From gametes to zygote: Mechanistic advances and emerging possibilities in plant reproduction
PLANT PHYSIOLOGY,
Год журнала:
2024,
Номер
195(1), С. 4 - 35
Опубликована: Март 2, 2024
Язык: Английский
In vivo Haploid Induction via Parthenogenesis Gene ToPAR in Rice
Rice Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Perspectives of Genome Editing Mediated Haploid Inducer Systems in Legumes
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(3), С. 1154 - 1154
Опубликована: Янв. 29, 2025
Genome
editing-mediated
haploid
inducer
systems
(HISs)
present
a
promising
strategy
for
enhancing
breeding
efficiency
in
legume
crops,
which
are
vital
sustainable
agriculture
due
to
their
nutritional
benefits
and
ability
fix
nitrogen.
Traditional
is
often
slow
complicated
by
the
complexity
of
legumes’
genomes
challenges
associated
with
tissue
culture.
Recent
advancements
have
broadened
applicability
HISs
facilitating
reduction
duration
cycle.
By
integrating
genome
editing
technology
systems,
researchers
can
achieve
precise
genetic
modifications
rapidly
produce
homozygous
lines,
thereby
significantly
accelerating
development
desired
traits.
This
review
explores
current
status
future
prospects
legumes,
emphasizing
mechanisms
induction;
recent
breakthroughs;
existing
technical
challenges.
Furthermore,
we
highlight
necessity
additional
research
optimize
these
across
various
species,
has
potential
greatly
enhance
contribute
sustainability
production.
Язык: Английский
Widespread application of apomixis in agriculture requires further study of natural apomicts
iScience,
Год журнала:
2024,
Номер
27(9), С. 110720 - 110720
Опубликована: Авг. 14, 2024
Apomixis,
or
asexual
reproduction
through
seeds,
is
frequent
in
nature
but
does
not
exist
any
major
crop
species,
yet
the
phenomenon
has
captivated
researchers
for
decades
given
its
potential
clonal
seed
production
and
plant
breeding.
A
discussion
on
whether
this
field
will
benefit
from
continued
study
of
natural
apomicts
warranted
recent
outstanding
progress
engineering
apomixis.
Here,
we
summarize
what
known
about
genetic
control
status
applying
synthetic
apomixis
agriculture.
We
argue
there
still
much
to
be
learned
apomicts,
learning
them
necessary
improve
current
guarantee
effective
application
beyond
few
genera
it
shown
promise
so
far.
Specifically,
stress
value
studying
repeated
evolution
a
phylogenetic
comparative
-omics
context.
Finally,
identify
questions
discuss
how
technological
advancements
can
used
help
close
these
knowledge
gaps.
In
particular,
genomic
resources
are
lacking
must
remedied
widespread
use
Язык: Английский
Self-propagated clonal seed production in dicotyledonous Arabidopsis
Science Bulletin,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 1, 2024
Язык: Английский
Meiosis requires m6A modification for selection of targets in plants
New Phytologist,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 2, 2024
'These
observations
provide
evidence
that
posttranscriptional
m6A
modification
is
required
for
maintaining
the
activity
of
meiotic
genes,
and
stage-specific
deposition
removal
ensure
orderly
progression
meiosis.'
Meiosis,
a
specialized
form
cell
division,
halves
diploid
chromosome
numbers
to
generate
haploid
gametophytes,
which
essential
sexual
reproduction
in
most
eukaryotes.
Meiotic
recombination
not
only
facilitates
exchange
genetic
information
between
homologous
chromosomes
(homologs),
but
also
assures
their
subsequent
proper
segregation,
has
great
impact
on
diversity
genomic
integrity
progenies.
initiated
from
programmed
formation
DSBs,
catalyzed
by
evolutionarily
conserved
type-II
DNA
topoisomerase
SPO11-1
MTOPVIB
complex.
The
repair
DSBs
can
result
homologs
known
as
crossovers
(COs)
noncrossovers
(NCOs)
(Wang
&
Copenhaver,
2018;
Zickler
Kleckner,
2023).
In
past
30
years,
molecular
studies
have
identified
dozens
genes
involved
regulating
plants,
including
some
epigenetic
factors
2018).
However,
function
RNA
related
this
process
largely
unknown.
Xue
et
al.
made
first
step
towards
understanding
mechanism
eraser
ALKBH5
DSB
rice
meiosis.
They
started
project
identifying
male
sterile
mutant
gamma-irradiated
library.
author
further
cloned
target
OsALKBH5
gene
demonstrated
its
mutations
affected
during
meiosis
I,
thus
leading
sterility.
preferentially
localizes
cytoplasm
nucleoplasm
pollen
mother
cells
(PMCs),
microspores,
tapetal
cells.
confirmed
demethylase
vitro
vivo,
mutation
impacts
modifications
stability
mRNA
Further
showed
Osalkbh5
displays
upmethylated
associated
downregulated
such
PAIR2,
PAIR3,
OsCOM1,
OsZIP4,
HEIP1,
are
recombination.
These
results
suggest
participates
mediating
targeted
maintain
(Fig.
1).
Similarly,
deficiency
mice
Alkbh5
homolog
resulted
increased
infertility
spermatocytes
(Zheng
al.,
2013).
study
ALKBH5-mediated
erasure
mRNAs
correct
splicing
selective
degradation
long
3′UTR
transcripts
spermatids
(Tang
Therefore,
may
serve
fundamental
basis
shared
plants
animals,
while
appear
be
diversified
across
different
species
2013;
Tang
As
expected,
very
recent
revealed
comprehensive
maps
at
single-base
precision
tissues
throughout
life
cycle
both
Arabidopsis.
Comparative
analysis
with
mammals
uncovered
existence
comparable
distribution
patterns
sites
2024).
It
been
observed
expression
numerous
occurs
before
initiation
maize
meiosis,
certain
exhibit
varying
levels
transcript
abundance
stages
(Nelms
Walbot,
2019).
mammals,
'writer'
Mettl3
'eraser'
reported
regulate
(Xu
2017;
By
contrast,
regulates
spermatogonia
differentiation
initiation,
whereas
functions
spermatogenic
round
spermatids.
Consistently,
inactivation
METTL3
causes
much
earlier
arrest
than
Alkbh5-knockout
Xu
2017).
ensures
This
conclusion
supported
finding
multiple
meiosis-specific
regulated
OsALKBH5-induced
erasure.
identification
responsible
well
relationship
'eraser',
special
targets,
needs
investigated.
al.'s
raises
other
interesting
questions.
For
example,
expressed
meiocytes
tapetum,
indicating
potential
role
either
tapetum
or
development.
pointed
out
that,
large
number
unexpected
decreased
methylation
mutant,
OsMTOPVIB
formation.
presents
question:
how
does
knockout
reduce
transcripts.
Recent
closely
correlated
factors,
methylation,
histone
modifications,
microRNAs,
noncoding
RNAs,
chromatin
remodeling
(Hu
2024),
more
complex
manner.
A
tomatoes
SlALKBH2
SlDML2
feedback
loop
fruit
ripening
(Zhou
mammalian
cells,
H3K36me3
recognized
bound
directly
writer
complex,
subsequently
mark
onto
actively
transcribed
RNAs
(Huang
bidirectional
interactions
regulators
commonly
exist
among
deserves
exploration
Funding
provided
Guangdong
Laboratory
Lingnan
Modern
Agriculture
(NG2022002),
National
Natural
Science
Foundation
China
(Grant
Nos.
31925005
32000246),
Double
first-class
discipline
promotion
SCAU
(2023B10564004).
Язык: Английский
Microspore embryogenesis: in vitro cultivation induced cell reprogramming for plant breeding
Seed Biology,
Год журнала:
2020,
Номер
0(0), С. 1 - 11
Опубликована: Янв. 1, 2020
Язык: Английский
Loss of function of <i>CENH3</i> causes genome instability in soybean
Seed Biology,
Год журнала:
2023,
Номер
2(1), С. 0 - 0
Опубликована: Янв. 1, 2023
Centromeres
are
the
site
where
kinetochores
assemble
and
spindle
microtubules
anchor
to
chromosome
during
cell
division.
epigenetically
specified
by
centromeric
histone
H3
(CENH3).
The
imbalance
of
CENH3
loading
rate
or
dosage
on
parental
centromeres
often
leads
uniparental
elimination
in
offspring.
A
body
studies
genome
stability
have
been
reported
Arabidopsis,
cotton,
many
other
monocots,
but
not
soybean
(Glycine
max),
an
important
dicot
crop.
In
our
study,
we
identified
a
single-copy
functional
CENH3
found
its
role
parent-of-origin
effect
caused
mutation
conserved
glycine
genetic
background.
This
study
provide
evidence
that
knockout
has
potential
induce
would
shed
light
future
development
CENH3-based
haploid
induction
(HI)
system
centromere
biology
soybean.
Язык: Английский