Improving sulforaphane content in broccoli sprouts by applying Se: transcriptome profiling and coexpression network analysis provide insights into the mechanistic response DOI Open Access

Shuxiang Mao,

Junwei Wang, Zhijun Guo

и другие.

Physiologia Plantarum, Год журнала: 2025, Номер 177(1)

Опубликована: Янв. 1, 2025

Abstract Sulforaphane (SF) is a sulfur (S)‐containing isothiocyanate found in cruciferous vegetables and known for its potent anticancer properties. Broccoli sprouts, particular, are considered safe healthy dietary choices due to their high SF content other beneficial biological activities, such as enhanced metabolite ingestion. The application of selenium (Se) an excellent approach enhance the abundance SF. Previous studies have often focused on gene expression changes synthetic substrates glucoraphanin (RAA) explain variation response Se application. However, regulatory network physiological biochemical reactions involved regulation biosynthesis poorly understood. In this study, Se‐treated broccoli sprouts had higher RAA contents; they increased with increasing Using RNA‐seq combination KEGG, GO, phenotypic, WGCNA analyses, it was observed that not only induced but also glutathione serves S donor acts oxidative stress reliever result treatment. Additionally, module related glucosinolate identified. Yeast one‐hybrid system dual luciferase reporter assay were utilized. These assays demonstrated hub transcription factors GATA22, ERF12‐like, MYB108 would directly bind SUR1 promoter positively regulate expression. Our study presents first global overview role GSH metabolism biosynthesis, provides novel valuable resource molecular breeding high‐SF broccoli.

Язык: Английский

Comprehensive overview of glucosinolates in crucifers: occurrence, roles, metabolism, and transport mechanisms—a review DOI
Sanjula Sharma, Heena Rani, Gurpreet Kaur

и другие.

Phytochemistry Reviews, Год журнала: 2024, Номер unknown

Опубликована: Сен. 26, 2024

Язык: Английский

Процитировано

6
Improving sulforaphane content in broccoli sprouts by applying Se: transcriptome profiling and coexpression network analysis provide insights into the mechanistic response DOI Open Access

Shuxiang Mao,

Junwei Wang, Zhijun Guo

и другие.

Physiologia Plantarum, Год журнала: 2025, Номер 177(1)

Опубликована: Янв. 1, 2025

Abstract Sulforaphane (SF) is a sulfur (S)‐containing isothiocyanate found in cruciferous vegetables and known for its potent anticancer properties. Broccoli sprouts, particular, are considered safe healthy dietary choices due to their high SF content other beneficial biological activities, such as enhanced metabolite ingestion. The application of selenium (Se) an excellent approach enhance the abundance SF. Previous studies have often focused on gene expression changes synthetic substrates glucoraphanin (RAA) explain variation response Se application. However, regulatory network physiological biochemical reactions involved regulation biosynthesis poorly understood. In this study, Se‐treated broccoli sprouts had higher RAA contents; they increased with increasing Using RNA‐seq combination KEGG, GO, phenotypic, WGCNA analyses, it was observed that not only induced but also glutathione serves S donor acts oxidative stress reliever result treatment. Additionally, module related glucosinolate identified. Yeast one‐hybrid system dual luciferase reporter assay were utilized. These assays demonstrated hub transcription factors GATA22, ERF12‐like, MYB108 would directly bind SUR1 promoter positively regulate expression. Our study presents first global overview role GSH metabolism biosynthesis, provides novel valuable resource molecular breeding high‐SF broccoli.

Язык: Английский

Процитировано

0