In silico determination of potential miRNAs encoded in wheat genome that target AvrSr35 in Puccinia graminis f. sp. tritici DOI Creative Commons
Aydın Atakan,

Hayri Mert Motorcu,

S. Marakli

и другие.

International Journal of Pioneering Technology and Engineering, Год журнала: 2024, Номер 3(01), С. 27 - 30

Опубликована: Июнь 26, 2024

Wheat (Triticum aestivum L.) belonging to Poaceae family is a valuable staple food all over the world. Puccinia graminis f. sp. tritici one of major limiting factor in wheat production, causing stem rust disease. RNA interference (RNAi) an effective technique for plant production against abiotic stresses. One most investigated RNAi molecules microRNAs (miRNAs). It well established that miRNAs play important role regulation gene expression at post-transcriptional and pos-translational level. In present study, miRNAs-stem interactions were by silico methods. For this purpose, reference mature retrieved from miRBase, sequences avirulence (AvrSr35) obtained NCBI. RNAhybrid algorithm was used predict relationships between targets. Moreover, phylogenetic tree constructed using via MEGA X. We determined AvrSr35 targeted total 12 including tae-miR9657a-3p, tae-miR9671-5p, tae-miR1122c-3p, tae-miR1130b-3p, tae-miR9678-3p, tae-miR9781, tae-miR9666b-3p, tae-miR531, tae-miR9773, tae-miR9778, tae-miR9677b tae-miR10516. All separated into three groups. showed close relationship. The first main group consisted only tae-miR9778. Obtaining findings are expected contribute roles interaction disease-related plants pathogens.

Язык: Английский

In silico determination of potential miRNAs encoded in wheat genome that target AvrSr35 in Puccinia graminis f. sp. tritici DOI Creative Commons
Aydın Atakan,

Hayri Mert Motorcu,

S. Marakli

и другие.

International Journal of Pioneering Technology and Engineering, Год журнала: 2024, Номер 3(01), С. 27 - 30

Опубликована: Июнь 26, 2024

Wheat (Triticum aestivum L.) belonging to Poaceae family is a valuable staple food all over the world. Puccinia graminis f. sp. tritici one of major limiting factor in wheat production, causing stem rust disease. RNA interference (RNAi) an effective technique for plant production against abiotic stresses. One most investigated RNAi molecules microRNAs (miRNAs). It well established that miRNAs play important role regulation gene expression at post-transcriptional and pos-translational level. In present study, miRNAs-stem interactions were by silico methods. For this purpose, reference mature retrieved from miRBase, sequences avirulence (AvrSr35) obtained NCBI. RNAhybrid algorithm was used predict relationships between targets. Moreover, phylogenetic tree constructed using via MEGA X. We determined AvrSr35 targeted total 12 including tae-miR9657a-3p, tae-miR9671-5p, tae-miR1122c-3p, tae-miR1130b-3p, tae-miR9678-3p, tae-miR9781, tae-miR9666b-3p, tae-miR531, tae-miR9773, tae-miR9778, tae-miR9677b tae-miR10516. All separated into three groups. showed close relationship. The first main group consisted only tae-miR9778. Obtaining findings are expected contribute roles interaction disease-related plants pathogens.

Язык: Английский

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