Cellular and Molecular Biology,
Год журнала:
2024,
Номер
70(1), С. 34 - 39
Опубликована: Янв. 31, 2024
Acute
lung
injury
(ALI)
is
associated
with
the
leukocyte
infiltration
and
inflammation.
Previous
studies
have
shown
that
miR-146a
a
valid
regulator
of
macrophage
polarization
in
vitro
inflammatory
model.
However,
it
unclear
whether
plays
protective
role
ALI
via
modulating
To
explore
potential
therapeutic
effect
mechanism
on
ALI.
We
analyzed
expression
acute
injured
tissues
differentiated
macrophage.
Lipopolysaccharide
(LPS)
interleukin-4
(IL-4)
were
employed
provoking
to
polarization.
used
mimics
improve
overexpression
investigated
increased
LPS-induced
mice
target
showed
markedly
decreased
tissue
type
M1
macrophage,
while
exhibited
M2
Moreover,
reversed
phenotype
anti-inflammatory
mitigated
level
inhibiting
Notch
1
signaling
pathway.
Hence,
inflammation,
infiltration,
integrity
capillary
barrier,
histology
model
corrected
after
treatment.
These
results
suggested
promotes
restraining
Overexpression
prevents
inflammation
damage
ameliorates
LPS
induction.
Therefore,
may
serve
as
promising
for
therapy
future.
Cellular Molecular and Biomedical Reports,
Год журнала:
2023,
Номер
3(2), С. 89 - 97
Опубликована: Апрель 11, 2023
MicroRNAs
(miRNA)
are
a
group
of
small
non-coding
RNAs
that
regulate
gene
expression
at
the
RNA
level.
have
positive
regulatory
effects
on
protein
translation
processes
and
often
induce
their
performance
by
binding
to
3'-UTR
mRNA
region.
Also,
microRNAs
involved
in
various
cellular
processes,
including
development,
cell
division,
signaling,
growth,
generally
play
an
effective
role
cycle
control
physiological
pathology.
Several
studies
confirm
important
initiation
progression
cancer,
many
them
act
as
oncogenes
tumor
suppressors.
On
other
hand,
stimulating
factors
can
biomarkers
diagnosis
prognosis
types
cases,
occurrence
mutations
open-reading
templates
lead
cancer.
also
regulating
expression.
Biological
shown
about
30%
all
genes
majority
genetic
pathways
regulated
microRNAs.
In
general,
target
molecules
potential
biological
goals
for
primary
screening,
targeted
treatment,
pharmaceutical
resistance,
identifying
provides
clear
prospect
better
understanding
leading
Oral
squamous
cell
carcinoma
(OSCC)
is
a
highly
aggressive
malignancy
with
poor
prognosis,
mainly
due
to
the
presence
of
cancer
stem
cells
(CSCs),
small
subpopulation
that
contribute
therapy
resistance
and
tumor
progression.
The
principal
objective
this
study
was
investigate
role
miRNA-21
in
maintenance
stemness
possibility
altering
it.
CD44
antigen
used
as
marker
for
CSC
isolation
from
oral
cultures.
CD44+
CD44-
populations
were
sorted
via
magnetic
separation.
inhibition
performed
transfection.
possessed
significantly
higher
migration
invasion
potential
compared
cells,
levels
(p
=
0.004)
β-catenin
0.005),
lower
BAX
0.015).
reduced
migration,
invasion,
colony
formation
while
increasing
apoptosis.
Stemness
markers
downregulated
following
inhibition:
OCT4
0.013),
SOX2
0.008),
NANOG
0.0001),
well
gene
(CTNNB1)
<
0.05),
an
important
member
WNT
signaling
pathway.
Apoptotic
activity
enhanced,
significant
downregulation
antiapoptotic
Bcl-2
0.008)
gene.
In
conclusion,
plays
critical
regulation
properties.
Targeting
inhibiting
could
represent
promising
novel
strategy
OSCC
treatment.
BMC Cardiovascular Disorders,
Год журнала:
2024,
Номер
24(1)
Опубликована: Апрель 23, 2024
Abstract
In
this
study,
we
sought
to
investigate
the
mechanisms
of
action
miR-195-5p
in
osteogenic
differentiation
vascular
smooth
muscle
cells
(VSMCs),
and
thereby
provide
novel
insights
a
reference
for
targeted
therapy
arterial
media
calcification.
VSMC
was
induced
using
sodium
β-glycerophosphate,
investigated
effects
transfecting
with
mimics,
vectors
overexpressing
Smad7,
Wnt/β-catenin
pathway
inhibitor
(KYA1797K)
on
by
determining
cell
viability
apoptosis,
mRNA
protein
expression
factors
associated
pathway.
The
results
revealed
that
mimics
enhanced
VSMCs
whereas
overexpression
Smad7
reversed
phenomenon.
addition,
KYA1797K
found
promote
overexpression.
conclusion,
targeting,
promotes
thus
VSMCs.
These
findings
will
elucidating
whereby
regulates
differentiation.
