Preparation and Optimization of Gemcitabine Loaded PLGA Nanoparticle Using Box-Behnken Design for Targeting to Brain: In Vitro Characterization, Cytotoxicity and Apoptosis Study DOI
Ladi Alik Kumar, Gurudutta Pattnaik, Bhabani Sankar Satapathy

и другие.

Current Nanomaterials, Год журнала: 2023, Номер 9(4), С. 324 - 338

Опубликована: Дек. 27, 2023

Background: Treatment of glioma with conventional approaches remains a far-reaching target to provide the desired outcome. This study aimed develop and optimize Gemcitabine hydrochloride- loaded PLGA nanoparticles (GNPs) using Box-Behnken design methodology. The independent variables chosen for this included quantity Polymer (PLGA) (X1), Tween 80 (X2), Sonication time (X3), whereas dependent were Particle size (Y1) EE % (Y2) PDI (Y3). optimized biodegradable investigated their anticancer effectiveness in U87MG human glioblastoma cells vitro. Method: formulation process involved two steps. Initially, emulsification was carried out by combining organic polymer solution aqueous surfactant solution. Subsequently, second step, solvent evaporated, resulting precipitation formation nanoparticles. PLGA, 80, PVA (at constant concentration) adjusted based on experimental trial approach. PLGA-based underwent characterization, wherein particle size, encapsulation efficiency, polydispersity index (PDI), cumulative release assessed. optimal composition determined as 200 mg 4 ml 2 PVA. Further, GNPs evaluated anti-cancer U87 MG MTT apoptosis assay. Results: results demonstrated that exhibited an efficiency 81.66 %, 140.1 nm, 0.37. morphology Opt-GNPs observed be spherical through transmission electron microscopy (TEM). Conclusion: Apoptosis further confirmed observations assay Opt- significantly enhanced U-87 than Standard marketed formulation.

Язык: Английский

Nanobilosome as custom-tailored modality in addressing Alzheimer’s disease: advancements and translational glitches DOI

Biswabhusan Biswal,

Bhabani Sankar Satapathy, Laxmidhar Maharana

и другие.

Journal of Dispersion Science and Technology, Год журнала: 2025, Номер unknown, С. 1 - 32

Опубликована: Апрель 11, 2025

Язык: Английский

Процитировано

0
Preparation and Optimization of Gemcitabine Loaded PLGA Nanoparticle Using Box-Behnken Design for Targeting to Brain: In Vitro Characterization, Cytotoxicity and Apoptosis Study DOI
Ladi Alik Kumar, Gurudutta Pattnaik, Bhabani Sankar Satapathy

и другие.

Current Nanomaterials, Год журнала: 2023, Номер 9(4), С. 324 - 338

Опубликована: Дек. 27, 2023

Background: Treatment of glioma with conventional approaches remains a far-reaching target to provide the desired outcome. This study aimed develop and optimize Gemcitabine hydrochloride- loaded PLGA nanoparticles (GNPs) using Box-Behnken design methodology. The independent variables chosen for this included quantity Polymer (PLGA) (X1), Tween 80 (X2), Sonication time (X3), whereas dependent were Particle size (Y1) EE % (Y2) PDI (Y3). optimized biodegradable investigated their anticancer effectiveness in U87MG human glioblastoma cells vitro. Method: formulation process involved two steps. Initially, emulsification was carried out by combining organic polymer solution aqueous surfactant solution. Subsequently, second step, solvent evaporated, resulting precipitation formation nanoparticles. PLGA, 80, PVA (at constant concentration) adjusted based on experimental trial approach. PLGA-based underwent characterization, wherein particle size, encapsulation efficiency, polydispersity index (PDI), cumulative release assessed. optimal composition determined as 200 mg 4 ml 2 PVA. Further, GNPs evaluated anti-cancer U87 MG MTT apoptosis assay. Results: results demonstrated that exhibited an efficiency 81.66 %, 140.1 nm, 0.37. morphology Opt-GNPs observed be spherical through transmission electron microscopy (TEM). Conclusion: Apoptosis further confirmed observations assay Opt- significantly enhanced U-87 than Standard marketed formulation.

Язык: Английский

Процитировано

4