Nature Communications,
Год журнала:
2017,
Номер
8(1)
Опубликована: Март 9, 2017
Many
biological
and
behavioural
processes
of
animals
are
governed
by
an
endogenous
circadian
clock,
which
is
dependent
on
transcriptional
regulation.
Here
we
address
post-transcriptional
regulation
the
role
miRNAs
in
Drosophila
rhythms.
At
least
six
show
cycling
expression
levels
within
pigment
dispersing
factor
(PDF)
cell-pacemaker
neurons;
only
mir-92a
peaks
during
night.
In
vivo
calcium
monitoring,
dynamics
PDF
projections,
ArcLight,
GCaMP6
imaging
sleep
assays
indicate
that
suppresses
neuronal
excitability.
addition,
cells
respond
to
light
pulses
also
affect
phase
shift
response.
Translating
ribosome
affinity
purification
(TRAP)
vitro
luciferase
reporter
assay
sirt2,
homologous
human
sir2
sirt3.
sirt2
RNAi
phenocopies
overexpression.
These
experiments
a
functional
target
modulates
excitability
via
suppressing
SIRT2
rhythmic
manner.
Genetics,
Год журнала:
2017,
Номер
205(4), С. 1373 - 1397
Опубликована: Март 30, 2017
The
advantages
of
the
model
organism
Drosophila
melanogaster,
including
low
genetic
redundancy,
functional
simplicity,
and
ability
to
conduct
large-scale
screens,
have
been
essential
for
understanding
molecular
nature
circadian
(∼24
hr)
rhythms,
continue
be
valuable
in
discovering
novel
regulators
rhythms
sleep.
In
this
review,
we
discuss
current
these
interrelated
biological
processes
wider
implications
research.
Clock
genes
period
timeless
were
first
discovered
screens
developed
1970s.
Feedback
on
their
own
transcription
forms
core
clock,
accurately
timed
expression,
localization,
post-transcriptional
modification,
function
is
thought
critical
maintaining
cycle.
Regulators,
several
phosphatases
kinases,
act
different
steps
feedback
loop
ensure
strong
rhythms.
Approximately
150
neurons
fly
brain
that
contain
components
clock
together
translate
intracellular
cycling
into
rhythmic
behavior.
We
how
groups
serve
functions
allowing
clocks
entrain
environmental
cues,
driving
behavioral
outputs
at
times
day,
flexible
responses
conditions.
neuropeptide
PDF
provides
an
important
signal
synchronize
neurons,
although
details
accomplishes
are
still
being
explored.
Secreted
signals
from
also
influence
other
tissues.
SLEEP
is,
part,
regulated
by
which
ensures
appropriate
timing
sleep,
but
amount
quality
sleep
determined
mechanisms
a
homeostatic
balance
between
wake.
Flies
useful
identifying
large
set
genes,
molecules,
neuroanatomic
loci
regulating
amount.
Conserved
aspects
regulation
flies
mammals
include
wake-promoting
roles
catecholamine
neurotransmitters
involvement
hypothalamus-like
regions,
regions
implicated
less
clear
parallels.
Sleep
subject
factors
such
as
food
availability,
stress,
social
environment.
beginning
understand
identified
molecules
interact
with
each
other,
environment,
regulate
researchers
can
take
advantage
increasing
mechanistic
behaviors,
learning
memory,
courtship,
aggression,
loss
impacts
behaviors.
thus
remain
tool
both
discovery
deep
PLoS Genetics,
Год журнала:
2017,
Номер
13(2), С. e1006613 - e1006613
Опубликована: Фев. 9, 2017
Locomotor
activity
rhythms
are
controlled
by
a
network
of
~150
circadian
neurons
within
the
adult
Drosophila
brain.
They
subdivided
based
on
their
anatomical
locations
and
properties.
We
profiled
transcripts
"around
clock"
from
three
key
groups
with
different
functions.
also
non-circadian
outgroup,
dopaminergic
(TH)
neurons.
have
cycling
but
fewer
than
clock
as
well
low
expression
poor
gene
transcripts.
This
suggests
that
TH
do
not
canonical
is
driven
brain
systemic
cues.
The
surprisingly
diverse
in
overall
patterns,
which
include
known
putative
novel
neuropeptides.
Even
phase
distributions
distinct,
indicating
regulatory
principles
govern
transcript
oscillations.
surprising
cell-type
diversity
parallels
functional
heterogeneity
Genes & Development,
Год журнала:
2016,
Номер
30(23), С. 2596 - 2606
Опубликована: Дек. 1, 2016
Circadian
clocks
regulate
much
of
behavior
and
physiology,
but
the
mechanisms
by
which
they
do
so
remain
poorly
understood.
While
cyclic
gene
expression
is
thought
to
underlie
metabolic
rhythms,
little
known
about
cycles
in
cellular
physiology.
We
found
that
Drosophila
insulin-producing
cells
(IPCs),
are
located
pars
intercerebralis
lack
an
autonomous
circadian
clock,
functionally
connected
central
clock
circuit
via
DN1
neurons.
Insulin
mediates
output
regulating
rhythmic
a
(sxe2)
fat
body.
Patch
clamp
electrophysiology
reveals
IPCs
display
clock-regulated
daily
rhythms
firing
event
frequency
bursting
proportion
under
light:dark
conditions.
The
activity
sxe2
additionally
regulated
feeding,
as
demonstrated
night
feeding-induced
changes
IPC
characteristics
levels
These
findings
indicate
circuit-level
regulation
metabolism
support
role
for
integrating
control
