Comparative evaluation of three anti-dsDNA antibody detection methods in systemic lupus erythematosus: insights from a large monocentric cohort

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Апрель 10, 2025

Anti-double-stranded DNA (anti-dsDNA) antibodies at abnormal titer are of considerable diagnostic value for systemic lupus erythematosus (SLE). Current assays detecting anti-dsDNA show divergent properties, emphasizing the importance selecting suitable assays. This study aims to investigate performance indirect immunofluorescence (IIF), digital liquid chip method (DLCM), chemiluminescence immunoassay (CLIA), and their combinations in SLE. We conducted a retrospective, single-center from 2022 2023 which included 3429 samples: 1773 patients with SLE 1656 controls rheumatoid arthritis (RA) Sjögren's syndrome (SS). Sensitivity, specificity, accuracy, positive predictive (PPV), negative (NPV) detection by IIF, DLCM, CLIA were calculated. Cohen's kappa coefficient was used evaluate inter-method agreement. The correlations between concentration SLEDAI-2k scores/renal involvement assessed. Among individual assays, IIF demonstrated highest specificity (98.31%) PPV (96.10%) but lower sensitivity (38.92%) compared (41.57%) DLCM (43.65%) (p < 0.05). Combining two significantly improved while maintaining specificity>95%. combination achieved 52.2% an AUC 0.76. Substantial agreement observed (κ = 0.78), whereas other moderate 0.65-0.66). In longitudinal analysis 88 patients, detected antibody fluctuations more reliably than IIF. Anti-dsDNA levels or positively correlated SLEDAI-2K scores (R=0.42 0.29, p<0.05). Both methods showed significant differences without renal provided higher single (p<0.001) subgroups. Our findings demonstrate that performs comparably CLIA, supporting its clinical potential. Moreover, combining enhances sensitivity, particularly subgroups involvement.

Язык: Английский

Clinical Performance of the Line Immunoassay and Digital Liquid Chip Method for Detecting Autoimmune Liver Disease Autoantibodies

Archives of Pathology & Laboratory Medicine, Год журнала: 2024, Номер unknown

Опубликована: Июнь 4, 2024

Context.— The identification of autoantibodies associated with autoimmune liver disease (ALD) is crucial for diagnosis and management. Various laboratory methods have been introduced to detect autoantibody profiles. However, the variable performance these assays may create challenges clinicians patients. Objective.— To investigate concordance rates diagnostic 2 commercially available assays, line immunoassay (LIA) digital liquid chip method (DLCM), in patients ALD. Design.— A total 291 serum samples were collected, consisting 180 sera from ALD 111 controls. detected through LIA DLCM. agreement each assay analyzed. Results.— There was substantial almost perfect among prevalent (anti–mitochondrial antibody M2, antibodies against gp210, Sp100, Ro52). Nevertheless, Cohen κ coefficient some uncommon (anti–LKM-1, anti–LC-1, anti-SLA/LP) between not ideal. showed slightly better sensitivity, accuracy, negative predictive value, while DLCM exhibited higher specificity positive value. Conclusions.— demonstrated comparable detection common ALD-related autoantibodies. seemed be more sensitive, displayed specificity. standardization still faces diverse systems. Comprehensive interlaboratory validation essential mitigate potential misunderstanding confusion clinicians.

Язык: Английский

Newer Autoantibodies and Laboratory Assessments in Myositis

Current Rheumatology Reports, Год журнала: 2024, Номер 27(1)

Опубликована: Дек. 5, 2024

Abstract Purpose of Review We aim to describe the immunoassays that have been used for myositis autoantibody discovery with a focus on newer methods. recently identified autoantibodies do not yet form part routine clinical testing, highlighting what is known about their associated phenotype and potential clues as presence. Recent Findings Novel approaches detection employed in recent years including chemiluminescent immunoassay, phage immunoprecipitation-sequencing modifications more traditional immunoprecipitation technique. This has led novel autoantibodies, anti-aminoacyl-tRNA synthetase which modify cancer risk patients anti-TIF1ɣ dermatomyositis. Summary New facilitated will enable identification broader range autoantigens. Challenges remain translating this knowledge into accessible testing particularly given rarity most discovered autoantibodies.

Язык: Английский