Comment on bg-2021-25 DOI Creative Commons

Опубликована: Март 14, 2021

Abstract. Marine sedimentation rate and bottom-water O2 concentration control organic carbon remineralization sequestration across continental margins, but whether how they shape microbiome architecture (the ultimate effector of all biogeochemical phenomena) shelf slope sediments is still unclear. Here we reveal distinct structures functions, amidst comparable pore fluid chemistries, along 300 cm sediment horizons underlying the seasonal (shallow coastal; water depth: 31 m) perennial (deep sea; depths: 530 580 m) oxygen minimum zones (OMZs) Arabian Sea, situated western Indian margin. The sedimentary geomicrobiology was elucidated by analyzing metagenomes, metatranscriptomes, enrichment cultures, depositional rates measured via radiocarbon lead excess dating; findings were then evaluated in light other geochemical data available for cores. Along perennial-OMZ cores, microbial communities dominated Gammaproteobacteria Alphaproteobacteria, seasonal-OMZ core Euryarchaeota Firmicutes. As a signature, cryptic methane production–consumption cycle found to operate near surface, within sulfate reduction zone; overall diversity, as well relative abundances anaerobes requiring simple fatty acids (methanogens, anaerobic oxidizers, reducers, acetogens), peaked topmost layer declined synchronized fluctuations until sulfate–methane transition zone reached. profile completely reversed horizon. In sediments, deposited higher rich marine components that degrade readily acids; simultaneously, lower afforded exposure time matter degradation despite hypoxia bottom water. resultant abundance reduced substrates eventually sustained multiple inter-competing processes upper layers. entire geomicrobial scenario opposite OMZ. These create microbiological baseline understanding carbon–sulfur cycling depositional settings column oxygenation regimes margins.

Язык: Английский

Microbial Diversity and Sulfur Cycling in an Early Earth Analogue: From Ancient Novelty to Modern Commonality DOI
C. Ryan Hahn, Ibrahim Farag, Chelsea L. Murphy

и другие.

mBio, Год журнала: 2022, Номер 13(2)

Опубликована: Март 8, 2022

Life emerged and diversified in the absence of molecular oxygen. The prevailing anoxia unique sulfur chemistry Paleo-, Meso-, Neoarchean early Proterozoic eras may have supported microbial communities that differ from those currently thriving on earth's surface. Zodletone spring southwestern Oklahoma represents a habitat where spatial sampling could substitute for geological namely, anoxic, surficial light-exposed sediments simulating preoxygenated earth to overlaid water column air exposure simulates oxygen intrusion during Neoproterozoic era. We document remarkably diverse community anoxic sediments, with 340/516 (65.89%) genomes recovered metagenomic survey belonging 200 bacterial archaeal families were either previously undescribed or exhibit an extremely rare distribution current earth. Such diversity is underpinned by widespread occurrence sulfite, thiosulfate, tetrathionate, reduction paucity sulfate machineries these taxa. Hence, processes greatly expand lineages mediating reductive sulfur-cycling tree life. An analysis overlaying oxygenated demonstrated development significantly less dominated well-characterized prevalence oxidative processes. transition ancient novelty modern commonality underscores profound impact great oxygenation event community. It also suggests novel encountered anaerobic habitats represent taxa once thrived but failed adapt progressive oxygenation.

Язык: Английский

Процитировано

28
Identification of sulfur species in coastal marine sediments collected from the Seto Inland Sea, Japan in summer DOI
Satoshi Asaoka, Kazuhiko Takeda, Misaki Katayama

и другие.

Estuarine Coastal and Shelf Science, Год журнала: 2025, Номер 313, С. 109130 - 109130

Опубликована: Янв. 10, 2025

Язык: Английский

Процитировано

0
Aerobic microbial communities in the sediments of a marine oxygen minimum zone DOI Creative Commons
Sabyasachi Bhattacharya, Chayan Roy,

Subhrangshu Mandal

и другие.

FEMS Microbiology Letters, Год журнала: 2020, Номер 367(19)

Опубликована: Сен. 23, 2020

ABSTRACT The ecology of aerobic microorganisms is never explored in marine oxygen minimum zone (OMZ) sediments. Here we reveal bacterial communities along ∼3 m sediment-horizons the eastern Arabian Sea OMZ. Sulfide-containing sediment-cores retrieved from 530 mbsl (meters beneath sea-level) and 580 were at 15–30 cm intervals, using metagenomics, pure-culture-isolation, genomics metatranscriptomics. Genes for respiration, oxidation methane/ammonia/alcohols/thiosulfate/sulfite/organosulfur-compounds, detected metagenomes all 25 sediment-samples explored. Most probable numbers chemolithoautotrophs chemoorganoheterotrophs individual sample-sites up to 1.1 × 107 (g sediment)-1. sediment-sample collected 275 cmbsf (centimeters seafloor) 530-mbsl-core yielded many such obligately isolates belonging Cereibacter, Guyparkeria, Halomonas, Methylophaga, Pseudomonas Sulfitobacter which died upon anaerobic incubation, despite being provided with possible electron acceptors fermentative substrates. High percentages metatranscriptomic reads sediment-sample, metagenomic sediment-samples, matched isolates’ genomic sequences including those metabolisms, genetic/environmental information processing cell division, thereby illustrating bacteria's in-situ activity, ubiquity across sediment-horizons, respectively. findings hold critical implications organic carbon sequestration/remineralization, inorganic compounds oxidation, within sediment realm global OMZs.

Язык: Английский

Процитировано

25
Sulfated and Sulfur-Containing Steroids and Their Pharmacological Profile DOI Creative Commons

Tatyana A. Pounina,

Tatyana A. Gloriozova,

Nick Savidov

и другие.

Marine Drugs, Год журнала: 2021, Номер 19(5), С. 240 - 240

Опубликована: Апрель 24, 2021

The review focuses on sulfated steroids that have been isolated from seaweeds, marine sponges, soft corals, ascidians, starfish, and other invertebrates. Sulfur-containing triterpenoids are sourced sedentary coelenterates, plants, sediments, crude oil, geological deposits. presents the pharmacological profile of steroids, sulfur-containing triterpenoids, which is based data obtained using PASS program. In addition, several semi-synthetic synthetic epithio represent a rare group bioactive lipids not yet found in nature, but possess high level antitumor activity, were included this for comparative characterization class compounds. About 140 presented review, demonstrate wide range biological activities. Therefore, out 71 thirteen show strong activity with confidence more than 90%, 50 only four 93%, eighteen 91% to 97.4%.

Язык: Английский

Процитировано

22
Heterotrophic Sulfur Oxidation of Halomonas titanicae SOB56 and Its Habitat Adaptation to the Hydrothermal Environment DOI Creative Commons
Rui Du, Di Gao, Yi‐Ting Wang

и другие.

Frontiers in Microbiology, Год журнала: 2022, Номер 13

Опубликована: Июнь 14, 2022

Halomonas bacteria are ubiquitous in global marine environments, however, their sulfur-oxidizing abilities and survival adaptations hydrothermal environments not well understood. In this study, we characterized the sulfur oxidation ability metabolic mechanisms of titanicae SOB56, which was isolated from sediment Tangyin field Southern Okinawa Trough. Physiological characterizations showed that it is a heterotrophic bacterium can oxidize thiosulfate to tetrathionate, with Na2S2O3 degradation reaching 94.86%. Two potential dehydrogenase-related genes, tsdA tsdB, were identified as encoding key catalytic enzymes, expression levels strain SOB56 significantly upregulated. Nine fifteen examined genomes possess TsdA- TsdB-homologous proteins, whose amino acid sequences have two typical Cys-X2-Cys-His heme-binding regions. Moreover, process H. might be regulated by quorum sensing, autoinducer-2 synthesis protein LuxS its genome. Regarding underlying adaptation environment, capable forming biofilms producing EPS. addition, genes related complete flagellum assembly system, various signal transduction histidine kinases, heavy metal transporters, anaerobic respiration, variable osmotic stress regulation also identified. Our results shed light on functions cycle revealed possible for living at deep-sea fields SOB56.

Язык: Английский

Процитировано

10
Deep-Sea In Situ Insights into the Formation of Zero-Valent Sulfur Driven by a Bacterial Thiosulfate Oxidation Pathway DOI Creative Commons

Ruining Cai,

Wanying He,

Rui Liu

и другие.

mBio, Год журнала: 2022, Номер 13(4)

Опубликована: Июль 19, 2022

Zero-valent sulfur (ZVS) distributes widely in the deep-sea cold seep, which is an important immediate cycle of seep. In our previous work, we described a novel thiosulfate oxidation pathway determined by dehydrogenase (TsdA) and thiosulfohydrolase (SoxB) mediating conversion to ZVS seep bacterium Erythrobacter flavus 21-3. However, occurrence ecological role this were obscure. Here, cultured E. 21-3 for 10 days demonstrated its capability forming situ field. Based on proteomic, stoichiometric analyses microscopic observation, found that benefited adapt conditions. Notably, ~25% metagenomes assembled genomes derived from shallow sediments seeps contained both tsdA soxB, where presented abundant metabolism-related genes active cycle. Our results suggested TsdA SoxB existed across many bacteria inhabiting frequently used microbes take part biogeochemical IMPORTANCE The contribution has received considerable attention recent years. study, isolated laboratorial condition. It provided new clue about formation because huge differences between laboratory environment, whether perform same should be further confirmed. verified formed using through cultivation, confirmed importance approach study real metabolism microorganisms.

Язык: Английский

Процитировано

9
Sedimentation rate and organic matter dynamics shape microbiomes across a continental margin DOI Creative Commons
Sabyasachi Bhattacharya, Tarunendu Mapder,

Svetlana Fernandes

и другие.

Biogeosciences, Год журнала: 2021, Номер 18(18), С. 5203 - 5222

Опубликована: Сен. 23, 2021

Abstract. Marine sedimentation rate and bottom-water O2 concentration control organic carbon remineralization sequestration across continental margins, but whether how they shape microbiome architecture (the ultimate effector of all biogeochemical phenomena) shelf slope sediments is still unclear. Here we reveal distinct structures functions, amidst comparable pore fluid chemistries, along 300 cm sediment horizons underlying the seasonal (shallow coastal; water depth: 31 m) perennial (deep sea; depths: 530 580 oxygen minimum zones (OMZs) Arabian Sea, situated western Indian margin. The sedimentary geomicrobiology was elucidated by analyzing metagenomes, metatranscriptomes, enrichment cultures, depositional rates measured via radiocarbon lead excess dating; findings were then evaluated in light other geochemical data available for cores. Along perennial-OMZ cores, microbial communities dominated Gammaproteobacteria Alphaproteobacteria, seasonal-OMZ core Euryarchaeota Firmicutes. As a signature, cryptic methane production–consumption cycle found to operate near surface, within sulfate reduction zone; overall diversity, as well relative abundances anaerobes requiring simple fatty acids (methanogens, anaerobic oxidizers, reducers, acetogens), peaked topmost layer declined synchronized fluctuations until sulfate–methane transition zone reached. profile completely reversed horizon. In sediments, deposited higher rich marine components that degrade readily acids; simultaneously, lower afforded exposure time matter degradation despite hypoxia bottom water. resultant abundance reduced substrates eventually sustained multiple inter-competing processes upper layers. entire geomicrobial scenario opposite OMZ. These create microbiological baseline understanding carbon–sulfur cycling settings column oxygenation regimes margins.

Язык: Английский

Процитировано

11
34S enrichment as a signature of thiosulfate oxidation in the “Proteobacteria” DOI
Masrure Alam,

Svetlana Fernandes,

Subhrangshu Mandal

и другие.

FEMS Microbiology Letters, Год журнала: 2021, Номер 368(12)

Опубликована: Июнь 1, 2021

ABSTRACT Kinetics of thiosulfate oxidation, product and intermediate formation, 34S fractionation, were studied for the members Alphaproteobacteria Paracoccus sp. SMMA5 Mesorhizobium thiogangeticum SJTT, Betaproteobacteria member Pusillimonas ginsengisoli SBO3, Acidithiobacillia Thermithiobacillus SMMA2, during chemolithoautotrophic growth in minimal salts media supplemented with 20 mM thiosulfate. The two oxidized directly to sulfate, progressively enriching end-product 34S; Δ34Sthiosulfate-sulfate values recorded at end processes (when no was any further) −2.9‰ −3.5‰, respectively. SBO3 on other hand, sulfate via tetrathionate progressive enrichment throughout incubation period; Δ34Sthiosulfate-sulfate, further oxidation took place), reached −3.5‰ −3.8‰, Based similar fractionation patterns previously by strains pantotrophus, Advenella kashmirensis Hydrogenovibrio crunogenus, it concluded that reverse 34S, could be a characteristic signature bacterial oxidation.

Язык: Английский

Процитировано

7
Spatial and temporal distribution of hydrogen sulfide and sulfur species in coastal marine sediments collected from Osaka Bay, Japan DOI
Satoshi Asaoka, T. Endo,

Yasuhiro Ushihara

и другие.

Marine Chemistry, Год журнала: 2023, Номер 251, С. 104233 - 104233

Опубликована: Март 11, 2023

Язык: Английский

Процитировано

2
Biogeochemistry of Marine Oxygen Minimum Zones with Special Emphasis on the Northern Indian Ocean DOI

Svetlana Fernandes,

Subhrangshu Mandal,

Kalyani Sivan

и другие.

Опубликована: Апрель 1, 2022

Oxygen minimum zones (OMZs) enclose O 2 depleted subsurface water masses in the global ocean extending approximately 150 to 1200 m below sea level. The most pronounced OMZs occur Eastern Tropical North Pacific off Mexico and California (ETNP), South Peru Chile (ETSP), Arabian Sea (AS) defined by secondary nitrite maxima attributed intense denitrification column. These sites are critical for biogeochemical processes that control biodiversity primary productivity of ocean. preservation organic carbon is efficient within sediments underlying oxygen-depleted waters as a result incomplete decomposition it sinks through column diminished bioturbation activity. partially degraded (reactive) matter fuels microbe-mediated anoxic marine where sulfate reduction significant remineralization pathway. exert strong influence on abundance, diversity, composition microbial communities. Recent geochemical environmental genomic studies identified prevalence C, N, S cycles OMZs. Here, we review progress current understanding C–S–N cycle OMZ with regard its biogeochemistry ecology, present brief account mechanism formation northern Indian Ocean.

Язык: Английский

Процитировано

3