Exploring the Antibacterial Potential of Bile Salts: Inhibition of Biofilm Formation and Cell Growth in Pseudomonas aeruginosa and Staphylococcus aureus DOI Creative Commons
Anuradha Tyagi, Vinay Kumar, Navneet Joshi

и другие.

Microbiology Research, Год журнала: 2024, Номер 15(3), С. 1269 - 1279

Опубликована: Июль 21, 2024

Chronic infections often involve notorious pathogens like Pseudomonas aeruginosa and Staphylococcus aureus, demanding innovative antimicrobial strategies due to escalating resistance. This investigation scrutinized the antibacterial prowess of bile salts, notably taurocholic acid (TCA), ursodeoxycholic (UDCA), ox salt (OBS), against these pathogens. Evaluations encompassed minimum inhibitory concentration (MIC) determination, scrutiny their impact on biofilm formation, anti-virulence mechanisms. UDCA exhibited highest efficacy, suppressing S. aureus P. biofilms by 83.5% 78%, respectively, at peak concentration. TCA also significantly reduced development 81% for 75% aeruginosa. Microscopic analysis revealed substantial disruption architecture TCA. Conversely, OBS demonstrated ineffectiveness both Mechanistic assays elucidated TCA’s detrimental cell membrane, prompting release macromolecular compounds. Additionally, inhibited protease elastase synthesis in staphyloxanthin lipase production aureus. These results underscore potential impeding formation mitigating pathogenicity

Язык: Английский

Exploring the Antibacterial Potential of Bile Salts: Inhibition of Biofilm Formation and Cell Growth in Pseudomonas aeruginosa and Staphylococcus aureus DOI Creative Commons
Anuradha Tyagi, Vinay Kumar, Navneet Joshi

и другие.

Microbiology Research, Год журнала: 2024, Номер 15(3), С. 1269 - 1279

Опубликована: Июль 21, 2024

Chronic infections often involve notorious pathogens like Pseudomonas aeruginosa and Staphylococcus aureus, demanding innovative antimicrobial strategies due to escalating resistance. This investigation scrutinized the antibacterial prowess of bile salts, notably taurocholic acid (TCA), ursodeoxycholic (UDCA), ox salt (OBS), against these pathogens. Evaluations encompassed minimum inhibitory concentration (MIC) determination, scrutiny their impact on biofilm formation, anti-virulence mechanisms. UDCA exhibited highest efficacy, suppressing S. aureus P. biofilms by 83.5% 78%, respectively, at peak concentration. TCA also significantly reduced development 81% for 75% aeruginosa. Microscopic analysis revealed substantial disruption architecture TCA. Conversely, OBS demonstrated ineffectiveness both Mechanistic assays elucidated TCA’s detrimental cell membrane, prompting release macromolecular compounds. Additionally, inhibited protease elastase synthesis in staphyloxanthin lipase production aureus. These results underscore potential impeding formation mitigating pathogenicity

Язык: Английский

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