Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)
Опубликована: Апрель 3, 2025
Язык: Английский
Health Science Reports, Год журнала: 2024, Номер 7(8)
Опубликована: Авг. 1, 2024
Язык: Английский
Процитировано
41
Clinical and Experimental Medicine, Год журнала: 2025, Номер 25(1)
Опубликована: Март 6, 2025
Graft-versus-host disease remains one of the most formidable barriers to complete success hematopoietic stem cell transplantation that has emerged as curative approach for many malignancies because it affects quality life and overall survival. Macrophages are among important members immune system, which perform dual roles in GVHD both therapeutic tools targets. This review epitomizes multifunctional role macrophages pathophysiology acute chronic GVHD. play an early phase their recruitment infiltration into target organs. Furthermore, they polarize two functionally different phenotypes, including M1 M2. In case GVHD, express phenotype characterized by production pro-inflammatory cytokines contribute tissue damage. contrast, tend toward M2 associated with repair tissues fibrosis. A critical balance these phenotypes is central course severity Further interactions other lymphocytes such T cells, B fibroblast further determine Macrophage interaction alloreactive cells promotes inflammation. therefore inducing injuries during Interaction macrophages, cell, fibroblast, CD4+ fibrosis and, hence, subsequent dysfunction These some insights, while several challenges remain. First, impact dominant on polarization incompletely sometimes controversial. Second, development targeted therapies able modulate macrophage function without systemic side effects area ongoing investigation. Future directions involve exploration macrophage-targeted therapies, small molecules, antibodies, nanotechnology, behavior improve patient outcomes. underlines fact a profound understanding essential developing new more effective strategies. Targeting might represent avenue decreasing incidence improving safety HSCT.
Язык: Английский
Процитировано
7
Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)
Опубликована: Март 17, 2025
Abstract Recent breakthroughs in tumor immunotherapy have confirmed the capacity of immune system to fight several cancers. The effective means treating cancer involves accelerating death cells and improving patient immunity. Dynamic changes microenvironment alter actual effects anti-tumor drug production may trigger favorable or unfavorable responses by modulating tumor-infiltrating lymphocytes. Notably, CD8 + T are one primary that provide response. Tumor stem will resist evade destruction through various mechanisms as exert their function. This paper reviews research on regulation development prognosis directly indirectly role cells. We also discuss related strategies.
Язык: Английский
Процитировано
4
Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)
Опубликована: Янв. 23, 2025
The colon and rectum are highly innervated, with neural components within the tumor microenvironment playing a significant role in colorectal cancer (CRC) progression. While perineural invasion (PNI) is associated poor prognosis CRC, impact of nerve density diameter on behavior remains unclear. This study aims to evaluate prognostic value characteristics CRC verify nerves growth. Tissue samples from 129 patients were stained immunofluorescent markers NF-L S100B detect nerves. Nerve measured normalized. Kaplan-Meier survival analysis Cox regression models used identify factors. Prognostic established using receiver operating characteristic (ROC) curve assess predictive A murine chemical denervation model was employed disrupt sympathetic 6-hydroxydopamine, inhibit muscarinic receptor 3 darifenacin, ablate sensory neurons capsaicin. total 0.72 ± 0.59/mm², intratumoral (0.42 0.40/mm²) being significantly lower than extratumoral regions (1.00 0.75/mm²). average 28.14 6.04 μm, no difference between (28.2 7.65 μm) (27.86 6.72 μm). PNI observed 65 (50.4%). high normalized (NND) shorter overall disease-free patients, identified as an independent factor. Patients exhibit higher NND. Incorporating NND into ROC improved sensitivity specificity predictions. In model, sympathetic, parasympathetic, reduced rectal volume. critical factors influencing patient enhance accuracy prediction models. Moreover, effectively inhibits growth vivo, highlighting potential targeting therapeutic strategy CRC.
Язык: Английский
Процитировано
2
Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)
Опубликована: Апрель 10, 2025
Systems biology is a holistic approach to biological sciences that combines experimental and computational strategies, aimed at integrating information from different scales of processes unravel pathophysiological mechanisms behaviours. In this scenario, high-throughput technologies have been playing major role in providing huge amounts omics data, whose integration would offer unprecedented possibilities gaining insights on diseases identifying potential biomarkers. the present review, we focus strategies applied literature integrate genomics, transcriptomics, proteomics, metabolomics year range 2018-2024. Integration approaches were divided into three main categories: statistical-based approaches, multivariate methods, machine learning/artificial intelligence techniques. Among them, statistical (mainly based correlation) ones with slightly higher prevalence, followed by learning Integrating multiple layers has shown great uncovering molecular mechanisms, putative biomarkers, aid classification, most time resulting better performances when compared single analyses. However, significant challenges remain. The nature platforms introduces issues such as variable data quality, missing values, collinearity, dimensionality. These further increase combining datasets, complexity heterogeneity integration. We report found cope these challenges, but some open still remain should be addressed disclose full
Язык: Английский
Процитировано
2
Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)
Опубликована: Апрель 10, 2025
Ischemic stroke is a prevalent form of cerebrovascular accident, with its pathogenesis involving the intricate interplay between neuroinflammation and energy metabolism. Cerebral ischemia disrupts oxygen supply, triggering metabolic dysregulation activating neuroinflammatory responses, ultimately resulting in cellular damage. This review provides an exhaustive analysis complex mechanisms ischemic stroke, particular focus on interaction The interruption supply due to cerebral initiates activates including release inflammatory cytokines activation immune cells, contributing damage further disturbances. Studies indicate that metabolism significantly impairs neural cell function interacts neuroinflammation, exacerbating brain injury. Therapeutic strategies primarily concentrate modulating suppressing emphasizing importance in-depth research into their provide theoretical foundation for new treatment stroke. Future should how balance anti-inflammatory regulation minimize promote recovery.
Язык: Английский
Процитировано
1
Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)
Опубликована: Апрель 24, 2025
Metabolic Associated Steatotic Liver Disease (MASLD), encompassing conditions simple liver steatosis (MAFL) and metabolic associated steatohepatitis (MASH), is the most prevalent chronic disease. Currently, management of MASLD impeded by lack reliable diagnostic biomarkers effective therapeutic strategies. We analyzed eight independent clinical datasets from GEO database. Differential expression weighted gene co-expression network analyses (WGCNA) were used to identify 23 genes related inflammation. Five hub selected using machine learning techniques (SVM-RFE, LASSO, RandomForest) combined with a literature review. Nomograms created predict incidence, potential was evaluated through receiver operating characteristic (ROC) curves. Additionally, Protein-Protein Interaction (PPI) networks, functional enrichment, immune infiltration performed. Potential transcription factors agents also explored. Finally, biological significance these validated animal model, histological examination transcriptomic profiles. identified five genes-UBD/FAT10, STMN2, LYZ, DUSP8, GPR88-that are for MASLD. These exhibited strong potential, either individually or in combination. This study highlights key as promising candidates understanding findings offer new insights into disease's pathophysiology may contribute development better approaches.
Язык: Английский
Процитировано
1
Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)
Опубликована: Янв. 16, 2025
Colorectal cancer (CRC) remains a major global health challenge, with high rates of incidence and mortality. This study investigates the role protein arginine methyltransferase 6 (PRMT6) as an oncogene in CRC its mechanistic involvement tumor progression. We found that PRMT6 is significantly overexpressed tissues compared to adjacent normal associated poorer patient survival. Functional assays demonstrated promotes cell proliferation, migration, invasion. Mechanistically, enhances MYC signaling by stabilizing c-MYC through mono-methylation at 371, which inhibits poly-ubiquitination subsequent degradation. post-translational modification crucial for PRMT6-induced proliferation. Xenograft models further validated knockdown results reduced growth decreased levels. Our findings highlight key regulator stability progression, suggesting targeting or effects on could offer promising strategy treatment.
Язык: Английский
Процитировано
0
International Immunopharmacology, Год журнала: 2025, Номер 148, С. 114013 - 114013
Опубликована: Янв. 18, 2025
Язык: Английский
Процитировано
0
Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)
Опубликована: Фев. 21, 2025
Gastrointestinal (GI) cancers account for more than one-third of cancer-related mortality, and the prognosis late-stage patients remains poor. Immunotherapy has been proven to extend survival at advanced stages; however, challenges persist in patient selection overcoming drug resistance. Tumor-infiltrating lymphocytes (TILs) tertiary lymphoid structures (TLS) tumor microenvironment (TME) have found be associated with anti-tumor immune responses. 'Hot tumors' high levels infiltration tend respond better checkpoint inhibitor (ICI) therapy, making them potential biomarkers ICI treatment. To explore predicting immunotherapy response GI cancers, we downloaded gene expression profiles seven from The Cancer Genome Atlas (TCGA) database characterized their TME, classifying samples into hot/cold subgroups. Furthermore, developed a computational framework construct cancer-specific hot classification models only few genes. External independent datasets qPCR experiments were used verify performance our few-gene models. We constructed identify tumors two nine results showed that B cells are important determination, identified significantly TLS. They not overexpress TLS marker genes but also presence whole-slide images. Further, two-gene model was effectively distinguish between cold subgroups cholangiocarcinoma, providing an opportunity stratifying clinical settings. In conclusion, established models, which can easily integrated practice, subgroups, may serve as response.
Язык: Английский
Процитировано
0