A Highly Sensitive Electrochemical Immunosensor for Cortisol Detection DOI Creative Commons
Pritu Parna Sarkar, Ali Ashraf, Ahmed Hasnain Jalal

и другие.

Biosensors, Год журнала: 2025, Номер 15(5), С. 321 - 321

Опубликована: Май 17, 2025

In this research, an interdigitated gear-shaped working electrode is presented for cortisol sensing. Overall, the sensor was designed in a three-electrode system and fabricated using direct laser scribing. A synthesized conductive ink based on graphene polyaniline further employed to enhance electrochemical performance of sensor. Scanning electron microscopy (SEM) Fourier transform infrared (FTIR) spectroscopy were physicochemical characterization laser-induced (LIG) Cortisol, biomarker essential detecting stress, detected both phosphate-buffered saline (PBS, pH = 7.4) human serum within linear range 100 ng/mL µg/mL. Ferri/ferrocyanide as redox probe detect PBS. The developed assessed via differential pulse voltammetry (DPV), cyclic (CV), impedance (EIS), chronoamperometry. demonstrates high sensitivity selectivity alongside strong repeatability (relative standard deviation (RSD) 3.8%, n 4) reproducibility (RSD 5.85%, 5). these results highlight sensor’s reliability, sensitivity, detection cortisol. successfully complex medium effectively distinguished it ternary mixture containing dopamine. Also, use writing Kapton film makes approach cost-effective thus disposable, making suitable chronic stress diagnostics neurological research applications.

Язык: Английский

A Highly Sensitive Electrochemical Immunosensor for Cortisol Detection DOI Creative Commons
Pritu Parna Sarkar, Ali Ashraf, Ahmed Hasnain Jalal

и другие.

Biosensors, Год журнала: 2025, Номер 15(5), С. 321 - 321

Опубликована: Май 17, 2025

In this research, an interdigitated gear-shaped working electrode is presented for cortisol sensing. Overall, the sensor was designed in a three-electrode system and fabricated using direct laser scribing. A synthesized conductive ink based on graphene polyaniline further employed to enhance electrochemical performance of sensor. Scanning electron microscopy (SEM) Fourier transform infrared (FTIR) spectroscopy were physicochemical characterization laser-induced (LIG) Cortisol, biomarker essential detecting stress, detected both phosphate-buffered saline (PBS, pH = 7.4) human serum within linear range 100 ng/mL µg/mL. Ferri/ferrocyanide as redox probe detect PBS. The developed assessed via differential pulse voltammetry (DPV), cyclic (CV), impedance (EIS), chronoamperometry. demonstrates high sensitivity selectivity alongside strong repeatability (relative standard deviation (RSD) 3.8%, n 4) reproducibility (RSD 5.85%, 5). these results highlight sensor’s reliability, sensitivity, detection cortisol. successfully complex medium effectively distinguished it ternary mixture containing dopamine. Also, use writing Kapton film makes approach cost-effective thus disposable, making suitable chronic stress diagnostics neurological research applications.

Язык: Английский

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