Therapeutic Potential of Umbilical Cord MSC-Derived Exosomes in a Severe Dry Eye Rat Model: Enhancing Corneal Protection and Modulating Inflammation DOI Creative Commons

Sze-Min Chan,

Chris Tsai,

Tai-Ping Lee

и другие.

Biomedicines, Год журнала: 2025, Номер 13(5), С. 1174 - 1174

Опубликована: Май 11, 2025

Background/Objectives: Dry eye disease (DED) is a multifactorial inflammatory that disrupts the ocular surface, causing tear film instability, epithelial damage, and chronic inflammation. Mesenchymal stem cell-derived exosomes (MSC-exos) are promising therapeutics with immunomodulatory regenerative properties. This study investigates therapeutic effects of umbilical cord MSC-derived (UCMSC-exos) in severe dry model, induced by surgical resection infra-orbital (ILG) extra-orbital lacrimal gland (ELG) rats. Methods: Clinical evaluations, including volume measurement, slit lamp biomicroscopy, fluorescein staining, spectral domain optical coherence tomography (SD-OCT), were performed to assess corneal neovascularization, abrasion, epithelial/stromal thickness. Histopathological analysis, immunohistochemistry, mRNA gene expression conducted evaluate tissue changes marker expression. Results: The results show treatment group exhibited significantly reduced neovascularization compared control (p = 0.030). During first month, Exo also had lower staining area 0.032), suggesting accelerated wound healing. SD-OCT analysis revealed thickness was closer normal levels 0.02 p 0.006, respectively). UCMSC-exos modulated α-SMA apoptosis cornea. Additionally, cytokines (IL-1β TNF-α) downregulated. Conclusions: These findings suggest MSC-exosome therapy offers novel, cell-free approach for managing DED, modulating response.

Язык: Английский

Extracellular vesicles: emerging therapeutic agents for liver fibrosis DOI Open Access
Giulia Chiabotto,

Armina Semnani,

Elena Ceccotti

и другие.

Extracellular Vesicles and Circulating Nucleic Acids, Год журнала: 2025, Номер 6(2), С. 216 - 44

Опубликована: Май 7, 2025

Liver fibrosis is a progressive condition characterized by excessive scar tissue buildup, leading to impaired liver function and potentially cirrhosis. Despite advancements in treatment strategies, effective anti-fibrotic therapies remain an urgent unmet need. Recent research has identified extracellular vesicles (EVs) as promising therapeutic agents due their ability mediate intercellular communication regulate key fibrotic pathways. This review aims provide comprehensive overview of the potential EVs different vitro vivo models hepatic fibrosis, focusing on natural effects recent engineering for enhanced efficacy. can be derived from various cellular sources, including mesenchymal stromal cells (MSCs) liver-resident cells. Biological materials, serum, breast milk, bacteria, plants, also serve EV sources. Among these, MSC-EVs stand out potential, which significantly through preconditioning with inflammatory signals, pharmacological agents, or genetic improve quality Engineering techniques have further expanded applications, enabling use precise drug-delivery vehicles. Approaches such loading compounds, designing customized EVs, creating EV-liposome hybrids enable targeted delivery activated stellate (HSCs), central drivers progression. These strategies enhance efficacy EV-based treatments. Both engineered critical pathways development, activation HSCs, modulation pro-fibrotic genes, matrix deposition, programmed cell death. Additionally, modulate immune responses, fostering microenvironment conducive repair regeneration. Combining regenerative properties innovative provides highly targeted, approaches restore address need chronic disease therapies.

Язык: Английский

Процитировано

0

Therapeutic Potential of Umbilical Cord MSC-Derived Exosomes in a Severe Dry Eye Rat Model: Enhancing Corneal Protection and Modulating Inflammation DOI Creative Commons

Sze-Min Chan,

Chris Tsai,

Tai-Ping Lee

и другие.

Biomedicines, Год журнала: 2025, Номер 13(5), С. 1174 - 1174

Опубликована: Май 11, 2025

Background/Objectives: Dry eye disease (DED) is a multifactorial inflammatory that disrupts the ocular surface, causing tear film instability, epithelial damage, and chronic inflammation. Mesenchymal stem cell-derived exosomes (MSC-exos) are promising therapeutics with immunomodulatory regenerative properties. This study investigates therapeutic effects of umbilical cord MSC-derived (UCMSC-exos) in severe dry model, induced by surgical resection infra-orbital (ILG) extra-orbital lacrimal gland (ELG) rats. Methods: Clinical evaluations, including volume measurement, slit lamp biomicroscopy, fluorescein staining, spectral domain optical coherence tomography (SD-OCT), were performed to assess corneal neovascularization, abrasion, epithelial/stromal thickness. Histopathological analysis, immunohistochemistry, mRNA gene expression conducted evaluate tissue changes marker expression. Results: The results show treatment group exhibited significantly reduced neovascularization compared control (p = 0.030). During first month, Exo also had lower staining area 0.032), suggesting accelerated wound healing. SD-OCT analysis revealed thickness was closer normal levels 0.02 p 0.006, respectively). UCMSC-exos modulated α-SMA apoptosis cornea. Additionally, cytokines (IL-1β TNF-α) downregulated. Conclusions: These findings suggest MSC-exosome therapy offers novel, cell-free approach for managing DED, modulating response.

Язык: Английский

Процитировано

0