REST Promotes Autophagy in Gastric Cancer by Transcriptionally Activating FABP6 to Inhibit the Akt/mTOR Signaling Pathway
Frontiers in Bioscience-Landmark,
Год журнала:
2024,
Номер
29(6), С. 212 - 212
Опубликована: Июнь 12, 2024
Background:
Gastric
cancer
(GC)
is
a
leading
cause
of
cancer-associated
death
worldwide.
Its
molecular
mechanisms,
especially
concerning
autophagy
and
various
signaling
pathways,
are
not
fully
understood.
Fatty
Acid
Binding
Protein
6
(FABP6)
RE1
Silencing
Transcription
Factor
(REST)
emerge
as
potential
key
players
in
this
context.
This
study
sought
to
analyze
the
functional
relationship
FABP6
REST
their
implications
on
Akt/mTOR
pathway
within
GC
cells.
Methods:
A
comprehensive
bioinformatics
approach
was
used
identify
prognostic
markers
GC.
The
effects
along
with
pathways
were
analyzed
by
techniques
including
Western
blotting
(WB),
flow
cytometry,
Transwell
assay,
dual
luciferase
reporter
others.
Results:
identified
overexpressed
GC,
linked
poor
prognosis.
silencing
reduces
cell
proliferation,
induces
S-
G2-phase
arrest,
downregulates
cyclins
CDK2
CDK4.
It
also
inhibited
invasion/migration
autophagy,
that
counteracted
MG132.
When
combined
PI3K
inhibitor
LY294002c,
knockdown
showed
synergistic
anti-proliferative
effects,
modulating
pathway.
Besides,
transcription
factor
has
been
shown
directly
regulate
expression,
affecting
FABP6-dependent
manner.
Conclusions:
positively
regulates
negatively
affects
cells
manner,
providing
valuable
insights
into
regulatory
networks
involving
REST.
Язык: Английский
Transcription factor <i>ASCL1</i> targets <i>SLC6A13</i> to inhibit the progression of hepatocellular carcinoma via the glycine-inflammasome signaling
Biomolecules and Biomedicine,
Год журнала:
2024,
Номер
24(6), С. 1606 - 1619
Опубликована: Май 21, 2024
Hepatocellular
carcinoma
(HCC),
the
most
common
primary
liver
cancer,
typically
arises
from
chronic
conditions
such
as
hepatitis,
cirrhosis,
or
other
diseases,
and
is
characterized
by
its
aggressive
nature
poor
prognosis.
The
purpose
of
this
research
was
to
clarify
function
achaete-scute
family
bHLH
transcription
factor
1
(ASCL1)
solute
carrier
6
member
13
(SLC6A13)
in
influencing
tumor
cell
behavior,
inflammatory
responses,
regulation
inflammasomes.
We
analyzed
differentially
expressed
genes
(DEGs)
Cancer
Genome
Atlas-Liver
Carcinoma
(TCGA-LIHC)
database,
well
GSE14520
GSE67764
datasets,
identify
expression
changes
SLC6A13
cancer.
prognostic
significance
LIHC
assessed
through
Kaplan-Meier
survival
curve
analysis.
Transcriptional
ASCL1
explored
using
Joint
Annotation
Human
species
Systematic
Pipeline
for
Regulatory
Regions
(JASPAR)
database
dual-luciferase
assays.
In
vitro
experiments
investigated
impact
overexpression
on
HCC
growth.
Additionally,
effects
ethanol
treatment
glycine
modulation
response
lines
were
evaluated.
samples
showed
reduced
levels
SLC6A13,
which
correlates
with
a
better
prognosis
metastases.
Elevated
correlated
improved
overall
(OS),
progression-free
(PFS),
recurrence-free
(RFS),
disease-specific
(DSS).
upregulated
inhibited
proliferation,
migration,
invasion
cells.
Ethanol
induced
production
cytokines,
enhanced
but
counteracted
glycine.
This
study
highlighted
elevated
has
been
OS,
PFS,
RFS,
DSS.
Overexpression
cells
inflammasome
activation,
exacerbated
attenuated
Язык: Английский