Enhanced Osteoporosis Treatment via Nano Drug Coating Encapsulating Lactobacillus rhamnosus GG

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

Osteoporosis is the most common systemic skeletal disorder, particularly associated with aging and postmenopausal women. With growing knowledge about gut–bone axis, therapeutic strategies for osteoporosis have been shifted toward regulating gut microbiota to promote positive bone metabolism. AlthoughLactobacillus rhamnosus GG (LGG) widely reported positively regulate metabolism by restoring dysbiotic microbiome, oral administration sensitivity gastric fluid low bioavailability. Other studies also demonstrated that bisphosphonates ameliorate directly metabolism, especially inhibiting osteoclast activity. However, side effects caused bisphosphonate treatment still represent a significant problem. In this study, we assembled alendronate, clinically used bisphosphonate, DSPE-phospholipid nanoencapsulation LGG (ADB), protect from acidic environment of stomach, while simultaneously reducing gastrointestinal alendronate. We further investigated potential these nanoencapsulated bacteria ADB repair osteoporotic deterioration, their ability in vivo, which strongly intrinsic environment. Compared same dosage alendronate alone, regulated was significantly revised ovariectomized mice models.

Язык: Английский

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Stabilization of Osteoporotic Pelvis and Acetabular Fractures

Indian Journal of Orthopaedics, Год журнала: 2025, Номер 59(3), С. 300 - 310

Опубликована: Янв. 4, 2025

Язык: Английский

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Nature Herbal Medicine‐ Tissue Engineering Strategies for Regulate Cell Homeostasis in Bone Regeneration

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Янв. 10, 2025

Abstract Bone fractures, especially large and complex defects, continue to pose significant challenges in the medical field. Current treatments often rely on autografts or allografts for structural support, which can lead problems such as reduced bioactivity, infection risks, potential pathogen transmission. Nature herbal medicine (NHM), including herbs extracts, offers a promising alternative by effectively modulating inflammatory responses, enhancing osteoblast function, inhibiting bone resorption, thereby facilitating fracture repair. However, traditional drug delivery methods NHM encounter interactions, poor tissue distribution, patient compliance. Biomaterials, engineered interact with biological systems, play essential roles repair, mechanical delivery. When used carriers, biomaterials be combined form stable systems that further promote regeneration. Here applications of are reviewed, hydrogels, extracellular vesicles, cement, conjunction regulating homeostasis aiming provide valuable insights guidance future research therapeutic strategies.

Язык: Английский

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GelMA Hydrogels Integrated With aptamer CH6‐Functionalized Tetrahedral DNA Nanostructures for Osteoporotic Mandibular Regeneration

Macromolecular Bioscience, Год журнала: 2025, Номер unknown

Опубликована: Янв. 21, 2025

Osteoporotic bone regeneration is challenging due to impaired formation. Tetrahedral DNA nanostructures (TDN), promising nucleic acid nanomaterials, have garnered attention for their potential in osteoporotic mandibular owing ability enhance cellular activity and promote osteogenic differentiation. Osteoblasts play a critical role regeneration; however, intracellular delivery of TDN into osteoblasts remains difficult. In this study, novel osteoblast-targeted CH6 aptamer-functionalized (TDN-CH6) aimed develop regeneration. This results demonstrated that TDN-CH6 exhibits superior osteoblast specificity efficient recruitment fracture sites. Furthermore, significantly enhances differentiation compared alone. Notably, Gelatin Methacryloyl (GelMA) hydrogels incorporating shows improved biological performance are favorable regeneration, suggesting platform represents strategy addressing complex defects.

Язык: Английский

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Metal-Phenolic Modified Coaxial Electrospun Biomembrane Combined with the Photothermal Effect Enhances Bone Regeneration by Ameliorating Oxidative Stress and Mitochondrial Dysfunction via the PI3K/Akt Signaling Pathway

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

Critical-sized bone defect regeneration remains a significant clinical challenge due to the complex cascade of biological processes involved. To address this, we developed sophisticated hierarchical biomembrane (PCS@MPN10) designed modulate osteogenic microenvironment. Using coaxial electrospinning, fabricated core–shell structure with polylactic acid (PLA) as membrane base, incorporating simvastatin in core and chitosan shell. The surface was further modified tannic acid-iron metal-polyphenol network coating. Our results demonstrated that exhibits excellent biocompatibility, photothermal properties, antibacterial activity. Additionally, regulates microenvironment by promoting M1-to-M2 macrophage polarization, showing strong potential both vitro vivo. Furthermore, PCS@MPN10+NIR modulates mitochondrial function through PI3K-AKT pathway, clears reactive oxygen species (ROS), alleviates cellular oxidative stress, thereby enhancing regeneration. Overall, these findings suggest this holds great promise strategy for improving critical-sized defects.

Язык: Английский

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Physicochemical properties of magnetic scaffolds with hydroxyapatite nanoparticles and chitosan

MRS Advances, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

Язык: Английский

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ED-71 ameliorates OVX-induced osteoporosis by regulating calcium homeostasis and SIRT1-mediated mitochondrial function, alleviating osteoblast senescence and suppressing osteoclastogenesis

Cellular Signalling, Год журнала: 2025, Номер unknown, С. 111713 - 111713

Опубликована: Март 1, 2025

Язык: Английский

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Trifunctional Sialylation‐Based SF‐ZIF@NA Hydrogel for Selective Osteoclast Inhibition and Enhanced Bone‐Vessel Regeneration in Osteoporotic Bone Defects

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Март 26, 2025

Osteoporotic bone defects are challenging to repair due imbalances in resorption and formation, coupled with insufficient vascularization. To address these issues, it develops a trifunctional hydrogel (SF-ZIF@NA) designed selectively inhibit osteoclast activity enhance vascularized regeneration. By enzymatically removing sialic acid, SF-ZIF@NA prevents precursor osteoclasts (pOCs) from fusing into bone-resorbing mature (mOCs), thereby preserving pOCs their anabolic functions. Additionally, the releases Zinc ion (Zn2⁺) response acidic conditions, promoting osteogenesis angiogenesis. In vitro results confirmed that impedes fusion, enhances platelet-derived growth factor-BB (PDGF-BB secretion pOCs, activates FAK (focal adhesion kinase) signaling pathway stimulate formation. osteoporotic defect models, accelerated increased density These findings demonstrate offers targeted multifunctional strategy for regeneration by concurrently modulating

Язык: Английский

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Metal coordination-based double-network microsphere scaffolds facilitate bone regeneration via oxygen-driven mitochondrial oxidative phosphorylation

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 162233 - 162233

Опубликована: Апрель 1, 2025

Язык: Английский

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Multi‐Core Functional Microspheres for Osteoporotic Bone Defects Therapy: From Local Treatment to Systematic Relief

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

Abstract Bone repair in patients with osteoporosis is a major challenge. In this study, multi‐enhanced bioactive microsphere scaffolds (abbr. GMA MSs) composed of gelatin framework alendronate (ALN) and magnesium (Mg) spheres encapsulants are developed for repairing osteoporotic bone defects. The MSs prepared via the reverse microemulsion method photocuring process have uniform morphology. Hydrogen (H 2 ) generated from scavenged reactive oxygen species (ROS), regulated macrophage phenotype, inhibited phosphatidylinositol 3‐kinase/protein kinase B (PI3K/Akt) nuclear factor kappa‐B (NF‐κB) signaling pathways, decreased expression pro‐inflammatory factors, thereby improving inflammatory microenvironment. Concurrently, alkaline enhancement ALN release osteoclast activity, released Mg 2+ promoted osteogenic differentiation, which synergistically restored balance metabolism alongside ALN. After filling into skull defects, significantly improved response notable tissue defects ovariectomized (OVX) mice. Notably, local treatment relieved systemic reduced overall loss these mice, highlighting substantial potential scaffold clinical

Язык: Английский

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