Prevalence and Co‐Detection Rates of SARS‐CoV‐2, Influenza, and Respiratory Syncytial Virus: A Retrospective Analysis

Apmis, Год журнала: 2025, Номер 133(3)

Опубликована: Фев. 27, 2025

ABSTRACT In late 2022, there was a significant increase in the prevalence of RSV northeastern United States. This surge occurred concurrently with beginning traditional influenza season and ongoing circulation SARS‐CoV‐2. We retrospectively analyzed respiratory testing data at regional reference laboratory from September 2022 to April 2024 characterize incidence co‐detection RSV, A, B, SARS‐CoV‐2 The positivity rates were found be 16.68% for SARS‐CoV‐2, 11.66% 0.83% 9.11% during study period. Co‐detections observed 0.49% samples, SARS‐CoV‐2/influenza A being most common. less frequently than expected based on individual rates. During period, B increased over 10‐fold, decreased by 60%, more prevalent pediatric population.

Язык: Английский

---

Functional implications of respiratory syncytial virus F sequence variability: a comparative analysis using contemporary RSV isolates

mSphere, Год журнала: 2025, Номер unknown

Опубликована: Апрель 14, 2025

ABSTRACT Respiratory syncytial virus (RSV) remains a major global health issue. Therapeutic options are limited, but new prophylactics, all targeting the fusion (F) glycoprotein, were recently licensed. Although F sequence variation is it unclear if and how this variability translates to phenotypical differences. We analyzed full sequences of 105 contemporary RSV isolates, gathered from children presenting with acute lower respiratory tract infections. Based on antigenic site variation, 20 isolates selected for functional characterization. diversity generally low, overall mean pairwise distance between RSV-A strains higher than RSV-B (0.014 vs 0.008, P < 0.001). Sequence in known monoclonal antibody (mAb)-binding sites seems insufficient explain divergent often subgroup-dependent sensitivity mAb neutralization. Despite comparable growth rates, syncytium size was (mean 61.68 [SD 24.51] 31.72 11.64], 0.001), frequency 25.37 9.20] 47.85 [11.52], 0.001) strains. For temperatures associated increased inactivation, although some displayed manifestly stability. Genomic phenotypic among our noticeable exceptions. The availability prophylactics corroborates importance continued surveillance identify changes sequence, including mutations that reduce efficacy or lead escape mutants. Surveillance should include both sequencing data an evaluation prophylactic antibodies, using clinical isolates. IMPORTANCE cause infections young worldwide. Recent progress has led ways prevent serious RSV-associated disease. virus’s protein key focus vaccine development because helps enter host cells well conserved across different However, small differences could affect behaves vitro . In study, we, therefore, samples under two who presented (12 8 RSV-B) testing, based their sequences. Phenotypic reference strains, such as stability at 4°C susceptibility neutralization, highlight viruses isolated recent samples. significant observed traits related protein, subgroups within, underlying molecular mechanisms remain unclear. Ongoing monitoring critical ensure current future vaccines effective.

Язык: Английский

---

Viral Infections in Elderly Individuals: A Comprehensive Overview of SARS-CoV-2 and Influenza Susceptibility, Pathogenesis, and Clinical Treatment Strategies

Vaccines, Год журнала: 2025, Номер 13(4), С. 431 - 431

Опубликована: Апрель 21, 2025

As age increases, the immune function of elderly individuals gradually decreases, increasing their susceptibility to infectious diseases. Therefore, further research on common viral infections in population, especially severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza viruses, is crucial for scientific progress. This review delves into genetic structure, infection mechanisms, impact coinfections with these two viruses provides a detailed analysis reasons increased dual infections. We evaluated clinical manifestations following coinfections, including complications respiratory, gastrointestinal, nervous, cardiovascular systems. Ultimately, we have summarized current strategies prevention, diagnosis, treatment SARS-CoV-2 older adults. Through studies, aim reduce risk provide basis age-related diseases, thereby improving health status.

Язык: Английский

---

Pharmacological mechanisms of probenecid for SARS-CoV-2 and RSV co-infection: findings of system pharmacology, molecular docking, molecular dynamics simulation, and structure–activity relationship

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Апрель 30, 2025

Background The clinical consequences of the co-infection with novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and syncytial virus (RSV) are not optimistic. Nevertheless, there is currently no approved therapeutic regimen specifically targeting SARS-CoV-2/RSV co-infection, existing monotherapies showing limited efficacy. According to recent studies, probenecid has both anti-SARS-CoV-2 anti-RSV effects. Therefore, as one probable molecular candidate for SARS-CoV-2 RSV, was researched in this exploration. Methods Using systems pharmacology bioinformatics, we characterized targets associated treatment focusing on their biological functions, mechanisms binding activities. To further validate these findings, conducted docking, MD simulations, electrostatic potential mapping, SAR analysis explore interactions between identified core targets. Results We 141 that overlapped probenecid, used shared construct a protein-protein interaction (PPI) network. Subsequently, obtained top 16 hub namely, AKT1, ALB, EGFR, CASP3, CTNNB1, SRC, HSP90AA1, so on. enrichment analysis, might affect inflammation, immunity, oxidative stress, defenses; Toll-like receptor, TNF, IL-17, NOD-like cytokine-cytokine among others. Additionally, based docking effectively bound related co-infection. Meanwhile, according dynamics (MD) simulations structure-activity relationship (SAR) speculated SRC HSP90AA1 more likely be target proteins than other proteins. Conclusion Our findings from bioinformatics indicate immune inflammatory responses play pivotal role effects probenecid. Infectious disease-related pathways also contribute significantly its effectiveness treating Further validation through analysis. These analyses suggest This study provides valuable preliminary insights into It establishes strong foundation future research strategy

Язык: Английский

---

Mitigating co-circulation of seasonal influenza and COVID-19 pandemic in the presence of vaccination: A mathematical modeling approach

Frontiers in Public Health, Год журнала: 2023, Номер 10

Опубликована: Янв. 4, 2023

The co-circulation of two respiratory infections with similar symptoms in a population can significantly overburden healthcare system by slowing the testing and treatment. persistent emergence contagious variants SARS-CoV-2, along imperfect vaccines their waning protections, have increased likelihood new COVID-19 outbreaks taking place during typical flu season. Here, we developed mathematical model for dynamics influenza, under different scenarios influenza vaccine coverage, booster coverage efficacy, capacity. We investigated required minimal optimal (third) fourth doses, conjunction vaccine, to avoid coincidence infection peaks both diseases single show that delay brought on high number cases impacts transmission. earlier peak season greater flu-like symptoms, risk transmission, which slows down testing, resulting complete isolation patients who not been isolated before clinical presentation continuing normal daily activities. Furthermore, our simulations stress importance uptake preventing infection, severe illness, hospitalization at individual level disease outbreak control putting strain already weak overwhelmed systems. As such, ensuring reduce burden these is paramount. showed keeping about 35% increasing or dose only reduces but also its time. If 55%, unexpectedly, it increases size slightly, while as well delays diseases. Mask-wearing coupled moderate increase may mitigate prevent an outbreak.

Язык: Английский

---

PHDtools: A platform for pathogen detection and multi-dimensional genetic signatures decoding to realize pathogen genomics data analyses online

Gene, Год журнала: 2024, Номер 909, С. 148306 - 148306

Опубликована: Фев. 24, 2024

Язык: Английский

---

Burden of Influenza and Respiratory Syncytial Viruses in Suspected COVID-19 Patients: A Cross-Sectional and Meta-Analysis Study

Viruses, Год журнала: 2023, Номер 15(3), С. 665 - 665

Опубликована: Март 1, 2023

Non-SARS-CoV-2 respiratory viral infections, such as influenza virus (FluV) and human syncytial (RSV), have contributed considerably to the burden of infectious diseases in non-COVID-19 era. While rates co-infection SARS-CoV-2-positive group (SCPG) patients been determined, other viruses SARS-CoV-2-negative (SCNG) remains unclear. Here, we conducted a cross-sectional study (São José do Rio Preto county, Brazil), collected our data using meta-analysis evaluate pooled prevalence FluV RSV among SCNG patients. Out 901 suspected COVID-19, molecular results showed positivity was 2% (15/733) 0.27% (2/733), respectively. Co-infection with SARS-CoV-2 FluV, or RSV, identified 1.7% (3/168). Following meta-analysis, 28 studies were selected (n = 114,318 COVID-19 patients), 4% (95% CI: 3–6) for 1–3) observed. Interestingly, four times higher (OR 4, 95% 3.6–5.4, p < 0.01) than SCPG. Similarly, significantly associated 2.9, 2–4, 0.01). For subgroup analysis, cold-like symptoms, including fever, cough, sore throat, headache, myalgia, diarrhea, nausea/vomiting, positively (p 0.05) In conclusion, these show that SCPG during early phase pandemic.

Язык: Английский

---

Characteristics of the pulmonary microbiota in patients with mild and severe pulmonary infection

Frontiers in Cellular and Infection Microbiology, Год журнала: 2023, Номер 13

Опубликована: Окт. 12, 2023

Background Lung infection is a global health problem associated with high morbidity and mortality increasing rates of hospitalization. The correlation between pulmonary microecology severity remains unclear. Therefore, the purpose this study was to investigate differences in lung potential biomarkers patients mild severe infection. Method Patients or suspected were divided into group (140 cases) (80 according pneomonia index (PSI) scores. Here, we used metagenomic next-generation sequencing (mNGS) detect DNA mainly from bronchoalveolar lavage fluid (BALF) collected analyze changes microbiome different disease severity. Result We mNGS microecological composition results alpha diversity beta analysis showed that microbial groups similar on whole. dominant bacteria Acinetobacter , Bacillus Mycobacterium Staphylococcus Prevotella among others. Linear discriminant effect size (LEfSe) there significant virus patients, especially Simplexvirus Cytomegalovirus, which prominent group. random forest model screened 14 kinds infection-related pathogens including Corynebacterium Streptococcus Klebsiella . In addition, it found Rothia negatively correlated Enterococcus through co-occurrence network, while no Conclusion describe A increase viral replication group, as well difference interactions infections, particularly association common pathogenic This suggests both pathogen co-viral may influence course disease. Of course, more research needed further explore specific mechanisms by

Язык: Английский

---

Epidemiological and Genetic Characteristics of Respiratory Viral Coinfections with Different Variants of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)

Viruses, Год журнала: 2024, Номер 16(6), С. 958 - 958

Опубликована: Июнь 13, 2024

This study aimed to determine the incidence and etiological, seasonal, genetic characteristics of respiratory viral coinfections involving severe acute syndrome coronavirus 2 (SARS-CoV-2). Between October 2020 January 2024, nasopharyngeal samples were collected from 2277 SARS-CoV-2-positive patients. Two multiplex approaches used detect sequence SARS-CoV-2, influenza A/B viruses, other seasonal viruses: real-time polymerase chain reaction (PCR) next-generation sequencing. Coinfections SARS-CoV-2 with viruses detected in 164 (7.2%) The most common co-infecting virus was syncytial (RSV) (38 cases, 1.7%), followed by bocavirus (BoV) (1.2%) rhinovirus (RV) (1.1%). Patients ≤ 16 years age had highest rate (15%) mixed infections. Whole-genome sequencing produced 19 complete genomes co-pathogens, which subjected phylogenetic amino acid analyses. classified into groups 6B.1A.5a.2a 6B.1A.5a.2a.1 for A(H1N1)pdm09, 3C.2a1b.2a.2a.1 3C.2a.2b A(H3N2), V1A.3a.2 B/Victoria lineage. RSV-B sequences belonged group GB5.0.5a, HAdV-C belonging type 1, BoV genotype VP1, PIV3 lineage 1a(i). Multiple substitutions identified, including at antibody-binding sites. provides insights reinforces importance characterization co-pathogens development therapeutic preventive strategies.

Язык: Английский

---

Detection of Torquetenovirus and Redondovirus DNA in Saliva Samples from SARS-CoV-2-Positive and -Negative Subjects

Viruses, Год журнала: 2022, Номер 14(11), С. 2482 - 2482

Опубликована: Ноя. 9, 2022

Objectives: Torquetenovirus (TTV) and Redondovirus (ReDoV) are the most prevalent viruses found in human respiratory virome viral metagenomics studies. A large-scale epidemiological study was performed to investigate their prevalence loads saliva samples according SARS-CoV-2 status. Methods: Saliva from 448 individuals (73% negative 27% positive) aged 23–88 years were tested. TTV determined by specific qualitative quantitative real-time PCRs, respectively. sub-cohort of 377 subjects additionally tested for presence load ReDoV saliva, a different 120 which paired plasma available viremia at same timepoints as saliva. Results: 72% entire cohort, mean DNA 4.6 log copies/mL, with no difference regardless 61% cohort more SARS-CoV-2-negative subgroup (65% vs. 52%, respectively). In total very similar one TTV, value 4.4 copies/mL. The infected single virus, namely, those or alone, lower than dually samples, Tukey’s multiple-comparison test showed that single-infected resulted only true outlier (p = 0.004). Differently not detected any blood samples. Conclusions: This establishes demonstrates existence differences between these two components virome.

Язык: Английский

---