Overwintering Fish Community in Ice‐Covered Environments in Hokkaido, Japan, Inferred From Environmental DNA
Environmental DNA,
Год журнала:
2025,
Номер
7(2)
Опубликована: Март 1, 2025
ABSTRACT
The
overwintering
ecology
of
fish
in
the
seasonal
ice
zones
(SIZs)
remains
largely
unexplored
owing
to
methodological
limitations.
Environmental
DNA
(eDNA)
can
reveal
distribution
and
diversity
species
various
aquatic
environments,
thereby
offering
a
possible
solution
limitations
SIZ
studies.
Therefore,
we
aimed
detect
community
ice‐covered
Saroma‐ko
Lagoon,
located
on
Okhotsk
Sea
coast
Hokkaido,
its
inflow,
using
eDNA
metabarcoding.
extracted
from
under‐ice
seawater
collected
lagoon
yielded
28
taxa,
predominantly
Clupea
pallasii
based
relative
read
abundance.
Dissimilarity
analysis
suggested
short‐term
temporal
variations
composition
seawater,
even
at
same
site.
Nineteen
Tribolodon
brandtii
T.
hakonensis
,
were
detected
river
water.
high
dissimilarity
between
results
water
segregation
river.
Fish
meltwater
sea
was
assigned
five
suggesting
entrainment
particulate
matter
containing
during
growth.
true
richness
estimated
discrepancies
with
historical
reports
suggest
that
sampling
efforts
need
be
optimized
for
environments
promote
more
comprehensive
detection.
This
study
demonstrated
usefulness
metabarcoding
under
ice.
Clarifying
shedding
patterns,
persistence,
dispersal
would
improve
reliability
this
technique
expand
use
SIZs.
Язык: Английский
A novel probe to sample dissolved and particulate matter in sea ice at high vertical resolution
Elementa Science of the Anthropocene,
Год журнала:
2025,
Номер
13(1)
Опубликована: Янв. 1, 2025
Sea
ice
can
be
interspersed
vertically
with
both
permeable
and
impermeable
layers.
These
features
smaller
than
a
few
centimetres
but
are
important
for
understanding
biogeochemical
cycles
in
sea
ice.
Traditionally,
sea-ice
samples
collected
by
drilling
into
the
rotating
core
barrel
cutting
head.
Ice
cores
extracted
then
cut
sections
that
melted
to
collect
variable
of
interest.
Drawbacks
this
method
include
difficulty
thick
contamination
samples.
Brines
may
also
drain
from
their
in-situ
locations
lost,
meaning
micro-environments
overlooked
or
misrepresented.
To
address
these
drawbacks,
we
developed
melt
probe
bores
collects
high-resolution
The
capability
delineate
layer
dye
artificial
was
tested
during
cold
laboratory-based
experiments.
Complementary
field
experiments
were
carried
out
first-year
landfast
February–March
2023
at
Saroma-ko
Lagoon,
Japan.
Experiments
consisted
testing
high-vertical-resolution
as
well
its
ability
deployed
on
snow-covered
able
finer
resolution
(20
mm)
traditional
coring
(approximately
50–100
could
sample
brine
high
vertical
resolution.
Melt-probe
used
measure
iron
without
contamination.
This
proof-of-concept
study
provides
an
alternative
sampling
support
new
research
fine-scale
structures,
applications
dissolved
particulate
constituents
including
trace
metals,
macro-nutrients,
microplastics
gases.
Язык: Английский