omics
research,
conjunction
clinical
variables,
crucial
for
advancing
our
understanding
diagnosis
treatment
diseases.
summary
reviews
omics
research—encompassing
genomics,
transcriptomics,
proteomics,
metabolomics,
epigenomics,
multiomics—conducted
through
ROSMAP
study.
It
highlights
significant
advancements
mechanisms
underlying
diseases,
particular
focus
on
Alzheimer's
disease.
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(23), С. 14744 - 14744
Опубликована: Ноя. 25, 2022
Inherited
metabolic
disorders
(IMD)
are
rare
medical
conditions
caused
by
genetic
defects
that
interfere
with
the
body's
metabolism.
The
clinical
phenotype
is
highly
variable
and
can
present
at
any
age,
although
it
more
often
manifests
in
childhood.
number
of
treatable
IMDs
has
increased
recent
years,
making
early
diagnosis
a
better
understanding
natural
history
disease
important
than
ever.
In
this
review,
we
discuss
main
challenges
faced
applying
proteomics
to
study
IMDs,
key
advances
achieved
field
using
tandem
mass
spectrometry
(MS/MS).
This
technology
enables
analysis
large
numbers
proteins
different
body
fluids
(serum,
plasma,
urine,
saliva,
tears)
single
each
sample,
even
be
applied
dried
samples.
MS/MS
thus
emerged
as
tool
choice
for
proteome
characterization
provided
new
insights
into
many
diseases
biological
systems.
last
10
sequential
window
acquisition
all
theoretical
fragmentation
spectra
(SWATH-MS)
an
accurate,
high-resolution
technique
identification
quantification
differentially
expressed
between
healthy
controls
IMD
patients.
Proteomics
particularly
promising
approach
help
obtain
information
on
diseases,
including
biomarkers
aid
underlying
pathophysiology
guide
development
therapies.
Here,
summarize
emerging
proteomic
technologies
current
uses
limitations
identify
quantify
proteins.
Moreover,
describe
use
mechanisms
regulating
complex
phenotypes;
area
research
essential
understand
these
other
human
diseases.
