Cas9-expressing cattle using all-in-one CRISPR/Cas9 for bovine genome editing DOI Creative Commons
Dong-Hyeok Kwon, Gyeong‐Min Gim, Soo‐Young Yum

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 12, 2024

Abstract Background Livestock, particularly cattle, are crucial for biotechnology fields, such as genetic breeding, infectious diseases, bioreactors, and specific disease models. However, engineering in cattle has lagged due to long gestation periods, single embryo pregnancies, high rearing costs. Additionally, the slow validation of germline transmission absence germline-competent embryonic stem cells hinder progress. With development genome editing technologies like ZFN, TALEN, CRISPR-Cas9, recent advancements have shown that Cas9-expressing pigs chickens been successfully produced. We hypothesize generating CRISPR/Cas9-expressing their resources will provide a powerful resource bovine editing, advancing our understanding genetics resistance. Results In this study, two types were produced: Cas9-RFP-FatI, Cas9-GFP-sgPRNP. Somatic from these induced mutate several target genes when single-guide RNAs (sgRNAs) transfected into somatic cells. semen CRISPR/Cas9 expressing male was frozen used fertilize wild-type oocytes, transmitting transgene (Cas9, reporter genes, fatty acid dehydrogenase I [FatI]), sgRNA prion protein [PRNP]) next generation. Furthermore, gene capabilities Cas9, including knockout high-efficiency knock-in, confirmed embryos derived F1 through vitro production. Conclusion These data demonstrate, first time, born, transmitted next-generation calves (F1) F2 embryos. addition, germ F0 F1generations evaluate potential (knockout knock-in) multiple genes. PRNP-mutated currently being raised resistance model spongiform encephalopathy. transgenic models derivatives serve valuable both vivo genomics diseases.

Язык: Английский

Non-destructive pre-incubation sex determination in chicken eggs using hyperspectral imaging and machine learning DOI Creative Commons
Md Wadud Ahmed,

Asher Sprigler,

J.L. Emmert

и другие.

Food Control, Год журнала: 2025, Номер unknown, С. 111233 - 111233

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Cas9-expressing cattle using the PiggyBac transposon all-in-one system DOI Creative Commons
Dong-Hyeok Kwon, Gyeong‐Min Gim, Soo‐Young Yum

и другие.

BMC Genomics, Год журнала: 2025, Номер 26(1)

Опубликована: Март 5, 2025

Livestock, particularly cattle, are crucial for biotechnology fields, such as genetic breeding, infectious diseases, bioreactors, and specific disease models. However, engineering in cattle has lagged due to long gestation periods, single embryo pregnancies, high rearing costs. Additionally, the slow validation of germline transmission absence germline-competent embryonic stem cells hinder progress. With development genome editing technologies like ZFN, TALEN, CRISPR-Cas9, recent advancements have shown that Cas9-expressing pigs chickens been successfully produced. We hypothesize generating CRISPR/Cas9-expressing their resources will provide a powerful resource bovine editing, advancing our understanding genetics resistance. In this study, two types were produced: Cas9-RFP-fatty acid dehydrogenase I (FatI), Cas9-GFP-sgRNA prion protein (sgPRNP). Somatic from these induced mutate multiple target genes when single-guide RNAs (sgRNAs) transfected into somatic cells. semen Cas9 expressing male was frozen used fertilize wild-type oocytes, transmitting transgene (Cas9, reporter genes, FatI), sgPRNP) next generation. Furthermore, gene capabilities Cas9, including knockout high-efficiency knock-in, confirmed embryos derived F1 through vitro production. These data demonstrate, first time, born, transmitted next-generation calves (F1) F2 embryos. addition, germ F0 F1generations evaluate potential (knockout knock-in) genes. PRNP-mutated currently being raised resistance model spongiform encephalopathy. transgenic models derivatives serve valuable both vivo genomics diseases.

Язык: Английский

Процитировано

0
Editing the CYP19 Gene in Goat Embryos Using CRISPR/Cas9 and Somatic Cell Nuclear Transfer Techniques DOI

Ahmad Pirali,

Farnoosh Jafarpour, Mehdi Hajian

и другие.

Cellular Reprogramming, Год журнала: 2025, Номер unknown

Опубликована: Март 24, 2025

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) system is revolutionizing genome engineering and expected to bring significant advancements in livestock traits, including the treatment of genetic diseases. This study focuses on CRISPR/Cas9-mediated modifications CYP19 gene, which encodes aromatase, an enzyme crucial for converting testosterone estrogen essential steroid metabolism. Guide RNAs (gRNAs) were designed target gene cloned into pX459 vector. recombinant plasmid was then electrotransfected fibroblast cells from a Lori-Bakhtiari buck, these transfected used embryo production via somatic cell nuclear transfer (SCNT). embryos evaluated their progression through embryonic stages, showing no difference blastocyst development between knock-out unedited groups. knockout efficiency 78.4% 68.9% goat blastocysts, demonstrating successful depletion CYP19. We successfully achieved high rate gene-edited combined application electrotransfection SCNT technologies, while maintaining normal developmental embryos. These can be generate goats, providing foundation further studies CYP19's role male fertility traits.

Язык: Английский

Процитировано

0
A Systematic Review of Opportunities and Limitations of Innovative Practices in Sustainable Agriculture DOI Creative Commons
Anita Boros,

Eszter Szólik,

Goshu Desalegn

и другие.

Agronomy, Год журнала: 2024, Номер 15(1), С. 76 - 76

Опубликована: Дек. 30, 2024

A transformation is needed in agricultural production to satisfy the growing population-based increased food demands and overcome safety challenges while supporting human health preservation of natural resources. Improving sustainable practices essential for developing a long-term resilient economy. This systematic review with meta-analyses aims overview agriculture’s progress, trends, future opportunities, focusing primarily on integrating novel technologies into recently prevailing operations. As basis, an extensive literature search was conducted, identifying nearly 45,000 records related closely various dimensions agriculture. Out these, based thorough selection process, 291 were included further evaluations. The temporal distribution content analyses indicated improvement number papers spectrum addressed aspects. analysis publications revealed that most traditional farming focus mass cannot deal interrelated sustainability challenges. On other hand, consider sustainability’s environmental, economic, social aspects, thereby preserving environmental economic viability well-being. integrative assessment this study suggests adopting novel, innovative can achieve significant progress toward sustainability. At same time, broad range limitations are listed, which widely hinder penetration initiatives. To eliminate these barriers, consistent policy regulations required, targeting specific problems, alongside complex, education-based support system, boosting initiatives green transition

Язык: Английский

Процитировано

2
Advancement of animal and poultry nutrition: Harnessing the power of CRISPR-Cas genome editing technology DOI Creative Commons
Mahbuba Akther Mishu, Sabuj Kanti Nath, M. Sohidullah

и другие.

Journal of Advanced Veterinary and Animal Research, Год журнала: 2024, Номер 11(2), С. 483 - 483

Опубликована: Янв. 1, 2024

CRISPR-associated proteins and clustered regularly interspaced short palindromic repeats (CRISPR-Cas) technology has emerged as a groundbreaking advancement in animal poultry nutrition to improve feed conversion efficiency, enhance disease resistance, the nutritional quality of products. Despite significant advancements, there is research gap systematic understanding comprehensive use CRISPR-Cas method nutrition. The purpose this study elucidate latest advancements through genome editing technology, focusing on gene manipulation metabolism, immunity, growth. Following preferred reporting items meta-analysis reviews guidelines, we conducted search using several databases, including Scopus, PubMed, Web Science, until May 2024, finally, included total 108 articles study. This article explores system additives like probiotics enzymes, which could reduce antibiotics production. Furthermore, discusses ethical regulatory issues related nutrition, concerns about welfare, food safety, environmental impacts. Overall, holds substantial promise overcome challenges modern agriculture. By enriching products, increasing improving it offers sustainable cost-effective solutions that can revolutionize

Язык: Английский

Процитировано

1
Futures of Livestock Industry from the Viewpoints of Jurisprudence and Regulatory Measures in the Philippines DOI Creative Commons

Arnolfo M. Monleon

IntechOpen eBooks, Год журнала: 2024, Номер unknown

Опубликована: Июль 23, 2024

The livestock industry, comprising the sources of protein foods (eggs, meat, and milk), contributing enormous income, balancing trade, attaining food requirements in Philippines. Filipinos have unique preferences, traditional delicacies are served usually important events showcasing rich cultures gastronomic traditions. Thereby, consumption demands for animal products will continue to rise associated with increasing trends human population economic status. sustainability progress however, challenged continuously by disruptions production input supply disease outbreaks, natural calamities, regulatory measures on biosecurity, importation, urbanization. This review provides outlooks viewpoints considering issues issuances affecting directly or indirectly future industry. most prominent issue is public clamor protection general welfare sending a “chilling effect” “doubt” those engaged Also, jurisprudence different genetically modified organisms (GMOs) thereby biotechnology gene engineering limitations restrictions applications uses. These challenges issues, optimistic achieving standards perfection toward new beginning

Язык: Английский

Процитировано

0
Cas9-expressing cattle using all-in-one CRISPR/Cas9 for bovine genome editing DOI Creative Commons
Dong-Hyeok Kwon, Gyeong‐Min Gim, Soo‐Young Yum

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 12, 2024

Abstract Background Livestock, particularly cattle, are crucial for biotechnology fields, such as genetic breeding, infectious diseases, bioreactors, and specific disease models. However, engineering in cattle has lagged due to long gestation periods, single embryo pregnancies, high rearing costs. Additionally, the slow validation of germline transmission absence germline-competent embryonic stem cells hinder progress. With development genome editing technologies like ZFN, TALEN, CRISPR-Cas9, recent advancements have shown that Cas9-expressing pigs chickens been successfully produced. We hypothesize generating CRISPR/Cas9-expressing their resources will provide a powerful resource bovine editing, advancing our understanding genetics resistance. Results In this study, two types were produced: Cas9-RFP-FatI, Cas9-GFP-sgPRNP. Somatic from these induced mutate several target genes when single-guide RNAs (sgRNAs) transfected into somatic cells. semen CRISPR/Cas9 expressing male was frozen used fertilize wild-type oocytes, transmitting transgene (Cas9, reporter genes, fatty acid dehydrogenase I [FatI]), sgRNA prion protein [PRNP]) next generation. Furthermore, gene capabilities Cas9, including knockout high-efficiency knock-in, confirmed embryos derived F1 through vitro production. Conclusion These data demonstrate, first time, born, transmitted next-generation calves (F1) F2 embryos. addition, germ F0 F1generations evaluate potential (knockout knock-in) multiple genes. PRNP-mutated currently being raised resistance model spongiform encephalopathy. transgenic models derivatives serve valuable both vivo genomics diseases.

Язык: Английский

Процитировано

0